Genetic diversity, virulotyping and antimicrobial resistance susceptibility of<i>Yersinia enterocolitica</i>isolated from pigs and porcine products in Malaysia

Thong, Kwai Lin; Tan, Lai Kuan; Ooi, Peck Toung

doi:10.1002/jsfa.8442

Cited by 16 publications

(20 citation statements)

References 39 publications

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“…Of note, the occurrence of an ampicillin resistance-encoding gene was reported, according to the literature, in isolates of non-clinical origin [43], a feature that was supported by the findings reported herein. Y.…”

Section: Resultssupporting

confidence: 88%

Comparative genomic insights into Yersinia hibernica – a commonly misidentified Yersinia enterocolitica-like organism

et al. 2020

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Food-associated outbreaks linked to enteropathogenic Yersinia enterocolitica are of concern to public health. Pigs and their meat are recognized risk factors for transmission of Y. enterocolitica . This study aimed to describe the comparative genomics of Y. enterocolitica along with a number of misclassified Yersinia isolates, now constituting the recently described Yersinia hibernica . The latter was originally cultured from an environmental sample taken at a pig slaughterhouse. Unique features were identified in the genome of Y. hibernica, including a novel integrative conjugative element (ICE), denoted as ICE Yh-1 contained within a 255 kbp region of plasticity. In addition, a zebrafish embryo infection model was adapted and applied to assess the virulence potential among Yersinia isolates including Y. hibernica .

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Section: Resultssupporting

confidence: 88%

Comparative genomic insights into Yersinia hibernica – a commonly misidentified Yersinia enterocolitica-like organism

et al. 2020

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“…Yersinia enterocolitica is a widely distributed enteropathogen, with pigs as major reservoirs (Thong, Tan, & Ooi, ). This microorganism is associated with intestinal, extraintestinal and immunological manifestations (Kato, Sasaki, & Sekiya, ; Sabina, Rahman, Ray, & Montet, ; Thwaites & Woods, ).…”

Section: Introductionmentioning

confidence: 99%

Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile

Mastrodonato

Favier

Estrada

et al. 2018

Journal of Food Safety

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Yersinia enterocolitica is associated with human clinical manifestations. In this study, the bioserotype distribution, virulence potential, the antimicrobial susceptibility, and the genomic diversity of Y. enterocolitica isolates recovered from Argentina and Chile were assessed. Eight Chilean and 22 Argentine isolates recovered from human (7) and animal feces (3), foods (18) and wastewater (2) were analyzed. They belonged to the bioserotypes B2 O:9 (9), B1A O:5 (8), B1A O:41,42‐41,43 (8), B4 O:3 (3) and B1A O:7,8‐8‐8,19 (2). Autoagglutination (AA), Ca2+‐dependent growth at 37°C and yadA, inv and yst genes were observed in B2 O:9 and B4 O:3 strains. B1A strains exhibited the genotype yadA‐ inv‐ yst+. All strains were resistant to ampicillin, rifampicin and erythromycin. By XbaI‐PFGE, 11 genomic types (GTs) were demonstrated (D.I. 0.90). The characteristics of these Y. enterocolitica isolates highlight the importance to maintain active surveillance of this enteropathogen in both countries. Practical applications The trade exchange among Chile and Argentina includes 12–19% of agricultural goods and foodstuffs with fresh and processed vegetables, seafoods and meat products as the major commodities. Additionally, travel and tourism have increased in recent years with more than 2.5 million passengers moving from Argentina to Chile in 2017. In this context, the control of foodborne pathogens and diseases represents a challenge for both countries. The analysis of bioserotypes, virulence potential, antimicrobial susceptibility, and clonal relatedness of Argentine and Chilean Y. enterocolitica isolates encourages further studies to contribute to the knowledge of the epidemiology of this enteropathogen and the implementation of adequate sanitary measures to prevent Y. enterocolitica illness in both countries.

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“…1,9 Regarding aminoglycosides, gentamicin, and streptomycin, low levels of resistance have been reported among Y. enterocolitica strains isolated in Iran, which is in accordance with reports from Italy (0%), China (4.3% to gentamicin and 8.6% to streptomycin), Austria (0%), Germany (0%), Turkey (0% to gentamicin and 4.8% to streptomycin), and Korea (0%). [25][26][27][28][29][30] The prevalence of resistance to other antibiotics used for the treatment of human yersiniosis (i.e., trimethoprim/sulfamethoxazole) was higher (17.1%) than those reported from Italy (0%) 25 and Malaysia, 31 but lower than that of China (74.3%). 26…”

Section: Discussionmentioning

confidence: 92%

“…Reported resistance prevalence of Y. enterocolitica strains isolated from food products against quinolones, ciprofloxacin and nalidixic acid, and tetracycline in different countries is as follows: Italy (0%), China (1.4% to ciprofloxacin, 18.6% to nalidixic acid, and 8.6% to tetracycline), Austria (0%), Germany (0%), Turkey (0% to ciprofloxacin, 4.8% to nalidixic acid, and 4.8% to tetracycline), Korea (0%), and Malaysia (62.5% to nalidixic acid and tetracycline). [25][26][27][28][29][30][31] Reports have proven the resistance of Y. enterocolitica to first-generation cephalosporins and ampicillin. 1 Similarly, the resistance of Y. enterocolitica to β-lactams including amoxicillin (45.9%), ampicillin (50.3%), and cephalothin (83.3%) was high in Iran.…”

Section: Discussionmentioning

confidence: 99%

“…25 Similar results in accordance with our meta-analysis were observed for Y. enterocolitica strains isolated from food products in Italy (100% to ampicillin and cephalothin), China (98.6% to ampicillin and 95.7% to cephalothin), Germany (83.3% to amoxicillin, 50% to ampicillin, and 83.3% to cephalothin), Turkey (57.1% to amoxicillin, 64.3% to ampicillin, and 86.9% to cephalothin), Korea (94% to ampicillin and 100% to cephalothin) and Malaysia (92.6% to amoxicillin and 96.3% to ampicillin). 25,26,[28][29][30][31] Gentamicin in combination with either fluoroquinolones or thirdgeneration cephalosporins was recommended in lifethreatening Y. enterocolitica infections, such as sepsis and meningitis. 1,9 Regarding aminoglycosides, gentamicin, and streptomycin, low levels of resistance have been reported among Y. enterocolitica strains isolated in Iran, which is in accordance with reports from Italy (0%), China (4.3% to gentamicin and 8.6% to streptomycin), Austria (0%), Germany (0%), Turkey (0% to gentamicin and 4.8% to streptomycin), and Korea (0%).…”

Section: Discussionmentioning

confidence: 99%

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A Systematic Review and Meta-analysis on the Epidemiology of Antibiotic-resistant Yersinia Species in Food and Clinical Specimens in Iran

Khademi

Sahebkar

2019

Int J Enteric Pathog

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Objective: The aim of the present study was to investigate the antimicrobial susceptibility profiles of Yersinia species, especially Y. enterocolitica from non-clinical and clinical isolates in Iran. Materials and Methods: We systematically searched PubMed, Scopus, Google Scholar, and the Scientific Information Database (SID) using "antibiotic resistance", "Yersinia", and "Iran" as major keywords until June 10, 2019. According to the predefined article selection criteria, published studies addressing the epidemiology of antibiotic-resistant Yersinia species in Iran were included in the meta-analysis. Data were extracted and exported to the Comprehensive Meta-Analysis Software to evaluate antibiotic resistance rates, heterogeneity of studies and publication bias. Results: Twelve studies reported antimicrobial susceptibility testing using disk diffusion method. The pooled prevalence of antibiotic-resistant Yersinia species in food and clinical specimens in Iran was as follows: 22.4% to amoxicillin, 41.9% to ampicillin, 6% to gentamicin, 17% to trimethoprim/ sulfamethoxazole, 19% to tetracycline, 10.3% to ciprofloxacin, 10.5% to streptomycin, 3.8% to chloramphenicol, 79.3% to cephalothin, 18.4% to nalidixic acid, 6.6% to cefotaxime, and 12.2% to trimethoprim. Conclusion: This study revealed a high prevalence of resistant Y. enterocolitica strains isolated from food and clinical specimens in Iran to β-lactams, while the resistance rates to aminoglycosides, fluoroquinolone and chloramphenicol were low. Our findings recommended the necessity of a continuous surveillance of the resistance patterns and prudent use of trimethoprim/ sulfamethoxazole, tetracycline, and nalidixic acid to prevent the development of antibioticresistant Y. enterocolitica strains in Iran.

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Genetic diversity, virulotyping and antimicrobial resistance susceptibility ofYersinia enterocoliticaisolated from pigs and porcine products in Malaysia

Abstract: BACKGROUND: The objectives of the present study were to determine the antimicrobial resistance, virulotypes and genetic diversity of Yersinia enterocolitica isolated from uncooked porcine food and live pigs in Malaysia.

Cited by 16 publications

References 39 publications

Comparative genomic insights into Yersinia hibernica – a commonly misidentified Yersinia enterocolitica-like organism

Comparative genomic insights into Yersinia hibernica – a commonly misidentified Yersinia enterocolitica-like organism

Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile

A Systematic Review and Meta-analysis on the Epidemiology of Antibiotic-resistant Yersinia Species in Food and Clinical Specimens in Iran

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