Genetic Engineering and Tissue Culture of Roses

Debener, Thomas; Oyant, Laurence Hibrand‐Saint

doi:10.1007/978-0-387-77491-6_19

Cited by 4 publications

(3 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ‘RI’ genotype was transformed with 35S::RoKSN proID::GFP, 35S::RoKSN::GFP , or with the empty vector ( 35S::GFP ). Rose transformation and regeneration are difficult, long and inefficient (Debener & Hibrand‐Saint Oyant, ). We succeeded in regenerating and acclimating two plants transformed with 35S::RoKSN proID :: GFP (D8.1 and D8.2 plants), five plants transformed with 35S::RoKSN::GFP (D6.1 to D6.5 plants) and six plants transformed with the empty vector (D7.1, D7.2, D7.3, D7.4, D7.5 and D5.1 plants).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

RoKSN, a floral repressor, forms protein complexes with RoFD and RoFT to regulate vegetative and reproductive development in rose

et al. 2013

View full text Add to dashboard Cite

SummaryFT/TFL1 family members have been known to be involved in the development and flowering in plants. In rose, RoKSN, a TFL1 homologue, is a key regulator of flowering, whose absence causes continuous flowering. Our objectives are to functionally validate RoKSN and to explore its mode of action in rose.We complemented Arabidopsis tfl1 mutants and ectopically expressed RoKSN in a continuous-flowering (CF) rose. Using different protein interaction techniques, we studied RoKSN interactions with RoFD and RoFT and possible competition.In Arabidopsis, RoKSN complemented the tfl1 mutant by rescuing late flowering and indeterminate growth. In CF roses, the ectopic expression of RoKSN led to the absence of flowering. Different branching patterns were observed and some transgenic plants had an increased number of leaflets per leaf. In these transgenic roses, floral activator transcripts decreased. Furthermore, RoKSN was able to interact both with RoFD and the floral activator, RoFT. Protein interaction experiments revealed that RoKSN and RoFT could compete with RoFD for repression and activation of blooming, respectively.We conclude that RoKSN is a floral repressor and is also involved in the vegetative development of rose. RoKSN forms a complex with RoFD and could compete with RoFT for repression of flowering.

show abstract

Section: Resultsmentioning

confidence: 99%

“…In rose, the complete functional validation of RoKSN can be obtained by downregulation of RoKSN in once-flowering roses by RNAi. However, the genetic transformation of OF roses is not possible at this time and protocols have only been developed on CF roses (Debener & Hibrand-Saint Oyant, 2009).…”

Section: Roksn Is a Floral Repressormentioning

confidence: 99%

RoKSN, a floral repressor, forms protein complexes with RoFD and RoFT to regulate vegetative and reproductive development in rose

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Functional validation still needs to be performed in rose by knocking out the gene in OF roses. Presently, no protocols for the genetic transformation of OF roses are available (Debener and Hibrand‐Saint Oyant, 2009). However, the conclusion drawn in rose was reinforced by the results obtained in woodland strawberry.…”

Section: Discussionmentioning

confidence: 99%

The TFL1 homologue KSN is a regulator of continuous flowering in rose and strawberry

et al. 2011

Self Cite

View full text Add to dashboard Cite

SUMMARYFlowering is a key event in plant life, and is finely tuned by environmental and endogenous signals to adapt to different environments. In horticulture, continuous flowering (CF) is a popular trait introduced in a wide range of cultivated varieties. It played an essential role in the tremendous success of modern roses and woodland strawberries in gardens. CF genotypes flower during all favourable seasons, whereas once-flowering (OF) genotypes only flower in spring. Here we show that in rose and strawberry continuous flowering is controlled by orthologous genes of the TERMINAL FLOWER 1 (TFL1) family. In rose, six independent pairs of CF/OF mutants differ in the presence of a retrotransposon in the second intron of the TFL1 homologue. Because of an insertion of the retrotransposon, transcription of the gene is blocked in CF roses and the absence of the floral repressor provokes continuous blooming. In OF-climbing mutants, the retrotransposon has recombined to give an allele bearing only the long terminal repeat element, thus restoring a functional allele. In OF roses, seasonal regulation of the TFL1 homologue may explain the seasonal flowering, with low expression in spring to allow the first bloom. In woodland strawberry, Fragaria vesca, a 2-bp deletion in the coding region of the TFL1 homologue introduces a frame shift and is responsible for CF behaviour. A diversity analysis has revealed that this deletion is always associated with the CF phenotype. Our results demonstrate a new role of TFL1 in perennial plants in maintaining vegetative growth and modifying flowering seasonality.

show abstract

Genetic dissection of adventitious shoot regeneration in roses by employing genome-wide association studies

et al. 2017

View full text Add to dashboard Cite

We analysed the capacity to regenerate adventitious shoots in 96 rose genotypes and found 88 SNP markers associated with QTLs, some of which are derived from candidate genes for shoot regeneration. In an association panel of 96 rose genotypes previously analysed for petal colour, we conducted a genome-wide association study on the capacity of leaf petioles for direct shoot regeneration. Shoot regeneration rate and shoot ratio (number of shoots/total number of explants) were used as phenotypic descriptors for regeneration capacity. Two independent experiments were carried out with six replicates of ten explants each. We found significant variation between the genotypes ranging from 0.88 to 88.33% for the regeneration rate and from 0.008 to 1.2 for the shoot ratio, which exceeded the rates reported so far. Furthermore, we found 88 SNP markers associated with either the shoot regeneration rate or the shoot ratio. In this association analysis, we found 12 SNP markers from ESTs (expressed sequence tags) matching known candidate genes that are involved in shoot morphogenesis. The best markers explained more than 51% of the variance in the shoot regeneration rate and more than 0.65 of the variance in the shoot regeneration ratio between the homozygote marker classes. The genes underlying some of the best markers such as a GT-transcription factor or an LRR receptor-like protein kinase are novel candidate genes putatively involved in the observed phenotypic differences. The associated markers were mapped to the closely related genome of Fragaria vesca and revealed many distinct clusters, which also comprised the known candidate genes that functioned in the organogenesis of plant shoots. However, the validation of candidate genes and their functional relationship to shoot regeneration require further analysis in independent rose populations and functional analyses.

show abstract

Genetic Engineering and Tissue Culture of Roses

Cited by 4 publications

References 67 publications

RoKSN, a floral repressor, forms protein complexes with RoFD and RoFT to regulate vegetative and reproductive development in rose

RoKSN, a floral repressor, forms protein complexes with RoFD and RoFT to regulate vegetative and reproductive development in rose

The TFL1 homologue KSN is a regulator of continuous flowering in rose and strawberry

Genetic dissection of adventitious shoot regeneration in roses by employing genome-wide association studies

Contact Info

Product

Resources

About