Genetic Engineering of a Zeaxanthin-rich Potato by Antisense Inactivation and Co-suppression of Carotenoid Epoxidation

Römer, Susanne; Lübeck, Jens; Kauder, Friedrich; Steiger, Sabine; Adomat, Christel; Sandmann, Gerhard

doi:10.1006/mben.2002.0234

Cited by 229 publications

(122 citation statements)

References 46 publications

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“…GM lines SR47 ('Baltica' cosuppression) and SR48 ('Baltica' antisense) accumulate the carotenoid zeaxanthin in their tubers. The tubers of GM lines SR47 and SR48 (referred to by Römer et al [34] as SR47-18 and SR48-17, respectively) contained up to 40 g/g (dry weight [dw]) and 17 g/g (dw) of zeaxanthin, compared to 0.2 g/g (dw) for 'Baltica'.…”

Section: Methodsmentioning

confidence: 99%

“…Zeaxanthin is naturally produced in potato plants but is further modified to violaxanthin via the enzyme zeaxanthin epoxidase. Downregulation of the zeaxanthin epoxidase gene resulted in accumulation of zeaxanthin in tubers of GM potato plants (34). However, the possibility that additional plant metabolic processes, as well as root exudation patterns, are affected by the genetic modifications cannot be excluded.…”

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confidence: 99%

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Rhizosphere Communities of Genetically Modified Zeaxanthin-Accumulating Potato Plants and Their Parent Cultivar Differ Less than Those of Different Potato Cultivars

Weinert

Meincke

Gottwald

et al. 2009

Appl Environ Microbiol

126

111

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The effects of genetically modified (GM), zeaxanthin-accumulating potato plants on microbial communities in the rhizosphere were compared to the effects of different potato cultivars. Two GM lines and their parental cultivar, as well as four other potato cultivars, were grown in randomized field plots at two sites and in different years. Rhizosphere samples were taken at three developmental stages during plant growth and analyzed using denaturing gradient gel electrophoresis (DGGE) fingerprints of Bacteria, Actinobacteria, Alpha-and Betaproteobacteria, Bacillus, Streptomycetaceae, Pseudomonas, gacA, Fungi, and Ascomycetes. In the bacterial DGGE gels analyzed, significant differences between the parental cultivar and the two GM lines were detected mainly for Actinobacteria but also for Betaproteobacteria and Streptomycetaceae, yet these differences occurred only at one site and in one year. Significant differences occurred more frequently for Fungi, especially Ascomycetes, than for bacteria. When all seven plant genotypes were compared, DGGE analysis revealed that different cultivars had a greater effect on both bacterial and fungal communities than genetic modification. The effects of genetic modification were detected mostly at the senescence developmental stage of the plants. The site was the overriding factor affecting microbial community structure compared to the plant genotype. In general, the fingerprints of the two GM lines were more similar to that of the parental cultivar, and the differences observed did not exceed natural cultivar-dependent variability.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Rhizosphere Communities of Genetically Modified Zeaxanthin-Accumulating Potato Plants and Their Parent Cultivar Differ Less than Those of Different Potato Cultivars

Weinert

Meincke

Gottwald

et al. 2009

Appl Environ Microbiol

126

111

View full text Add to dashboard Cite

show abstract

“…Although the signals and metabolites involved in the feedback communication between the carotenoid pathway and the MEP pathway in Arabidopsis seedlings are still a matter of speculation (Laule et al, 2003;Guevara-Garcia et al, 2005;Rodriguez-Villalon et al, 2009a), it is likely that the upregulation of DXS both transcriptionally (in response to light signals and indirectly mediated by PIFs) and post-transcriptionally (in response to metabolic feedback triggered by PSY upregulation) ensures an appropriate supply of MEP-derived precursors for the biosynthesis of carotenoids during the critical stages of early seedling development in the light. Feedback regulation of carotenoid biosynthetic gene expression and enzyme activities by phytoene, lycopene, or/and other carotenoid metabolites has been proposed in many works (Misawa et al, 1994;Corona et al, 1996;Römer et al, 2000;Romer et al, 2002;Simkin et al, 2003;Diretto et al, 2006;Cuttriss et al, 2007;Qin et al, 2007;Harjes et al, 2008;Apel and Bock, 2009;Bai et al, 2009;Cazzonelli et al, 2009), but clear-cut evidence of the molecular mechanisms and metabolites involved is still missing.…”

Section: Modulation Of Enzyme Levels and Activitiesmentioning

confidence: 99%

Carotenoid Biosynthesis in Arabidopsis: A Colorful Pathway

Ruiz-Sola

Rodríguez‐Concepción

2012

The Arabidopsis Book

476

372

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Plant carotenoids are a family of pigments that participate in light harvesting and are essential for photoprotection against excess light. Furthermore, they act as precursors for the production of apocarotenoid hormones such as abscisic acid and strigolactones. In this review, we summarize the current knowledge on the genes and enzymes of the carotenoid biosynthetic pathway (which is now almost completely elucidated) and on the regulation of carotenoid biosynthesis at both transcriptional and post-transcriptional levels. We also discuss the relevance of Arabidopsis as a model system for the study of carotenogenesis and how metabolic engineering approaches in this plant have taught important lessons for carotenoid biotechnology.

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“…All other samples were separated on a Nucleosil C18 3 column with the same mobile phase at 25°C. In each case zeaxanthin and lutein were separated in parallel runs on a C18 Vydac 218TP54 column with methanol as the mobile phase (46). Samples were monitored with a Kontron DAD 440 photodiode array detector with on-line registration of the spectra.…”

Section: Dna Analysis Of Transgenic Plantsmentioning

confidence: 99%

Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize

Zhu

Naqvi

Breitenbach

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

332

263

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Combinatorial nuclear transformation is a novel method for the rapid production of multiplex-transgenic plants, which we have used to dissect and modify a complex metabolic pathway. To demonstrate the principle, we transferred 5 carotenogenic genes controlled by different endosperm-specific promoters into a white maize variety deficient for endosperm carotenoid synthesis. We recovered a diverse population of transgenic plants expressing different enzyme combinations and showing distinct metabolic phenotypes that allowed us to identify and complement rate-limiting steps in the pathway and to demonstrate competition between ␤-carotene hydroxylase and bacterial ␤-carotene ketolase for substrates in 4 sequential steps of the extended pathway. Importantly, this process allowed us to generate plants with extraordinary levels of ␤-carotene and other carotenoids, including complex mixtures of hydroxycarotenoids and ketocarotenoids. Combinatorial transformation is a versatile approach that could be used to modify any metabolic pathway and pathways controlling other biochemical, physiological, or developmental processes.induced mutation ͉ metabolic engineering ͉ transgenic plant ͉ provitamin A

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Genetic Engineering of a Zeaxanthin-rich Potato by Antisense Inactivation and Co-suppression of Carotenoid Epoxidation

Cited by 229 publications

References 46 publications

Rhizosphere Communities of Genetically Modified Zeaxanthin-Accumulating Potato Plants and Their Parent Cultivar Differ Less than Those of Different Potato Cultivars

Rhizosphere Communities of Genetically Modified Zeaxanthin-Accumulating Potato Plants and Their Parent Cultivar Differ Less than Those of Different Potato Cultivars

Carotenoid Biosynthesis in Arabidopsis: A Colorful Pathway

Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize

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