Genetic engineering of rice to resist rice tungro disease

Abstract

Rice tungro disease (RTD), caused by the coinfection of rice tungro bacilliform virus (RTBV) and rice tungro spherical virus, is one of the most important viral diseases of rice in South and Southeast Asia. The disease remains one of the major threats to sustainable rice production in many countries. The lack of resistance genes to RTBV-the causal agent of tungro disease-makes it even more difficult to manage RTD. In this review, we summarize previous and current research efforts to genetically engineer rice in order to increase the crop's resistance to tungro disease, including the use of pathogen-derived resistance and of host genes that confer RTD resistance and/ or that restrict feeding by the insect vector. The prospects of developing rice cultivars with durable resistance to RTD are also discussed.

Small RNA Based Genetic Engineering for Plant Viral Resistance: Application in Crop Protection

“…Since late 1980s in central and east Africa, cassava crops in almost 12 different countries were damaged due to cassava mosaic disease caused by cassava mosaic virus (CsMV) (Legg et al, 2011). In Southeast Asia, Rice tungro virus has been estimated to cause an annual loss of 5–10% of the rice yield (Dai and Beachy, 2009). Broad range of plants including tobacco, tomato, and peanuts has been infected by TSWV (Sherwood et al, 2003) and as a result, the annual economic losses due to this virus are projected to be one billion dollars worldwide (Roger, 2014).…”

Detection of probable marker-free transgene-positive rice plants resistant to rice tungro disease from backcross progenies of transgenic Pusa Basmati 1

“…RTD is reported to be responsible for 5-10% annual losses of rice yield in Asia (Dai and Beachy 2009) and about 2% in India (Muralidharan et al 2003). Due to the economic significance of RTD, incorporation of resistance has been an important objective in rice improvement programme in Asia.…”

“…Most attempts to develop RTD resistance through classical breeding have not been durable (Dahal et al 1990;Azzam and Chancellor 2002;Khush et al 2004). Biotechnological approaches have also been employed to develop transgenic plants for resistance against RTBV and RTSV (for review see Dai and Beachy 2009). The transgenic strategies for RTD resistance are promising, although pathogen derived resistance for RTD has reported as being only partially effective * For correspondence.…”

“…E-mail: sroypbr@gmail.com. (Dai and Beachy 2009). Recently, some work on the development of transgenic resistance to RTD have been done targeting RTBV, as it is the causative agent of tungro symptoms (Dai et al 2008;Tyagi et al 2008;Ganesan et al 2009).…”

Kendali Ketahanan Genetik Padi terhadap Penyakit Tungro

“…Kedua virus secara taksonomi berbeda dan dapat ditularkan oleh beberapa jenis wereng hijau (Nephotettix spp) secara semipersisten (Dai and Beachy 2009).…”