Genetic Engineering of Vegetable Crops

Sidhu, J. S.; Chellan, Sudarshan

doi:10.1002/9780470958346.ch4

Cited by 2 publications

(2 citation statements)

References 135 publications

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“…The use of genetic engineering to alter the traits of crop plants is well established and is of major biotechnological interest ( Moose and Mumm, 2008 ; Sidhu and Chellan, 2010 ). Following the commercial introduction of crop plants such as Roundup Ready soya in 1996 and corn in 1998, the so-called second generation of genetically engineered (GE) crops is primarily aimed at improving the nutrient status of crop plants for the consumer (reviewed by Mattoo et al ., 2010 ; McGloughlin, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Proteome changes in tomato lines transformed with phytoene synthase-1 in the sense and antisense orientations

Robertson

Koistinen

Gerrish³

et al. 2012

Journal of Experimental Botany

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The commercial cultivation of genetically engineered (GE) crops in Europe has met with considerable consumer resistance, which has led to vigorous safety assessments including the measurement of substantial equivalence between the GE and parent lines. This necessitates the identification and quantification of significant changes to the metabolome and proteome in the GE crop. In this study, the quantitative proteomic analysis of tomato fruit from lines that have been transformed with the carotenogenic gene phytoene synthase-1 (Psy-1), in the sense and antisense orientations, in comparison with a non-transformed, parental line is described. Multidimensional protein identification technology (MudPIT), with tandem mass spectrometry, has been used to identify proteins, while quantification has been carried out with isobaric tags for relative and absolute quantification (iTRAQ). Fruit from the GE plants showed significant alterations to their proteomes compared with the parental line, especially those from the Psy-1 sense transformants. These results demonstrate that MudPIT and iTRAQ are suitable techniques for the verification of substantial equivalence of the proteome in GE crops.

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Section: Introductionmentioning

confidence: 99%

Proteome changes in tomato lines transformed with phytoene synthase-1 in the sense and antisense orientations

Robertson

Koistinen

Gerrish³

et al. 2012

Journal of Experimental Botany

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“…These systems are characterized by the use of synthetic chemical inputs, mechanized management practices, and the dependence on an increasingly small number of hybrid varieties for its major crops (Mendum and Glenna, 2010). Furthermore, many genetically engineered (GE) vegetables are currently under various stages of trial and/or commercialization (Sidhu and Chellan, 2010). GE sweet corn, for example, has been grown commercially in the United States since 2012, and was developed to tolerate direct applications of glyphosate herbicide as well as to produce endogenous Cry insecticidal proteins (Seminis, 2013).…”

Section: Introductionmentioning

confidence: 99%

Rendimiento y calidad de fruta de cultivares de tomate de polinización abierta e híbridos cultivados bajo manejo orgánico en Puerto Rico.

Brady¹,

Brunner²,

Morales-Payán³

et al. 2013

JAUPR

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Variety trials were conducted in 2010 and 2011 to evaluate the yield and quality performance of 11 open pollinated and three hybrid tomato (Solarium lycopersicum L.) cultivars grown organically in Lajas, Puerto Rico. In both years, significant differences in marketable fruit yield were observed. Yields in 2010 ranged from 72.1 Mg/ha for 'Roma' to 8.6 Mg/ha for 'Marion'. 'Roma' and 'Neptune' (65.6 Mg/ha) were among the highest yielding cultivars. In 2011, significant yield differences were also observed. Marketable fruit yield ranged from 54.7 Mg/ha for 'Early Girl' to 23.5 Mg/ha for 'Super Sioux'. 'Early Girl', 'Flora Dade' (53.8 Mg/ha), 'BHN 444' (50.8 Mg/ha), 'Roma' (48.6 Mg/ha), 'Eva Purple Ball' (48.4 Mg/ha), 'Traveler 76' (46.4 Mg/ha) and 'Celebrity' (45.9 Mg/ha) were among the highest yielders. 'Early Girl' was consistently among the top cultivars in both seasons for taste, yield, and fruit appearance. Open pollinated cultivars performed with more variability between the two seasons than the hybrids. The open pollinated cultivars which ranked highest during the two-year study for taste, yield, and overall appearance were 'Roma', 'Homestead 24', 'Ozark Pink', and 'Traveler 76'. This study indicates that tomato can be grown successfully under an organic management system in Puerto Rico using either open pollinated cultivars or hybrids.

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Genetic Engineering of Vegetable Crops

Cited by 2 publications

References 135 publications

Proteome changes in tomato lines transformed with phytoene synthase-1 in the sense and antisense orientations

Proteome changes in tomato lines transformed with phytoene synthase-1 in the sense and antisense orientations

Rendimiento y calidad de fruta de cultivares de tomate de polinización abierta e híbridos cultivados bajo manejo orgánico en Puerto Rico.

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