Genetic Environment of blaTEM-1, blaCTX-M-15, blaCMY-42 and Characterization of Integrons of Escherichia coli Isolated From an Indian Urban Aquatic Environment

Singh, Nambram Somendro; Singhal, Neelja; Virdi, Jugsharan Singh

doi:10.3389/fmicb.2018.00382

Cited by 54 publications

(51 citation statements)

References 61 publications

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“…Although the isolates in CTX-M-1 group were not identified any IS due to the short length of contigs (Figure 8B), most isolates in other groups, such as CMY-2, CTX-M-27, CTX-M-32, CTX-M-15, and CTX-M-55, carried IS1380 family insertion sequences in common, and the IS1380 element was located upstream of CTX-M and CMY-2 genes (Figures 8A,C,D). In particular, the isolates encoding CMY-2 gene harbored IS in the upstream and sugE gene in the downstream as previously reported (Singh et al, 2018). Furthermore, the set of tra genes related to conjugal transfer system was co-identified in the isolates with CMY-2 ( Figure 8A).…”

Section: Characterization Of Ctx-m and Cmy-2 Genes On E Colisupporting

confidence: 76%

Prevalence and Molecular Characteristics of Extended-Spectrum and AmpC β-Lactamase Producing Escherichia coli in Grazing Beef Cattle

et al. 2020

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The emergence of extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase producing Escherichia coli represent a contemporary public health threat. ESBL and AmpC β-lactamase genes translocate between chromosomes and plasmids, facilitating rapid spread throughout the environment. In this study, ESBL/AmpC producing bacteria were isolated from beef cattle farms with seldom antibiotic use. Eleven farms out of 17 tested, had ESBL/AmpC producing E. coli in animals, soil, and forage samples. Fifty-nine CTX-M or CMY-2 positive E. coli isolates were further characterized with whole-genome sequencing. The isolates commonly carried CMY-2, TEM, or CTX-M genes, and over half encoded both CTX-M and TEM genes. Using comparative genomics, antimicrobial resistance genes from 12 classes of antimicrobial were identified and confirmed by antibiotic susceptibility test, revealing multidrug resistance against multiple classes of antibiotics. Virulence factors related to adherence, invasion, iron uptake, and bacterial secretion systems were shared by all isolates; some of which were identified as enteropathogenic E. coli. Phylogenetic analyses revealed a pattern of close genetic relatedness, suggesting that ESBL/AmpC producing E. coli were transmitted among farms as well as independent evolution within farms. Our results indicate that ESBL and AmpC β-lactamases prevail in food animal production system regardless antibiotic use and have the characteristics for zoonotic transmission.

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Section: Characterization Of Ctx-m and Cmy-2 Genes On E Colisupporting

confidence: 76%

Prevalence and Molecular Characteristics of Extended-Spectrum and AmpC β-Lactamase Producing Escherichia coli in Grazing Beef Cattle

et al. 2020

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“…In this study, 9 (52.9%) E. coli ST131 isolates transferred integron and resistance gene cassettes to E. coli J53 Az r by conjugation. Many previous studies found that most of the resistance determination and integron in E. coli were encoded in a transferable plasmid, which might be horizontally transferred to the isolates among E. coli ST131 or different bacteria species by conjugation, potentially resulting in multiple drug resistance of bacteria [36,37]. e result of PFGE demonstrated that integron-positive isolates were highly diverse in this study and there was no dominant clone ( Figure 1).…”

Section: Discussionmentioning

confidence: 54%

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

Huang

Lan

et al. 2020

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Background. Escherichia coli sequence type 131 (ST131) is an important multidrug-resistant extraintestinal pathogen, which can cause many kinds of infections. Integrons may play a crucial role in the dissemination of antibiotic resistance genes. e purpose of this study was to characterize the prevelance of integrons among E. coli ST131 strains in China. Methods. Eighty-three E. coli ST131 isolates were used in this study. e antibiotic susceptibility test was performed by the disk diffusion method. e presence and characterization of class 1, 2, and 3 integrons, as well as promotor of gene cassettes and other antimicrobial resistance genes, were detected by PCR and DNA sequencing. Transfer of integrons was carried out using a broth culture mating method. Clonal relatedness of E. coli ST131 isolates was analyzed by PFGE. Results. Overall, 26.5% (22/83) of the E. coli ST131 isolates carried class 1 integrons. Class 2 and 3 integrons were not found in this study. Two types of gene cassette arrays were demonstrated in this study and were as follows: dfrA17-aadA5 and aac(6′)-Ib-cr-cmlA5. Only one type of Pc promoter variant was detected among 22 integron-positive isolates (PcW). In vivo transfer of integron was successful for 9 of integron-positive E. coli ST131 isolates harboring resistance gene cassettes. Results of PFGE demonstrated that the integron-positive E. coli ST131 isolates were grouped into 12 different PFGE clusters. Conclusions. Our study showed a low prevalence of integrons was detected in E. coli ST131. Continued surveillance of this mobile genetic element should be performed to study the evolution of antibiotic resistance among E. coli ST131.

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“…coli ST5377, ST361 and ST672 collected in India between 2012 and 2013 [ 20 ]. Studying its genetic environment, we observed upstream of the bla CMY-42 the two genes previously reported by Singh et al (2018) [ 21 ]: blc and sugE encoding for an outer membrane lipoprotein and a drug efflux pump, respectively. We additionally detected an intact IS Ecp1 upstream of bla CMY-42 , which was not as previously shown by Hentschke et al (2011) [ 22 ] disrupted by IS 1 .…”

Section: Discussionmentioning

confidence: 78%

Molecular characterization of Carbapenem resistant Escherichia coli recovered from a tertiary hospital in Lebanon

et al. 2018

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The emergence of carbapenem resistant Escherichia coli represents a serious public health concern. This study investigated the resistome, virulence, plasmids content and clonality of 27 carbapenem resistant E. coli isolated from 27 hospitalized patients at the American University of Beirut Medical Center (AUBMC) in Lebanon between 2012 and 2016. Whole-genome sequencing (WGS) data were used to identify resistance determinants. Multilocus sequence typing (MLST), pulsed field gel electrophoresis (PFGE), phylogenetic grouping and PCR-based replicon typing (PBRT) were also performed. The 27 isolates were distributed into 15 STs, of which ST405 (14.8%; n = 4) was the most prevalent. All of the 27 isolates were carbapenem resistant and 20 (74%) were extended-spectrum β-lactamase (ESBL) gene carriers. The predominant detected carbapenemases were blaOXA-48 (48.1%; n = 13) and blaOXA-181 (7.4%; n = 2), for the ESBLs it was blaCTX-M-15 (55.6%; n = 15) and blaCTX-M-24 (18.5%; n = 5), and for the AmpC-type β–lactamases, blaCMY-42 (40.7%; n = 11) and blaCMY-2 (3.7%; n = 1). Thirteen replicons were identified among the 27 E. coli isolates including: IncL/M, IncFIA, IncFIB, IncFII, IncI1, and IncX3. PFGE revealed a high genetic diversity with the 27 isolates being grouped in 21 different pulsotypes. SNPs analysis and PFGE showed a possible clonal dissemination of ST405, ST1284, ST354 and ST410 and the dominance of certain STs, monitoring of which could help in elucidating routes of transmission. This study represents the first WGS-based in depth analysis of the resistomes and mobilomes of carbapenem resistant E. coli in Lebanon.

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Genetic Environment of blaTEM-1, blaCTX-M-15, blaCMY-42 and Characterization of Integrons of Escherichia coli Isolated From an Indian Urban Aquatic Environment

Cited by 54 publications

References 61 publications

Prevalence and Molecular Characteristics of Extended-Spectrum and AmpC β-Lactamase Producing Escherichia coli in Grazing Beef Cattle

Prevalence and Molecular Characteristics of Extended-Spectrum and AmpC β-Lactamase Producing Escherichia coli in Grazing Beef Cattle

Characterization of Integrons and Antimicrobial Resistance in Escherichia coli Sequence Type 131 Isolates

Molecular characterization of Carbapenem resistant Escherichia coli recovered from a tertiary hospital in Lebanon

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