Genetic evidence that the tryptophan 2-mono-oxygenase gene of Pseudomonas savastonoi is functionally equivalent to one of the T-DNA genes involved in plant tumour formation by Agrobacterium tumefaciens

Follin, A.; Inzé, Dirk; Budar, Françoise; Genetello, C.; Montagu, Marc Van; Schell, J.

doi:10.1007/bf00425657

Cited by 46 publications

(27 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The phenotype change in other plants with auxin transgenes have also been described. Tobacco transgenic plants with Agrobacterium tumefacience tms1 gene (homologous to aux1) produced phenotypically normal plants under the control of its own promoter [12], whereas the tms1 gene under the control of CaMV 19S promoter gave petunia and tobacco plants an extremely abnormal morphology [11]. In our present experiment, AUX genes consisted of aux1 and aux2 under the control of their own promoters gave Chinese cabbage plants either normal or extremely abnormal morphology.…”

Section: Discussionmentioning

confidence: 43%

Leafy head formation of the progenies of transgenic plants of Chinese cabbage with exogenous auxin genes

Xue

Sun

et al. 2000

Cell Res

View full text Add to dashboard Cite

The experiment was performed to evaluate the progenies of plant lines transgenic for auxin synthesis genes derived from Ri T-DNA. Four lines of the transgenic plants were self-crossed and the foreign auxin genes in plants of T5 generation were confirmed by Southern hybridization. Two lines, D1232 and D1653, showed earlier folding of expanding leaves than untransformed line and therefore had early initiation of leafy head. Leaf cuttings derived from plant of transgenic line D1653 produced more adventitious roots than the control whereas the cuttings from folding leaves had much more roots than rosette leaves at folding stage, and the cuttings from head leaves had more roots than rosette leaves at heading stage. It is demonstrated that early folding of transgenic leaf may be caused by the relatively higher concentration of auxin. These plant lines with auxin transgenes can be used for the study of hormonal regulation in differentiation and development of plant organs and for the breeding of new variety with rapid growth trait.

show abstract

Section: Discussionmentioning

confidence: 43%

Leafy head formation of the progenies of transgenic plants of Chinese cabbage with exogenous auxin genes

Xue

Sun

et al. 2000

Cell Res

View full text Add to dashboard Cite

show abstract

“…Plants expressing these IAA biosynthetic genes from promoters of different strength and tissue specificity could serve as a model system to manipulate endogenous IAA levels in vivo. Follin et al (4) showed that tobacco plants expressing either the iaaM or the iaaH gene from their natural promoters displayed a normal phenotype. However, by substituting the natural promoter with the much stronger CaMV 19S promoter, Klee et al (15) were able to show that transgenic petunia expressing a CaMV 19S-iaaM construct had a 10-fold IAA increase in leaf tissue.…”

mentioning

confidence: 99%

Transgenic Tobacco Plants Coexpressing the Agrobacterium tumefaciens iaaM and iaaH Genes Display Altered Growth and Indoleacetic Acid Metabolism

Sitbon

Hennion²,

Sundberg³

et al. 1992

Plant Physiol.

133

View full text Add to dashboard Cite

“…The genes for tryptophan 2-monooxygenase (iaaM) and indole-3-acetamide hydrolase (iaaH) are organized in an operon, and the only bacterium in which they function is P. savastanoi (4,5), whereas the comparable genes in A. tumefaciens, tms-i, and tms-2, are monocistronic and function only in plants (11,15,19,21,(28)(29)(30). Recently, significant homology between the gene iaaM and the gene tms-i and between the gene iaaH and the gene tms-2 was found in terms of the nucleotide sequences and deduced amino acid sequences (33).…”

Section: Discussionmentioning

confidence: 99%

“…Non-auxindependent growth of tumors is responsible for high concentrations of IAA produced by the IAM pathway located in the T-DNA (19). The T-DNA genes tms-i and tms-2 encode enzymes for the IAM pathway and are functional only in plants (11). Since the IAM pathway is not found in plants (21), the origin of this pathway is of great interest with respect to the evolutionary relationship between procaryotic and eucaryotic genes.…”

mentioning

confidence: 99%

Molecular cloning of a gene for indole-3-acetamide hydrolase from Bradyrhizobium japonicum

1989

View full text Add to dashboard Cite

A pLAFRl cosmid genomic library of wild-type Bradyrhizobiumrjaponicum J1063 was const e. A cosmid clone designated pJjJ4, containing a 26-kilobase (kb) DNA It has been shown that bacterial production of the phytohormone indoleacetic acid (IAA) is involved ih the virulence df several interactions between microorganisms and plants (19). Several pathways have been reported for the conversion of tryptophan to IAA in bacteria (27). The best-studied pathways involve conversion via indole-3-acetamide (IAM) (the IAM pathway) in Pseudomonas savastanoi and in Agrobacterium tumefaciens. In these pathways, tryptophan 2-monooxygenase converts tryptophan to IAM, and then indole-3-acetamide hydrolase catalyzes the conversion of IAM to IAA. P. savastanoi is a pathogen that infects olive and oleander plants, inducing tumorous outgrowths called olive (or oleander) knots. The formation of galls is dependent on the bacterial production of IAA, and the severity of the disease is correlated with the amounts of IAA secreted by bacteria through the IAM pathway (4, 5). The genes for the IAM pathway, termed the iaaM and iaaH genes, are organized in an operon which is located on a pIAA plasmid and are expressed only in bacteria (4, 5).A. tumefaciens induces tumors called crown gall on most dicotyledonous plants by integration of DNA into the plant genome and subsequent expression of a portion of the Ti

show abstract

Genetic evidence that the tryptophan 2-mono-oxygenase gene of Pseudomonas savastonoi is functionally equivalent to one of the T-DNA genes involved in plant tumour formation by Agrobacterium tumefaciens

Cited by 46 publications

References 42 publications

Leafy head formation of the progenies of transgenic plants of Chinese cabbage with exogenous auxin genes

Leafy head formation of the progenies of transgenic plants of Chinese cabbage with exogenous auxin genes

Transgenic Tobacco Plants Coexpressing the Agrobacterium tumefaciens iaaM and iaaH Genes Display Altered Growth and Indoleacetic Acid Metabolism

Molecular cloning of a gene for indole-3-acetamide hydrolase from Bradyrhizobium japonicum

Contact Info

Product

Resources

About