Leafy head formation of the progenies of transgenic plants of Chinese cabbage with exogenous auxin genes

He, Yu; Xue, Wan; Sun, Yu; Yu, Xu; Liu, Ping Lin

doi:10.1038/sj.cr.7290044

Cited by 55 publications

(54 citation statements)

References 9 publications

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“…High frequencies of resistant callus production and plantlet regeneration were obtained on G418-containig medium (Tab 2). This conclusion is in contrast to that of Raineri [16], Chan [17] and He [18]. In our study, the high efficiency of obtaining transgenic plants was obtained in immature embryos infected with EHA101/pExT 7 preinduced with AS followed by cocultivation in the presence of AS.…”

Section: Co-cultivation Selection and Plant Regenerationcontrasting

confidence: 52%

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

HUANG

Wei

et al. 2001

Cell Res

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Immature embryos of rice varieties Xiushui11 and Chunjiang 11 precultured for 4d were infected and transformed by Agrobacterium tumefaciens strain EHA101/pExT 7 (containing the spider insecticidal gene). The resistant calli were transferred onto the differentiation medium and plants were regenerated. The transformation frequency reached 56% ~ 72% measured as numbers of Geneticin (G418)-resistant calli produced and 36% ~ 60% measured as numbers of transgenic plants regenerated, respectively. PCR and Southern blot analysis of transgenic plants confirmed that the T-DNA had been integrated into the rice genome. Insect bioassays using T1 transgenic plants indicated that the mortality of the leaffolder (Cnaphalocrasis medinalis) after 7d of leaf feeding reached 38% ~ 61% and the corrected mortality of the striped stem borer (Chilo suppressalis) after 7d of leaf feeding reached 16% ~ 75%. The insect bioassay results demonstrated that the transgenic plants expressing the spider insecticidal protein conferred enhanced resistance to these pests.

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Section: Co-cultivation Selection and Plant Regenerationcontrasting

confidence: 52%

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

HUANG

Wei

et al. 2001

Cell Res

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“…In contrast, many Brassica crops such as Chinese cabbage and cabbage undergo vegetative growth in natural conditions for long period (>5 months). For example, the vegetative growth of Chinese cabbage is divided into four stages: seedling, rosette, folding, and heading (He et al , 2000), each of which is characterized by timing of abaxial trichomes, blade shape, and petiole length. A seedling stage is necessary for the formation of nutrient-rich heads.…”

Section: Discussionmentioning

confidence: 99%

HYL1 controls the miR156-mediated juvenile phase of vegetative growth

Li¹,

Yang²,

Wu³

et al. 2012

Journal of Experimental Botany

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HYL1 is an important regulator of microRNA (miRNA) biogenesis. A loss-of-function mutation of HYL1 causes the reduced accumulation of some miRNAs but fails to display the miRNA-deficient phenotypes of these miRNAs. In Arabidopsis, miR156 mediates phase transition through repression of SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) genes. However, it remains unknown whether, and if so how, HYL1 enables phase transition through miR156. This study showed that a loss-of-function mutation of the HYL1 gene caused defects in the timing of the juvenile phase. In the primary leaves of hyl1-2 mutants, abaxial trichomes were generated prematurely, the leaf blades elongated, and the blade base angles enlarged, as is observed for adult leaves. In hyl1-2 p35S::miR156a and hyl1-2 spl9-4 spl15-1 plants, increased accumulation of miR156a and repressed expression of the SPL genes were concomitant with a complete or partial rescue of the hyl1-2 phenotype in phase defects. In contrast, overexpression of the SPL9 gene in hyl1-2 mutants led to total disappearance of the juvenile phase. Moreover, HYL1 prevented the premature accumulation of adult-related transcripts in the primary leaves. Taken together, these results suggest that HYL1 controls the expression levels of miR156-targeted SPL genes and enables plants to undergo the juvenile phase, an important and critical step during plant development to ensure maximum growth and productivity.

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“…Traditionally, this period is divided into four stages: seedling, rosette, folding and heading, which are characterized by the morphological features of leaves (He et al ., ; Yu et al ., ). Primary leaves grow at the seedling stage; rosette leaves assist head formation by supplying photosynthates to head leaves; the folding leaves are curved inward to establish a posture for head formation and to provide a shade to the head; and the heading leaves around the shoot apexes, which are incurved enough to form a compact head and thus become the nutrient storage organ (He et al ., ; Opeña, 1984 ). Many factors such as temperature, light intensity, auxin concentration and ratio of carbohydrate to nitrogen may affect the formation of the leafy head (Ito and Kato, ).…”

Section: Introductionmentioning

confidence: 98%

BrpSPL9 (Brassica rapa ssp. pekinensis SPL9) controls the earliness of heading time in Chinese cabbage

Wang

Bai

et al. 2013

Plant Biotechnology Journal

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SummaryThe leafy heads of cabbage (Brassica oleracea), Chinese cabbage (Brassica rapa ssp. pekinensis), Brussels sprouts (B. oleracea ssp. gemmifera) and lettuce (Lactuca sativa) comprise extremely incurved leaves that are edible vegetable products. The heading time is important for high quality and yield of these crops. Here, we report that BrpSPL9-2 (B. rapa ssp. pekinensis SQUAMOSA PROMOTER BINDING-LIKE 9-2), a target gene of microRNA brp-miR156, controls the heading time of Chinese cabbage. Quantitative measurements of leaf shapes, sizes, colour and curvature indicated that heading is a late adult phase of vegetative growth. During the vegetative period, miR156 levels gradually decreased from the seedling stage to the heading one, whereas BrpSPL9-2 and BrpSPL15-1 mRNAs increased progressively and reached the highest levels at the heading stage. Overexpression of a mutated miR156-resistant form of BrpSPL9-2 caused the significant earliness of heading, concurrent with shortening of the seedling and rosette stages. By contrast, overexpression of miR156 delayed the folding time, concomitant with prolongation of the seedling and rosette stages. Morphological analysis reveals that the significant earliness of heading in the transgenic plants overexpressing BrpSPL9-2 gene was produced because the juvenile phase was absent and the early adult phase shortened, whereas the significant delay of folding in the transgenic plants overexpressing Brp-MIR156a was due to prolongation of the juvenile and early adult phases. Thus, miR156 and BrpSPL9 genes are potentially important for genetic improvement of earliness of Chinese cabbage and other crops.

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Leafy head formation of the progenies of transgenic plants of Chinese cabbage with exogenous auxin genes

Cited by 55 publications

References 9 publications

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

HYL1 controls the miR156-mediated juvenile phase of vegetative growth

BrpSPL9 (Brassica rapa ssp. pekinensis SPL9) controls the earliness of heading time in Chinese cabbage

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