Genetic features of Patanwadi, Marwari and Dumba sheep breeds (India) inferred by microsatellite markers

Jyotsana, Basanti; Jakhesara, Subhash J.; Prakash, Ved; Rank, DN; Vataliya, P. H.

doi:10.1016/j.smallrumres.2010.03.008

Cited by 18 publications

(13 citation statements)

References 13 publications

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“…Obtained molecular genetic parameters (Na, Ne, PIC and Hª) obtained from this study were higher than previous research (Farid et al 2000;Arranz et al 2001;Santos-Silva et al 2008;Jyotsana et al 2010;Al-Barzinji et al 2011;Hoda & Marsan 2012;Gaouar et al 2014Gaouar et al , 2015bGaouar et al , 2016aGaouar et al , 2016bYilmaz et al 2014;Kdidi et al 2015;Othman et al 2016). These results indicated that microsatellites used in the present study have a high confidence to reveal genetic diversity for these breeds.…”

Section: Discussioncontrasting

confidence: 73%

New genetic identification and characterisation of 12 Algerian sheep breeds by microsatellite markers

Ameur

Ata

Benyoucef

et al. 2017

Italian Journal of Animal Science

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Exposition of variations between breeds is very important for genetic diversity. Determination of this variation is needed to reveal population structure and relationship between populations and planning national breeding and conservation programmes. This study was carried out in 296 animals from 12 different local sheep breeds (Barbarine, Ouled Djellal, Ifilene, Srandi, Darâa, Rembi, Berbere, Taâdmit, Hamra, Sidaou, Tazegzawt and D'men) reared in different regions of Algeria. Fifteen microsatellite markers were used to determine between breed genetic diversity. The population of 12 sheep breeds studied from Algeria exhibited a high number of alleles (24.67) and polymorphic information content (0.90). Observed heterozygosity values were lower than expected for all molecular markers except INRA0123 locus. Obtained G ST value from the present study indicated that 1.9% of total genetic variation resulted from the differences between the breeds. The present study supplied important information to understand between breed genetic differences. Moreover, it has provided the opportunity to discuss with previously reported results. In light of these findings, it can be said that studied microsatellite markers can be successfully used to determine genetic diversity and population structure in Algerian sheep breeds. ARTICLE HISTORY

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Section: Discussioncontrasting

confidence: 73%

New genetic identification and characterisation of 12 Algerian sheep breeds by microsatellite markers

Ameur

Ata

Benyoucef

et al. 2017

Italian Journal of Animal Science

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“…The total number of alleles (216) observed in the present study on Nellore Brown sheep were higher than those reported by Jandruvo et al, 2005 in Czech and Slovak Republic sheep (143), Arora and Bhatia. 2006 in Magra sheep (144), Sodhi et al, 2006 in Nali and Chokla sheep (159), Arora et al, 2008 in Jaluani sheep (148), Kumarasamy et al, 2009 in Coimbatore sheep (143), Pramod et al, 2009 The allele size range (75 to 297 bp) measured in the present study were comparable to allele sizes reported by Jandruvo et al (2005) in Czech and Slovak Republic sheep, Sharma et al (2006) in Hassan sheep, Sodhi et al (2006) in Nali and Chokla sheep, Mukesh et al (2006) in Nali, Chokla and Garole sheep, Girish et al (2007) in Nilgiri sheep, Qanbari et al (2007) in Afshari sheep, Prema et al (2008a,b) in Mecheri and Madras Red sheep, Lasagna et al (2009) in Appenninica sheep, Pramod et al (2009) in Vembur sheep, Kumarasamy et al (2009) in Coimbatore sheep, Sharma et al (2010) The mean number of observed alleles (9.0 ± 1.83) estimated in the present study were in conformity with the earlier reports of Ivanikovic et al (2005) in Pag island sheep, Ligda et al (2009) in Greek sheep breeds, Jyotsana et al (2010) in Patanwadi and Marwari breeds, but, higher than the findings of Arora and Bhatia. (2004) in Magra sheep, in Nali and Chokla breeds, Qanbari et al (2007) in Afshari sheep, Arora et al (2008) in Jalauni sheep, Girish et al (2007) in Nilagiri sheep, Nahas et al (2008) in Egyptian breeds, Vani (2012) and Surekha (2015) in Nellore Jodipi sheep and lower than the findings of Crispim et al (2014) in Pantaneiro sheep, Sassidy-Zaidy et al (2014) in Barbarine sheep and Hoda et al (2014) in Albanian sheep.…”

Section: Oarvh72supporting

confidence: 91%

“…The studies on Patanwadi, Marwari and Dumba breeds (Jyotsana et al, 2010) using 20 microsatellite markers revealed a total of 207 alleles across the pooled population with mean number of alleles being 10.35. A total of 165 alleles were found in Patanwadi sheep, 181 in Marwari and 160 in Dumba sheep and the mean number of alleles observed were 8.25, 9.05 and 8.00 in Patanwadi, Marwari and Dumba breeds, respectively.…”

Section: Indigenous Breedsmentioning

confidence: 99%

“…The highest average observed heterozygosity (H o ) was seen in Marwari (0.6380) followed by Dumba (0.6360) and Patanwadi (0.6058) with an overall mean of 0.62. The expected heterozygosity values (H e ) were 0.70, 0.73 and 0.69 in Patanwadi, Marwari and Dumba breeds, respectively with an overall mean of 0.70 (Jyotsana et al, 2010). Pandey et al (2010) reported that the average observed heterozygosity was 0.501, while the average expected heterozygosity varied from 0.354 TGLA377 The observed heterozygosity values ranged from 0.180 (CSSM47) to 0.881 (OarFCB48) with a mean value of 0.618, while the expected heterozygosity values ranged from 0.349 (BM8125) to 0.865 (OMHC1) with a mean value of 0.725 in Kilakarsal sheep (Radha et al, 2011).…”

Section: Indigenous Breedsmentioning

confidence: 99%

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Studies on Genetic Variability in Nellore Brown Sheep Using Microsatellite Markers

Jonnakutti¹,

Bhupathi²,

Ekambaram³

et al. 2018

Int. J. Livest. Res.

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Nellore sheep is the tallest mutton breed of India and its home tract is Nellore and Prakasam districts of Andhra Pradesh, but widely distributed throughout the state of Andhra Pradesh and parts of Telangana. Three varieties are distinguished phenotypically based on coat colour as Palla (completely white), Jodipi (white with black patches) and Dora/Brown (completely brown). Nellore Brown sheep are predominantly present in Rayalseema region (Kadapa, Kurnool, Anantapur districts) of Andhra Pradesh. This breed is well known for its disease resistance and thrives well in harsh climatic conditions of Rayalseema. The assessment of genetic variability is important to monitor the gene flow in populations, for conservation of breed and also to determine the level of inbreeding in the population. The present study was undertaken to evaluate the genetic variability present in Nellore Brown sheep population by using twenty four microsatellite markers from the panel of markers recommended by ISAG-FAO for the genetic diversity studies in sheep. A total of fifty blood samples were collected at random from unrelated Nellore Brown animals maintained by farmers in its breeding tract. Phenol-chloroform method was used for isolation of DNA from blood samples. The quality and quantity of isolated DNA was checked using gel electrophoresis and Nanodrop spectrophotometer, respectively. The PCR products were resolved using agarose gel electrophoresis. The allele size for each locus was scored manually from the electrophoresed gels. The allele frequencies, observed number of alleles (N a), effective number of alleles (N e), observed (H o) and expected (H e) heterozygosity, within population inbreeding estimates (F IS) were computed using POPGENE version 1.32 software. A total of 216 alleles were identified across all the twenty four microsatellite loci studied. The number of alleles at each locus varied from a minimum of four (HUJ616) to a maximum of thirteen (OarCP49) with a mean of 9.0 alleles across all loci. Allele size ranged from a minimum of 75 bp (OarCP49) to a maximum of 297 bp (HSC), while allele frequencies ranged from 0.0102 to 0.3980. The most frequent allele number was nine. The number of effective alleles ranged from 3.8757 (HUJ616) to 9.6232 (OarCP49). The mean number of effective alleles with an overall mean of 6.78 ± 1.79 alleles. The observed heterozygosity ranged from 0.0408 (BM1314) to 0.1429 (OarHH35, OarHH41) with a mean value of 0.0985 ± 0.025, whereas, the expected heterozygosity ranged from 0.7420 (HUJ616) to 0.8961(OarCP49) with a mean value of 0.8409 ± 0.046. All the twenty four microsatellite loci (100 percent) were found to be highly polymorphic and the PIC values ranged from 0.6945 (HUJ616) to 0.8961 (OarCP49) with a mean PIC value of 0.8240 ± 0.061. The inbreeding estimates obtained in this study were all positive and varied from 0.8318 (OarHH35) to 0.9534 (BM1314). The mean F IS value of 0.8825 ± 0.03 indicated the deficiency of heterozygotes. The Chi-square test revealed that all the twenty four loci were...

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“…The product size (bp) varied from 71 for OARCP20 to 288 for ILSTS11 in both the breeds. From the literature studied, Arora and Bhatia (2004, Farid et al(2000), Girish et al (2007), Nanekarani et al (2010) and Prema et al (2008aPrema et al ( , 2008b reported a lower mean number of alleles while the authors Arora et al (2010), Chen et al (2009), Diez-Tascon et al (2000, Jyotsana et al (2010), Ozerov et al (2008), Radha et al (2011) andRodrigo et al (2010) reported higher number of alleles than the present findings. The differences in allele number and size may be attributable to the study of unrelated local populations spread over distant geographical area, which harboured high degree of genetic variation.…”

Section: Resultssupporting

confidence: 40%

Molecular genetic studies on Nellore and Deccani sheep using microsatellite markers

Amareswari

Prakash

Ekambaram

et al. 2017

IJAR

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The study was undertaken to understand the genetic architecture of Deccani and Nellore sheep breeds and to assess the genetic distance between the breeds. Allele diversity, genetic variability and population structure in the two breeds were estimated using 30 microsatellite markers. A total of 100 sheep, 50 each from Deccani and Nellore breeds were genotyped. The total number of alleles observed was 254 and 260 in Deccani and Nellore, respectively. The mean expected heterozygosity among the breeds ranged from 0.79 (Deccani) to 0.80 (Nellore) whereas the mean observed heterozygosity levels ranged from 0.77 in Deccani to 0.78 in Nellore sheep. The overall mean inbreeding coefficient was 0.029 in Deccani and 0.031 in Nellore sheep and of the 30 loci studied, 14 loci in Deccani and 16 loci in Nellore sheep showed negative inbreeding coefficient indicating the presence of outbreeding. However, eight loci (BM1314, BM6506, BM8125, ILSTS28, MAF70, MCM140, OarCB226 and OarFCB128) showed positive, mild to moderate inbreeding ranging from 0.007 to 0.25 in Deccani and 0.066 to 0.37 in Nellore sheep studied. The F IS values ranged from -0.372 to 0.74, F ST values ranged from 0.001 to 0.172 and F IT values ranged from -0.370 to 0.728 between the Deccani and Nellore sheep breeds studied. The PIC values ranged from 0.498 to 0.886 with a mean of 0.773 in Deccani and from 0.574 to 0.894 with a mean of 0.777 in Nellore breed. The information generated from the present study will be valuable for setting conservation priority of these two breeds.

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Genetic features of Patanwadi, Marwari and Dumba sheep breeds (India) inferred by microsatellite markers

Cited by 18 publications

References 13 publications

New genetic identification and characterisation of 12 Algerian sheep breeds by microsatellite markers

New genetic identification and characterisation of 12 Algerian sheep breeds by microsatellite markers

Studies on Genetic Variability in Nellore Brown Sheep Using Microsatellite Markers

Molecular genetic studies on Nellore and Deccani sheep using microsatellite markers

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