2001
DOI: 10.1073/pnas.141113098
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Genetic fidelity under harsh conditions: Analysis of spontaneous mutation in the thermoacidophilic archaeon Sulfolobus acidocaldarius

Abstract: Microbes whose genomes are encoded by DNA and for which adequate information is available display similar genomic mutation rates (average 0.0034 mutations per chromosome replication, range 0.0025 to 0.0046). However, this value currently is based on only a few well characterized microbes reproducing within a narrow range of environmental conditions. In particular, no genomic mutation rate has been determined either for a microbe whose natural growth conditions may extensively damage DNA or for any member of th… Show more

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Cited by 151 publications
(183 citation statements)
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“…For example, the 44-bp deletion had no recognizable direct repeats, inverted repeats, or secondary DNA structure at the endpoints, consistent with the deletions observed in S. acidocaldarius (12). Moreover, we observed two Ϯ1-bp mutations in runs of single bases (T 3 and A 6 in strains P2A-045 and -139, respectively) ( Table 2), although there was no strong preference for Ϫ1 bp mutations in long runs of the same base, as occurs in S. acidocaldarius, which is richer in homopolynucleotide sequences (11). However, in contrast to the pyrE bias observed for mutations in the pyrEF region of S. acidocaldarius (11), mutations were distributed fairly evenly throughout the region (Table 2).…”
Section: Discussionsupporting
confidence: 84%
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“…For example, the 44-bp deletion had no recognizable direct repeats, inverted repeats, or secondary DNA structure at the endpoints, consistent with the deletions observed in S. acidocaldarius (12). Moreover, we observed two Ϯ1-bp mutations in runs of single bases (T 3 and A 6 in strains P2A-045 and -139, respectively) ( Table 2), although there was no strong preference for Ϫ1 bp mutations in long runs of the same base, as occurs in S. acidocaldarius, which is richer in homopolynucleotide sequences (11). However, in contrast to the pyrE bias observed for mutations in the pyrEF region of S. acidocaldarius (11), mutations were distributed fairly evenly throughout the region (Table 2).…”
Section: Discussionsupporting
confidence: 84%
“…Moreover, we observed two Ϯ1-bp mutations in runs of single bases (T 3 and A 6 in strains P2A-045 and -139, respectively) ( Table 2), although there was no strong preference for Ϫ1 bp mutations in long runs of the same base, as occurs in S. acidocaldarius, which is richer in homopolynucleotide sequences (11). However, in contrast to the pyrE bias observed for mutations in the pyrEF region of S. acidocaldarius (11), mutations were distributed fairly evenly throughout the region (Table 2). , and the lower part shows the changes found in strain P2A-003, where SSO0620 is partitioned and the arrow on the boxed ISC1359 indicates the direction of the transpose gene.…”
Section: Discussionmentioning
confidence: 80%
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“…S2, ranging from 910,000 (Ϯ90,000) years ago for the North American and Mutnovsky populations to 140,000 (Ϯ25,000) years ago for the M.16.27 and M.14.25 divergence. Based on these dates, the average rate of nucleotide substitution was estimated to be 4.66 ϫ 10 Ϫ9 (Ϯ6.76 ϫ 10 Ϫ10 ) substitutions per site per year (Table S4), on the order of the universal rates estimated from comparisons of other species (46,47,63) and predicted from laboratory determinations of mutation rates in other Sulfolobus species (64). This unique method for dating microbial populations based on constraining colonization dates with geological events allows estimation of absolute rates of genome dynamics on more recent time scales than have previously been examined.…”
Section: Biogeography Of the Variable Genomementioning
confidence: 99%