Genetic Fusarium chemotyping as a useful tool for predicting nivalenol contamination in winter wheat

Pasquali, Matias; Giraud, Frédéric; Brochot, Céline; Cocco, Emmanuelle; Hoffmann, Lucien; Bohn, Torsten

doi:10.1016/j.ijfoodmicro.2009.11.009

Cited by 80 publications

(67 citation statements)

References 54 publications

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“…As reported elsewhere and in the current survey, the presence of more than one chemotype of F. culmorum was detected in the same field, perhaps a common finding (Jennings et al, 2004;Pasquali et al, 2010;Boutigny et al, 2014) that suggests that chemotypes may coexist without strongly interfering with each other.…”

Section: Discussionsupporting

confidence: 86%

Fusarium species and chemotypes associated with fusarium head blight and fusarium root rot on wheat in Sardinia

et al. 2015

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Environmental conditions in Sardinia (Tyrrhenian Islands) are conducive to fusarium root rot (FRR) and fusarium head blight (FHB). A monitoring survey on wheat was carried out from 2001 to 2013, investigating relations among these diseases and their causal agents. FHB was more frequently encountered in the most recent years while FRR was constantly present throughout the monitored period. By assessing the population composition of the causal agents as well as their genetic chemotypes and EF-1a polymorphisms, the study examined whether the two diseases could be differentially associated to a species or a population. Fusarium culmorum chemotypes caused both diseases and were detected at different abundances (88% 3-ADON, 12% NIV). Fusarium graminearum (15-ADON genetic chemotype) appeared only recently (2013) and in few areas as the causal agent of FHB. In F. culmorum, two haplotypes were identified based on an SNP mutation located 34 bp after the first exon of the EF-1a partial sequence (60% adenine, 40% thymine); the two populations did not segregate with the chemotype but the A-haplotype was significantly associated with FRR in the Sardinian data set (P = 0Á001), suggesting a possible fitness advantage of the A-haplotype in the establishment of FRR that was neither dependent on the sampling location nor the sampling year. The SNP determining the Sardinian haplotype is distributed worldwide. The question whether the A-haplotype segregates with characters facilitating FRR establishment will require further validation on a specifically sampled international data set.

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Section: Discussionsupporting

confidence: 86%

Fusarium species and chemotypes associated with fusarium head blight and fusarium root rot on wheat in Sardinia

et al. 2015

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“…The 3- isolates belonging to population 2 were included in the analysis of aggressiveness, ten of these were included in the growth rate analysis c 17 3-ADON isolates were included in the analysis of aggressiveness, 16 of these were included in the growth rate analysis ADON chemotype also predominated among a small set of FGSC isolates from an earlier collection of Norwegian cereals (Langseth et al 1999(Langseth et al , 2001, and was found in oat and wheat samples collected between 2009 and 2011 in Sweden, whereas the 15-ADON chemotype was not observed (Fredlund et al 2013;Lindblad et al 2013 (Nielsen et al 2012). Our data add to a growing set of studies indicating a geographical partitioning of trichothecene types (chemotypes or genotypes) throughout Europe, with 3-ADON dominating in Northern Europe, whereas 15-ADON appears predominant in the Western and Southern Europe including the UK (Jennings et al 2004), Belgium (Audenaert et al 2009), Italy (Prodi et al 2009;Somma et al 2014), Luxembourg (Pasquali et al 2010), Poland (Stępień et al 2008), Hungary (Toth et al 2005), Germany (Talas et al 2011), and France (Boutigny et al 2014). …”

Section: Discussionsupporting

confidence: 51%

Genetic and phenotypic diversity within the Fusarium graminearum species complex in Norway

et al. 2015

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As has been observed in several European countries, the frequency of Fusarium head blight (FHB) caused by members of the Fusarium graminearum species complex (FGSC) has increased in Norwegian cereals in recent years, resulting in elevated levels of deoxynivalenol in cereal grains. The objective of this study was to determine if this increase was associated with changes in FGSC composition within Norway. FGSC isolates collected from wheat, oats and barley in Norway during two periods, mainly 1993-1998 and 2004-2007, were characterized to determine species and trichothecene genotype composition and to assess levels of genetic variation and population structure. In vitro growth rates at different temperatures and aggressiveness in spring wheat were further characterized for a sub-selection of isolates. All Norwegian isolates were identified as F. graminearum. The 3-acetyl-deoxynivalenol (3-ADON) trichothecene type was dominant. However, isolates with the 15-ADON chemotype were detected in Norway for the first time and may represent a recent introduction of this trichothecene type. Bayesian-model based clustering and analyses of genetic differentiation indicated the persistence over the last 20 years of two sympatric and partially admixed populations of F. graminearum in Norway. Significant differences in average in vitro growth rates and aggressiveness were observed between these two populations. Our results demonstrate that the recent increase in prevalence of the FGSC in Norwegian cereals do not correspond to any dramatic changes in FGSC species or trichothecene chemotype composition. However, significant changes in population frequencies were observed among Norwegian F. graminearum.

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“…In particular, Phusion Taq mix (Finzyme, Espoo, Finlande) and 500 mM of each primer in a total volume of 10 ml were used to distinguish F. poae, F. culmorum, F. graminearum and F. avenaceum (Pasquali et al, 2010). Due to the close genetic relationship to F. poae, we also investigated the presence of F. langsethiae using specific primers defined by Wilson et al (2004).…”

Section: Molecular Identification Of Fusarium Strainsmentioning

confidence: 99%

Fusarium head blight and associated mycotoxin occurrence on winter wheat in Luxembourg in 2007/2008

Giraud¹,

Pasquali²,

Jarroudi

et al. 2010

Food Additives & Contaminants: Part A

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Genetic Fusarium chemotyping as a useful tool for predicting nivalenol contamination in winter wheat

Cited by 80 publications

References 54 publications

Fusarium species and chemotypes associated with fusarium head blight and fusarium root rot on wheat in Sardinia

Fusarium species and chemotypes associated with fusarium head blight and fusarium root rot on wheat in Sardinia

Genetic and phenotypic diversity within the Fusarium graminearum species complex in Norway

Fusarium head blight and associated mycotoxin occurrence on winter wheat in Luxembourg in 2007/2008

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