Genetic heterogeneity of L-Zagreb mumps virus vaccine strain

Košutić-Gulija, Tanja; Forčić, Dubravko; Šantak, Maja; Ramljak, Ana; Mateljak-Lukacevic, Sanja; Mažuran, Renata

doi:10.1186/1743-422x-5-79

Cited by 16 publications

(12 citation statements)

References 20 publications

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“…cDNA was prepared from total RNA using random hexamers and MuLV reverse transcriptase as reported previously (Košutić -Gulija et al, 2008). Nested PCRs were performed for HN, F and P genes.…”

Section: Methodsmentioning

confidence: 99%

A study of genetic variability of human parainfluenza virus type 1 in Croatia, 2011–2014

Košutić-Gulija

Slović

Ljubin‐Sternak

et al. 2016

Journal of Medical Microbiology

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Section: Methodsmentioning

confidence: 99%

A study of genetic variability of human parainfluenza virus type 1 in Croatia, 2011–2014

Košutić-Gulija

Slović

Ljubin‐Sternak

et al. 2016

Journal of Medical Microbiology

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“…Further studies based on reverse genetics are required to investigate the relationship between these diversities and the molecular mechanism of attenuation and/or virulence. In spite of the fact that genetic heterogeneity in other mumps vaccine strains has also been observed (Boriskin et al, 1992;Amexis et al, 2002;Sauder et al, 2006;Kosutic-Gulija et al, 2008;Tillieux et al, 2009), it might be necessary to prepare a cloned attenuated vaccine RS-12 virus as a genetically homogenous seed. There is currently limited data available on sequencing of the full-length genomes of attenuated MuV vaccine strains.…”

Section: Resultsmentioning

confidence: 99%

Wild and attenuated vaccine RS-12 strains of mumps virus exhibit differences in amino acid sequences of their proteins

Alirezaie¹,

Shahbazi²,

Ss³

et al. 2014

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Summary. -Attenuated mumps virus (MuV) RS-12 strain-based vaccine is one of several effective vaccines available in the prevention of mumps. Since previous studies have unveiled only about one-third of the attenuated vaccine RS-12 strain genome sequence, the rest of sequence and molecular basis for attenuation remained unsolved. Therefore, in this study, the full-length genome sequences of wild and attenuated RS-12 strains were determined and compared. The comparison revealed nucleotide substitutions at 9 positions leading to amino acid substitutions at 6 positions in P, V, I, M, and L proteins, while the remaining substitutions were silent. This result indicates that the observed mutations in P, V, I, M, and L proteins of MuV might be responsible for the attenuation of the RS-12 vaccine strain.

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“…L-Zagreb variants A and B were plaque purified and their genomes were completely sequenced [13]. Genomes of the two variants differ in nt G1073T, C5261T and G11345T, the first letter indicating the nt in variant A cDNA and the second in variant B cDNA, respectively.…”

Section: Viral Materialsmentioning

confidence: 99%

“…Sequences spanning nt site 5261 from both plaque purified viral variants were PCR amplified and inserted into pUC19 plasmid (New England Biolabs, USA), thus obtaining plasmid variants A and B (PVA and PVB, respectively) [13]. PVA had a cytidine (C) at position 5261 of the mumps cDNA and PVB had a thymidine (T).…”

Section: Plasmid Materialsmentioning

confidence: 99%

“…As an RNA viral population, the mumps virus strain L-Zagreb was used. Using the method of cloning and sequencing of the entire mumps genome, we previously determined that L-Zagreb is a heterogeneous mixture of numerous variants [13]. The genome of the most abundant minor variant (variant B) differs from the sequence of the major variant (variant A) at three nt positions [13].…”

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of CE‐SSCP to standard RFLP‐CE‐FLA method in quantification of known viral variants within an RNA virus quasispecies

et al. 2011

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RNA viruses display the highest replication error rate in our biosphere, leading to highly diverse viral populations termed quasispecies. The gold standard method for detection and quantification of variants in a quasispecies is cloning and sequencing, but it is expensive, laborious and time consuming. Therefore, other mutation detection approaches, including SSCP, are often used. In this study, we demonstrate development and the usage of a CE-SSCP method for quantification of two nearly identical viral variants in heterogenic population of a mumps virus strain and its comparison to RFLP-CE-fragment length analysis (RFLP-CE-FLA). Analyzed PCR fragments were of the same size (245 bp) with one difference in their nucleotide sequence. The limit of detection of both methods was at 5% of the minor variant. When PCR amplicons of the two variants were pooled, methods' results were very similar. On the contrary, the quantification results of samples in which variants were mixed prior to PCR showed substantial difference between the two methods. Our results indicate that although both methods can be used for detection and monitoring of a specific mutation within a viral population, caution should be taken when quantitative analysis of complex samples is based solely on results of one method.

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Genetic heterogeneity of L-Zagreb mumps virus vaccine strain

Abstract: Background: The most often used mumps vaccine strains Jeryl Lynn (JL), RIT4385, Urabe-AM9, L-Zagreb and L-3 differ in immunogenicity and reactogenicity. Previous analyses showed that JL, Urabe-AM9 and L-3 are genetically heterogeneous.

Cited by 16 publications

References 20 publications

A study of genetic variability of human parainfluenza virus type 1 in Croatia, 2011–2014

A study of genetic variability of human parainfluenza virus type 1 in Croatia, 2011–2014

Wild and attenuated vaccine RS-12 strains of mumps virus exhibit differences in amino acid sequences of their proteins

Comparative analysis of CE‐SSCP to standard RFLP‐CE‐FLA method in quantification of known viral variants within an RNA virus quasispecies

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