Genetic, historical and linguistic perspectives on the origin of the Kelantanese Malays

Wei, Loo Keat; Gan, Siew Hua

doi:10.1016/j.gene.2014.05.016

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…The lower T allele frequency can be attributed to the fact that this study population was composed entirely of females, and all subjects were selected from the HUSM. However, Kelantanese Malay residing in the remote northeastern regions of the Malaysia are believed to have a unique genetic signature (Loo and Gan, 2014). Similarly, the T allele among Malaysian Chinese (14%) (Choo et al, 2011) is much higher compared with the Kelantanese Malay.…”

Section: Discussionmentioning

confidence: 99%

Development of a multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677C>T and TNF-α -308G>A variants in a Malay population

Shaik

Tan

Abubakar

et al. 2018

APJMBB

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Methylenetetrahydrofolate reductase (MTHFR) and tumor necrosis factor-alpha (TNF-α) play major roles in cardiovascular and inflammatory disorders. This study aimed to develop a new multiplex PCR genotyping method for the simultaneous detection of MTHFR 677C>T and TNF-α -308G>A, which are the two single nucleotide polymorphisms (SNPs) that are widely known to confer susceptibility to major vascular and inflammatory disorders. DNA was amplified using multiplex PCR, which was optimized by evaluations of the annealing temperature, the effects of various magnesium chloride, primer and enzyme concentrations, and the amount of DNA template. Restriction fragment length polymorphism (RFLP) analysis was performed in two separate tubes followed by agarose gel electrophoresis. One hundred twenty-nine healthy volunteers were recruited, and the MTHFR 677C>T and TNF-α -308G>A variants were genotyped using a novel multiplex PCR-RFLP technique. The results were confirmed by DNA sequencing. The allele frequencies of MTHFR 677C>T were 97.29% (C allele) and 2.71% (T allele). For TNF-α -308G>A, the allele frequencies were 98.45% (G allele) and 1.55% (A allele). The PCR-RFLP method developed in this study is simple, cost-effective and time-saving. It can be used to simultaneously genotype subjects for the MTHFR 677C>T and TNF-α -308G>A variants with 100% concordance with DNA sequencing data. This method can be routinely used for rapid investigation of the MTHFR 677C>T and TNF-α -308G>A variants.

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Section: Discussionmentioning

confidence: 99%

Development of a multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677C>T and TNF-α -308G>A variants in a Malay population

Shaik

Tan

Abubakar

et al. 2018

APJMBB

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Signaling pathway genes for blood pressure, folate and cholesterol levels among hypertensives: an epistasis analysis

et al. 2014

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Irregular atrial pressure, defective folate and cholesterol metabolism contribute to the pathogenesis of hypertension. However, little is known about the combined roles of the methylenetetrahydrofolate reductase (MTHFR), apolipoprotein-E (ApoE) and angiotensin-converting enzyme (ACE) genes, which are involved in metabolism and homeostasis. The objective of this study is to investigate the association of the MTHFR 677 C>T and 1298A>C, ACE insertion-deletion (I/D) and ApoE genetic polymorphisms with hypertension and to further explore the epistasis interactions that are involved in these mechanisms. A total of 594 subjects, including 348 normotensive and 246 hypertensive ischemic stroke subjects were recruited. The MTHFR 677 C>T and 1298A>C, ACE I/D and ApoEpolymorphisms were genotyped and the epistasis interaction were analyzed. The MTHFR 677 C>T and ApoE polymorphisms demonstrated significant associations with susceptibility to hypertension in multiple logistic regression models, multifactor dimensionality reduction and a classification and regression tree. In addition, the logistic regression model demonstrated that significant interactions between the ApoE E3E3, E2E4, E2E2 and MTHFR 677 C>T polymorphisms existed. In conclusion, the results of this epistasis study indicated significant association between the ApoE and MTHFR polymorphisms and hypertension.

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Influence of TNF-α and ESR1 Polymorphisms on Vascular, Hormonal and Inflammatory Biomarkers in Migraine

Shaik

Abubakar

Tan

et al. 2018

J. of Medical Sciences

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Genetic, historical and linguistic perspectives on the origin of the Kelantanese Malays

Cited by 3 publications

References 15 publications

Development of a multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677C>T and TNF-α -308G>A variants in a Malay population

Development of a multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677C>T and TNF-α -308G>A variants in a Malay population

Signaling pathway genes for blood pressure, folate and cholesterol levels among hypertensives: an epistasis analysis

Influence of TNF-α and ESR1 Polymorphisms on Vascular, Hormonal and Inflammatory Biomarkers in Migraine

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