Genetic influences on dental enamel that impact caries differ between the primary and permanent dentitions

Abstract

Clinically, primary and permanent teeth are distinct anatomically and the presentation of caries lesions differs between the two dentitions. However, the possibility exists that genetic contributions to tooth formation of the two dentitions are different. The purpose of this study was to test the hypothesis that genetic associations with an artificial caries model will not be the same between primary and permanent dentitions. Enamel samples from primary and permanent teeth were tested for microhardness at baseline, after carious lesion creation, and after fluoride application to verify association with genetic variants of selected genes. Associations were found between genetic variants of ameloblastin, amelogenin, enamelin, tuftelin, tuftelin interactive protein 11, and matrix metalloproteinase 20 and enamel from permanent teeth but not with enamel from primary teeth. In conclusion, our data continue to support that genetic variation may impact enamel development and consequently individual caries susceptibility. These effects may be distinct between primary and permanent dentitions.

Interaction of lifestyle, behaviour or systemic diseases with dental caries and periodontal diseases: consensus report of group 2 of the joint EFP/ORCA workshop on the boundaries between caries and periodontal diseases

“…Some also appear to be dentition specific, which likely reflects the known anatomical/structural differences between both dentitions (Bayram et al 2015).…”

Genes Involved in the Enamel Development Are Associated with Calcium and Phosphorus Level in Saliva

“…Some of our previous studies investigated the association between these genes and caries susceptibility and enamel alteration [Deeley et al, 2008; Tannure et al, 2012; Shimizu et al, 2012; Jeremias et al, 2013; Abbasoğlu et al, 2015; Bayram et al, 2015]. TUFT1 and TUIP11 were associated with variations in enamel microhardness [Shimizu et al, 2012].…”

“…ENAM were associated with enamel hypoplasia [Jeremias et al, 2013]. AMELX, TFIP11, ENAM, TUFT1, KLK4, AMBN and MMP20 also observed an association between some of these genes with caries susceptibility in primary and permanent dentition [Deeley et al, 2008; Tannure et al, 2012; Shimizu et al, 2012 Jeremias et al, 2013; Abbasoğlu et al, 2015; Bayram et al, 2015]. …”

Variants on chromosome 4q21 near PKD2 and SIBLINGs are associated with dental caries

“…This study benefited from a large sample size and by investigating caries in both primary and permanent dentitions, which previous work has suggested differ in their genetic risk 3, 36 . We, too, found different results between dentitions, although this could be explained by differences in power.…”

“…Genetic association was tested using linear regression under the additive genetic model while simultaneously adjusting for age, sex, and two principal components of ancestry (generated across all cohorts using principal components analysis of 2,663 SNPs in 71 genes of interest as well as 96 ancestry-informative SNPs specifically chosen for modeling ancestry). Analyses were performed separately in 13 age- (i.e., child vs. adult) and race-stratified samples; this decision was based on hypothesized differences in the genes underlying caries susceptibility in the primary and permanent dentitions 3, 36 as well as to guard against spurious results due to population structure. Stouffer's inverse-variance weighted method was used to combine p-values across stratified analyses in order to determine statistical significance of associations across samples.…”