Genetic instability in Drosophila melanogaster

Rasmuson, Bertil; Montell, Ingvar; Rasmuson, Åsa; Svahlin, Helena; Westerberg, B. M.

doi:10.1007/bf00272664

Cited by 17 publications

(7 citation statements)

References 9 publications

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“…Furthermore, the FB-NOF element was found to be associated with the white end of TE28, but in this case the site of insertion is more distal than in w'. In situ hybridization experiments also suggest that FB-NOF is associated with Tp w + IV (Rasmuson et al, 1980). Therefore, FB-NOF could represent the intact functional FB element.…”

Section: Discussionmentioning

confidence: 89%

“…The finding that FB elements are flanking the warst + -TE strongly suggests that they might be responsible for the mobility of this large segment of DNA. Therefore, it was of interest to see whether they are also associated with the independently isolated Tps derived from white-crimson (Green, 1969a(Green, , 1969b and the Tp w + IV transposon (Rasmuson et al, 1980). For this purpose, fragments containing the internal segment of FB-NOF (fragment B; Figure 8) and the repeat (fragment A) were subcloned in pBR322.…”

Section: Correlation Of Fb Sequences With Other Large Transposable Elmentioning

confidence: 99%

“…The same analysis was performed with the Tp w + IV transposon which has moved from the X to the 4th chromosome. The white gene in this new position gave rise to a mottled phenotype, which suggested that it might have moved into a heterochromatic region (Rasmuson et al, 1980). Hybridization with single copy white sequences placed the transposon close to the telomere of the 4th chromosome at 102F.…”

Section: Correlation Of Fb Sequences With Other Large Transposable Elmentioning

confidence: 99%

“…On the basis of the genetic data, Green postulated a 'controlling element' causing both the instability and the transposition. Another transposition of the white locus has been described by Rasmuson and collaborators (Rasmuson and Green, 1974;Rasmuson et al, 1980). The most extensively studied transposing element (TE) in Drosophila has been isolated and analyzed by G.Ising and his co-workers.…”

Section: Introductionmentioning

confidence: 98%

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Molecular analysis of large transposable elements carrying the white locus of Drosophila melanogaster

Paro

Goldberg²,

Gehring³

1983

The EMBO Journal

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A large transposable element (TE) comprising the white‐apricot and roughest genes has been found to transpose to well over a hundred sites scattered over the Drosophila genome. We report the cloning of the essential parts of several TEs. TE98 and TE28 sequences were cloned by ‘walking’ along the chromosome from the previously cloned heatshock genes. The ends of the TEs are characterized by dispersed repetitive elements belonging to the foldback (FB) family. FB elements are also associated with two independently isolated transposable elements originating from the white locus, Tp wc‐1 and Tp w+IV. The strong correlation between FB elements and large composite transposons suggests that a pair of these elements can mobilize large intermediary DNA segments. One particular FB family member, FB‐NOF, is associated with TE28, the white‐crimson (wc) mutant, the wc‐derived Tp wc‐1 and probably also with Tp w+IV. A unique sequence located close to the white end of TE28 was used to clone the borders of TE77 and the surrounding sequences in the bithorax region, indicating that the TE can be used as a probe for gene isolation. Some evolutionary implications of the large composite transposons are discussed.

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Section: Discussionmentioning

confidence: 89%

Section: Correlation Of Fb Sequences With Other Large Transposable Elmentioning

confidence: 99%

Section: Correlation Of Fb Sequences With Other Large Transposable Elmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Molecular analysis of large transposable elements carrying the white locus of Drosophila melanogaster

Paro

Goldberg²,

Gehring³

1983

The EMBO Journal

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“…3). Moreover, several of the alleles of the white locus are of particular interest because of their unusual genetic instability (4)(5)(6). Neither the gene product(s) of the white locus nor their mode of action have been determined.…”

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confidence: 99%

Physical map of the white locus of Drosophila melanogaster.

Levis¹,

Bingham²,

Rubin³

1982

Proc. Natl. Acad. Sci. U.S.A.

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The white locus of Drosophila melanogaster is a genetically well-characterized locus, mutations in which alter the degree or pattern of pigmentation of the eyes. Using a previously cloned DNA segment containing a portion of the white locus of a mutant allele, we have cloned and characterized the DNA of a 48-kilobase chromosomal region ofthe Canton S wild-type strain. We have mapped the positions, relative to restriction endonuclease cleavage sites, of several previously characterized chromosomal rearrangement breakpoints that bracket the white locus. These results define a segment of 14 kilobases that contains all of the white locus sequences necessary for the production of a wild-type eye color phenotype. By conventional criteria, no repetitive sequences are present within this 14-kilobase segment; however, we have identified an extremely weak DNA sequence homology between a portion of this segment and a chromosomal region in the vicinity of the zeste locus.The wl mutation at the white (w) locus was the first mutation reported in Drosophila melanogaster (1) We have previously isolated, by recombinant DNA techniques, a single-copy DNA segment from the white locus of a strain carrying the white-apricot (wa) allele (7). In this report, we describe the molecular cloning of overlapping segments of the Canton S wild-type white locus region. This has allowed us to construct a physical map ofrestriction enzyme cleavage sites in this 48-kilobase (kb) segment, to orient this physical map with respect to the genetic map, and to define a segment of 14 kb that contains all ofthe white locus sequences necessary to allow the production of a w+ (wild-type eye color) phenotype.MATERLALS AND METHODS Drosophila Strains and DNA Preparation. The Drosophila strains used have been described (7). DNA was prepared from adult flies that were anesthetized with C02, collected on ice, rapidly frozen, and stored at -700C. Flies were disrupted and a crude nuclear pellet was prepared as described (8) except that all steps subsequent to grinding were done on ice and Nitex 44 filters were used in place of milk filters. The nuclei were suspended in a small volume of homogenization buffer to which was added 10 vol of 30 mM Tris'HCl, pH 8.0/100 mM EDTA containing proteinase K at 0.5 mg/ml followed by N-lauroylsarcosine to 1%. After incubation overnight at 370C, the DNA was banded in CsCl gradients (9).Recombinant Phage and DNA Preparation. Phage from a Charon 4/Canton S D. melanogaster library (10) were screened by plaque hybridization (11) at a density of 2 x 104 plaques per 24 X 24 cm agar plate as described (7). For DNA preparation, phage were grown in 200 ml of liquid culture (7), debris was removed by low-speed centrifugation, and the phage were precipitated with polyethylene glycol (12), suspended in 2 ml of 10 mM Tris HCl, pH 7.5/10 mM MgSO4 containing 20 ,ug of DNase (Sigma), extracted with an equal volume of CHC13, and purified by two cycles ofCsCl step-gradient centrifugation (13). DNA was isolated from purified phage by phenol ...

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Genotoxicity studies with the unstable Zeste‐White (UZ) system of Drosophila melanogaster: Results with ten carcinogenic compounds

Batiste-Alentorn

Xamena

Creus

et al. 1991

Environ and Mol Mutagen

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To increase the number of chemicals tested using the zeste-white (UZ) somatic mutation assay, ten selected carcinogens (acetamide, acrylamide, benzo(alpha)pyrene, cyclophosphamide, diethylstilbestrol, 4-nitroquinoline N-oxide, propyleneimine, safrole, thiourea, and o-toluidine) have been evaluated in this assay. Our results show that all the compounds tested produce significant increases in the eye spot frequency at, at least, one of the concentrations assayed, indicating that the zeste-white assay appears to be highly sensitive to these carcinogenic compounds. That is in agreement with data previously reported by other authors.

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Genetic instability in Drosophila melanogaster

Cited by 17 publications

References 9 publications

Molecular analysis of large transposable elements carrying the white locus of Drosophila melanogaster

Molecular analysis of large transposable elements carrying the white locus of Drosophila melanogaster

Physical map of the white locus of Drosophila melanogaster.

Genotoxicity studies with the unstable Zeste‐White (UZ) system of Drosophila melanogaster: Results with ten carcinogenic compounds

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