Genetic Instability of an oligomycin resistanee mutation in yeast is associated with an amplification of a mitochondrial DNA segment

Ragnini-Wilson, Antonella; Fukuhara, Hiroshi

doi:10.1093/nar/17.17.6927

Cited by 9 publications

(5 citation statements)

References 25 publications

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“…jadinii (40 kbp), the la-gest linear molecules measured approximately 13 ,um, consistent with the restriction map size. Microheterogeneity of the terminal structure.…”

Section: Resultssupporting

confidence: 80%

“…When we examined by electron microscopy the preparations of the large mtDNA from W. mrakii (58 kbp long), we found only a single molecule (the VOL. 13,1993 pijperi mtDNA, used for enzymatic analyses, was spread by the procedure of Davis et al (3). The peak lengths are 22.02 ± 0.15 and 43.67 ± 0.39 kbp for supposed monomer and dimer molecules, respectively.…”

Section: Resultsmentioning

confidence: 99%

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Linear Mitochondrial DNAs of Yeasts: Frequency of Occurrence and General Features

Fukuhara

Sor

Drissi

et al. 1993

Molecular and Cellular Biology

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“…jadinii (40 kbp), the la-gest linear molecules measured approximately 13 ,um, consistent with the restriction map size. Microheterogeneity of the terminal structure.…”

Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Linear Mitochondrial DNAs of Yeasts: Frequency of Occurrence and General Features

Fukuhara

Sor

Drissi

et al. 1993

Molecular and Cellular Biology

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“…Cells were transformed into protoplasts by Zymolyase treatment, and crude mitochondria were prepared according to the conventional procedure used for Saccharomyces cerevisiae (2). The mtDNA was separated from the nuclear DNA by two cycles of isopycnic centrifugation in CsCl in the presence of bisbenzimide (13). Because of the high adeninethymine content, the mtDNA from all species examined could be efficiently separated from the nuclear DNA in the CsCl gradient.…”

Section: Methodsmentioning

confidence: 99%

“…The restriction maps presented in Fig. 2 13,1993 on May 10, 2018 by guest http://mcb.asm.org/ Downloaded from strain of the species, CBS 255, has a circular mtDNA with similar gene order (Fig. 2).…”

Section: Methodsmentioning

confidence: 99%

Linear mitochondrial DNAs of yeasts: frequency of occurrence and general features.

et al. 1993

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In most yeast species, the mitochondrial DNA (mtDNA) has been reported to be a circular molecule.However, two cases of linear mtDNA with specific termini have previously been described. We examined the frequency of occurrence of linear forms of mtDNA among yeasts by pulsed-field gel electrophoresis. Among the 58 species from the genera Pichia and Wiliopsis that we examined, linear mtDNA was found with unexpectedly high frequency. Thirteen species contained a linear mtDNA, as confirmed by restriction mapping, end labeling, and electron microscopy. The mtDNAs from Pichia pijperi, WiUliopsis mrakii, and P. jadinii were studied in detail. In each case, the left and right terminal fragments shared homologous sequences. Between the terminal repeats, the order of mitochondrial genes was the same in all of the linear mtDNAs examined, despite a large variation of the genome size. This constancy of gene order is in contrast with the great variation of gene arrangement in circular mitochondrial genomes of yeasts. The coding sequences determined on several genes were highly homologous to those of the circular mtDNAs, suggesting that these two forms of mtDNA are not of distant origins.Mitochondria have their own DNA which specifies several proteins of the respiratory complexes as well as mitochondrion-specific rRNAs and tRNAs. While the majority of these genomes seem to be circular, some organisms have been shown to have a linear mitochondrial DNA (mtDNA). Well-known examples are those of the ciliates of the genera Paramecium (7) and Tetrahymena (17). The first case of a linear mtDNA in yeasts was found in 1981 in Wlliopsis mrakii (formerly called Hansenula mrakii) (19). A second case was reported for a yeast identified as Candida parapsilosis (8; see also reference 1). Occurrence of linear DNAs in yeast mitochondria raises questions about their origin and mode of replication. In a number of other organelle genomes, linear forms of DNA have also been described, although in some cases, the reality of the linearity might be questioned because the terminal structure of these forms has not been defined. The evolutionary and taxonomical positions of these linear genomes with respect to the circular forms need to be clarified. These linear DNAs may replicate as a linear molecule, or they may replicate through circular intermediates. In this study, we examined (i) the frequency of the linear forms of mtDNAs among yeast species and (ii) whether they have any basic structures in common. In the accompanying report (4), we examine their possible modes of replication by analysis of their terminal structure. MATERIALS AND METHODSYeast strains. Yeast strains were obtained from Centraalbureau voor Schimmelcultures (CBS), Delft, The Netherlands. They are independent isolates of known origins. As a rule, the type strain of each species, as indicated in the CBS 1990 catalog, was chosen for analysis unless otherwise stated. The culture medium contained 1% yeast extract * Corresponding author.

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“…alterations in the sequences of the regulatory and coding regions of mtDNA in gliomas and the ontologically-early identification of amplification and heterogeneous areas of mtDNA amplification (suggesting deletions in the mitochondrial genome), have served to expand and extend early findings of abnormal mtDNA structure in cancer (13,14). The authors speculated on a possible association with petite mutants in yeast (15,16), where truncated mtDNA is associated with a dysfunction of the mitochondrion and with high degrees of amplification of the remaining DNA (see below).…”

Section: Alterations Of Mitochondrial Dnamentioning

confidence: 99%

The importance of mitochondria in the tumourigenic phenotype: Gliomas as the paradigm (Review)

Liang¹

2011

Int J Mol Med

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Abstract. Cancer arises from the accumulation of nuclear and cytoplasmic abnormalities, a phenomenon allowing for the expression of the tumourigenic phenotype. Gliomas represent the most frequently diagnosed tumours of the central nervous system in adults. Warburg hypothesized the importance of glycolysis in cancer cells, and implicated additional roles of mitochondria in neoplasia. Recent data have shown the importance of mitochondria in the tumourigenic phenotype, in particular, within the apoptotic process. There have been a variety of studies conducted on brain tumours revealing significant alterations of mitochondria within the tumourigenic phenotype. This review describes some of the more recent findings of mitochondria and gliomas, correlating findings to those observed in other cancers. Alterations in mitochondrial DNA copy number and location, as well as dependence of the cancer cell phenotype on mitochondria are emphasised. In addition to its role in apoptosis, the mitochondrion serves as an important element in the tumourigenic phenotype, and clinical approaches targeting this organelle have potential for the development of effective treatment regimens for patients with glioma and other neoplastic diseases.

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Genetic Instability of an oligomycin resistanee mutation in yeast is associated with an amplification of a mitochondrial DNA segment

Cited by 9 publications

References 25 publications

Linear Mitochondrial DNAs of Yeasts: Frequency of Occurrence and General Features

Linear Mitochondrial DNAs of Yeasts: Frequency of Occurrence and General Features

Linear mitochondrial DNAs of yeasts: frequency of occurrence and general features.

The importance of mitochondria in the tumourigenic phenotype: Gliomas as the paradigm (Review)

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