Genetic interaction of two abscisic acid signaling regulators, HY5 and FIERY1, in mediating lateral root formation

Chen, Hao; Xiong, Liming

doi:10.4161/psb.6.1.14231

Cited by 14 publications

(13 citation statements)

References 23 publications

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“…Conversely, on media supplemented with ABA, germination inhibition was significantly reduced in nf-yc triple mutants compared to Col-0 control plants (Figure 5B–C,J). This result was similar to previous observations of both abf1 or hy5 mutants [49], [50].…”

Section: Resultssupporting

confidence: 93%

NUCLEAR FACTOR Y Transcription Factors Have Both Opposing and Additive Roles in ABA-Mediated Seed Germination

et al. 2013

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In the model organism Arabidopsis thaliana the heterotrimeric transcription factor NUCLEAR FACTOR Y (NF-Y) has been shown to play multiple roles in facilitating plant growth and development. Although NF-Y itself represents a multi-protein transcriptional complex, recent studies have shown important interactions with other transcription factors, especially those in the bZIP family. Here we add to the growing evidence that NF-Y and bZIP form common complexes to affect many processes. We carried out transcriptional profiling on nf-yc mutants and through subsequent analyses found an enrichment of bZIP binding sites in the promoter elements of misregulated genes. Using NF-Y as bait, yeast two hybrid assays yielded interactions with bZIP proteins that are known to control ABA signaling. Accordingly, we find that plants mutant for several NF-Y subunits show characteristic phenotypes associated with the disruption of ABA signaling. While previous reports have shown additive roles for NF-YC family members in photoperiodic flowering, we found that they can have opposing roles in ABA signaling. Collectively, these results demonstrated the importance and complexity of NF-Y in the integration of environmental and hormone signals.

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Section: Resultssupporting

confidence: 93%

NUCLEAR FACTOR Y Transcription Factors Have Both Opposing and Additive Roles in ABA-Mediated Seed Germination

et al. 2013

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“…This suggests that low PAP levels during rosette development are critical to ensure proper rosette expansion in Arabidopsis. Whether this is linked to the reported influence of PAP on auxin (Robles et al., ; Zhang et al., ), ABA (Pornsiriwong et al., ; Rossel et al., ) or jasmonic acids (Rodríguez et al., ), or the interaction between SAL1/PAP and light perception (Chen & Xiong, ; Kim & von Arnim, ) will need to be further investigated, given that both phytohormones and phytochromes are known to regulate rosette growth and development.…”

Section: Discussionmentioning

confidence: 99%

Development of strategies for genetic manipulation and fine‐tuning of a chloroplast retrograde signal 3′‐phosphoadenosine 5′‐phosphate

et al. 2018

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Homeostasis of metabolism and regulation of stress-signaling pathways are important for plant growth. The metabolite 3 0 -phosphoadenosine-5 0 -phosphate (PAP)plays dual roles as a chloroplast retrograde signal during drought and high light stress, as well as a toxic by-product of secondary sulfur metabolism, and thus, its levels are regulated by the chloroplastic phosphatase, SAL1. Constitutive PAP accumulation in sal1 mutants improves drought tolerance but can impair growth and alter rosette morphology. Therefore, it is of interest to derive strategies to enable controlled and targeted PAP manipulation that could enhance drought tolerance while minimizing the negative effects on plant growth. We systematically tested the potential and efficiency of multiple established transgenic manipulation tools in altering PAP levels in Arabidopsis. Dexamethasone (dex)-inducible silencing of SAL1 via hpRNAi [pOpOff:SAL1hpRNAi] yielded reduction in SAL1 transcript and protein levels, yet failed to significantly induce PAP accumulation. Surprisingly, this was not due to insufficient silencing of the inducible system, as constitutive silencing using a strong promoter to drive hpRNAi and amiRNA targeting the SAL1 transcript also failed to increase PAP content or induce a sal1-like plant morphology despite significantly reducing the SAL1 transcript levels. In contrast, using dex-inducible expression of SAL1 cDNA to complement an Arabidopsis sal1 mutant successfully modulated PAP levels and restored rosette growth in a dosage-dependent manner.Results from this inducible complementation system indicate that plants with intermediate PAP levels could have improved rosette growth without compromising its drought tolerance. Additionally, preliminary evidence suggests that SAL1 cDNA driven by promoters of genes expressed specifically during early developmental stages such as ABA-Insensitive 3 (ABI3) could be another potential strategy for studying and optimizing PAP levels and drought tolerance while alleviating the negative impact of PAP on plant growth in sal1. Thus, we have identified ways that can allow future dissection into multiple aspects of stress and developmental regulation mediated by this chloroplast signal.--

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“…PAP accumulates when the SAL1 phosphatase that normally degrades this metabolite is inactivated during oxidative stress (Estavillo et al, 2011). SAL1-defective mutants show a short hypocotyl phenotype in the light, indicating that accumulation of PAP can repress hypocotyl elongation (Kim and von Arnim, 2009;Chen and Xiong, 2011). This phenotype is rescued (at least partially) in double sal1 phyB and sal1 hy5 mutants (Kim and von Arnim, 2009;Chen and Xiong, 2011), suggesting that functional phyB and HY5 are required for the PAP-promoted and lightdependent repression of hypocotyl growth.…”

Section: A Mechanistic Model For the Modulation Of Shade Elongation Rmentioning

confidence: 99%

“…SAL1-defective mutants show a short hypocotyl phenotype in the light, indicating that accumulation of PAP can repress hypocotyl elongation (Kim and von Arnim, 2009;Chen and Xiong, 2011). This phenotype is rescued (at least partially) in double sal1 phyB and sal1 hy5 mutants (Kim and von Arnim, 2009;Chen and Xiong, 2011), suggesting that functional phyB and HY5 are required for the PAP-promoted and lightdependent repression of hypocotyl growth. Further experiments should explore whether PAP is the retrograde signal deduced from our data to attenuate the response to shade in terms of hypocotyl elongation by independently inhibiting phyB deactivation, increasing HY5 accumulation, and promoting ABA signaling ( Figure 10).…”

Section: A Mechanistic Model For the Modulation Of Shade Elongation Rmentioning

confidence: 99%

Chloroplasts Modulate Elongation Responses to Canopy Shade by Retrograde Pathways Involving HY5 and Abscisic Acid

et al. 2019

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Plants use light as energy for photosynthesis but also as a signal of competing vegetation. Using different concentrations of norflurazon and lincomycin, we found that the response to canopy shade in Arabidopsis (Arabidopsis thaliana) was repressed even when inhibitors only caused a modest reduction in the level of photosynthetic pigments. High inhibitor concentrations resulted in albino seedlings that were unable to elongate when exposed to shade, in part due to attenuated light perception and signaling via phytochrome B and phytochrome-interacting factors. The response to shade was further repressed by a retrograde network with two separate nodes represented by the transcription factor LONG HYPOCOTYL 5 and the carotenoid-derived hormone abscisic acid. The unveiled connection among chloroplast status, light (shade) signaling, and developmental responses should contribute to achieve optimal photosynthetic performance under light-changing conditions.

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Genetic interaction of two abscisic acid signaling regulators, HY5 and FIERY1, in mediating lateral root formation

Cited by 14 publications

References 23 publications

NUCLEAR FACTOR Y Transcription Factors Have Both Opposing and Additive Roles in ABA-Mediated Seed Germination

NUCLEAR FACTOR Y Transcription Factors Have Both Opposing and Additive Roles in ABA-Mediated Seed Germination

Development of strategies for genetic manipulation and fine‐tuning of a chloroplast retrograde signal 3′‐phosphoadenosine 5′‐phosphate

Chloroplasts Modulate Elongation Responses to Canopy Shade by Retrograde Pathways Involving HY5 and Abscisic Acid

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