Genetic linkage map and expression analysis of genes expressed in the lamellae of the edible basidiomycete Pleurotus ostreatus

Peñas, María; Ramı́rez, Lucı́a; Pisabarro, Antonio G.

doi:10.1016/j.fgb.2006.01.008

Cited by 12 publications

(10 citation statements)

References 61 publications

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“…The availability of these strains, as well as a meiotic progeny derived from the N001 dikaryotic strain, enabled further genetic studies of P. ostreatus . Specifically, the molecular karyotype determined the presence of 11 chromosomes 24 that fit with 11 linkage groups described by our group in the genetic linkage map of P. ostreatus 25 , 26 . These studies supported the subsequent genome sequencing of PC9 and PC15 protoclones.…”

Section: Introductionsupporting

confidence: 71%

“…To rule out the possibility that such a finding was derived from an artificially skewed progeny, we revisited the P. ostreatus genetic linkage map. This map was constructed based on the segregation of 214 molecular markers in a population of 80 sibling monokaryons meiotically derived from N001, 26 and 68 of them were used in this study. According to the genetic map, 86% of the markers followed the expected 1:1 ratio.…”

Section: Resultsmentioning

confidence: 99%

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Somatic transposition and meiotically driven elimination of an active helitron family inPleurotus ostreatus

Borgognone¹,

Castanera²,

Muguerza³

et al. 2017

DNA Res

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Helitrons constitute a superfamily of DNA transposons that were discovered in silico and are widespread in most eukaryotic genomes. They are postulated to mobilize through a “rolling-circle” mechanism, but the experimental evidence of their transposition has been described only recently. Here, we present the inheritance patterns of HELPO1 and HELPO2 helitron families in meiotically derived progeny of the basidiomycete Pleurotus ostreatus. We found distorted segregation patterns of HELPO2 helitrons that led to a strong under-representation of these elements in the progeny. Further investigation of HELPO2 flanking sites showed that gene conversion may contribute to the elimination of such repetitive elements in meiosis, favouring the presence of HELPO2 vacant loci. In addition, the analysis of HELPO2 content in a reconstructed pedigree of subclones maintained under different culture conditions revealed an event of helitron somatic transposition. Additional analyses of genome and transcriptome data indicated that P. ostreatus carries active RNAi machinery that could be involved in the control of transposable element proliferation. Our results provide the first evidence of helitron mobilization in the fungal kingdom and highlight the interaction between genome defence mechanisms and invasive DNA.

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Section: Introductionsupporting

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Somatic transposition and meiotically driven elimination of an active helitron family inPleurotus ostreatus

Borgognone¹,

Castanera²,

Muguerza³

et al. 2017

DNA Res

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“…A similar situation was observed for LG7. In addition, a cluster of ligninolytic enzymes mapped to the upper end of LG6 (55).…”

Section: Resultsmentioning

confidence: 99%

Telomere Organization in the Ligninolytic Basidiomycete Pleurotus ostreatus

Pérez

Pangilinan

Pisabarro

et al. 2009

Appl Environ Microbiol

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Telomeres are structural and functional chromosome regions that are essential for the cell cycle to proceed normally. They are, however, difficult to map genetically and to identify in genome-wide sequence programs because of their structure and repetitive nature. We studied the telomeric and subtelomeric organization in the basidiomycete Pleurotus ostreatus using a combination of molecular and bioinformatics tools that permitted us to determine 19 out of the 22 telomeres expected in this fungus. The telomeric repeating unit in P. ostreatus is TTAGGG, and the numbers of repetitions of this unit range between 25 and 150. The mapping of the telomere restriction fragments to linkage groups 6 and 7 revealed polymorphisms compatible with those observed by pulsed field gel electrophoresis separation of the corresponding chromosomes. The subtelomeric regions in Pleurotus contain genes similar to those described in other eukaryotic systems. The presence of a cluster of laccase genes in chromosome 6 and a bipartite structure containing a Het-related protein and an alcohol dehydrogenase are especially relevant; this bipartite structure is characteristic of the Pezizomycotina fungi Neurospora crassa and Aspergillus terreus. As far as we know, this is the first report describing the presence of such structures in basidiomycetes and the location of a laccase gene cluster in the subtelomeric region, where, among others, species-specific genes allowing the organism to adapt rapidly to the environment usually map.Pleurotus ostreatus (Jacq.: Fr) Kumm. (Dikarya, Basidiomycota, Agaricomycotina, Agaricales) (52) is an active lignin degrader that lives as a saprophyte on dead or decaying wood. P. ostreatus (oyster mushroom) has been industrially cultivated for food production because of its flavor and its nutritional (49) and health-stimulating (8) properties. In addition, it produces various secondary metabolites of medical interest (33). P. ostreatus ligninolytic activity and enzymes have been used in the bioconversion of agricultural wastes (1); in the biodegradation of organopollutants, xenobiotics, and industrial contaminants (12); and in paper pulp bleaching (65), among other applications (10).The whole genome sequence of P. ostreatus is currently being assembled at the Joint Genome Institute (California). P. ostreatus is the first edible and the second lignin-degrading basidiomycete to be sequenced. The sequences of other basidiomycetes, such as Phanerochaete chrysosporium (48), Cryptococcus neoformans (44), Ustilago maydis (38), and Laccaria bicolor (47) have been published, and others (Postia placenta, Heterobasidion annosum, Agaricus bisporus, Serpula lacrymans, etc.) are in progress.Telomeres are the protective DNA-protein complexes found at chromosome termini (6, 13, 76). In most eukaryotes, telomeric DNA consists of tandem arrays of 5-to 8-bp direct repeats where specific telomere-capping proteins bind to ensure chromosomal-end integrity. Telomeres are essential for genome stability, and their shortening (attrition) can...

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“…Genetic maps of several other edible mushrooms, including Agaricus bisporus (Kerrigan et al 1993;Callac et al 1997;Moquet et al 1999;Foulongne-Oriol et al 2010, Pleurotus ostreatus (Larraya et al 2000;Park et al 2006;Sivolapova et al 2012), Pleurotus pulmonarius (Okuda et al 2009) and Pleurotus eryngii (Okuda et al 2012), have been reported previously. For L. edodes, several molecular genetic maps are currently available.…”

Section: Introductionmentioning

confidence: 88%

Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes

Gǒng

Liu

et al. 2014

Fungal Biology

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Genetic linkage map and expression analysis of genes expressed in the lamellae of the edible basidiomycete Pleurotus ostreatus

Cited by 12 publications

References 61 publications

Somatic transposition and meiotically driven elimination of an active helitron family inPleurotus ostreatus

Somatic transposition and meiotically driven elimination of an active helitron family inPleurotus ostreatus

Telomere Organization in the Ligninolytic Basidiomycete Pleurotus ostreatus

Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes

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