Genetic Mapping of Fall Armyworm Resistance in Zoysiagrass

Jessup, Russell W.; Renganayaki, K.; Reinert, James A.; Genovesi, A. D.; Engelke, M. C.; Paterson, Andrew H.; Kamps, Terry L.; Schulze, Stefan; Howard, Alma; Giliberto, B.; Burson, Byron L.

doi:10.2135/cropsci2010.09.0553

Cited by 13 publications

(13 citation statements)

References 54 publications

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“…Breeding objectives for zoysiagrasses are mainly to improve heat and drought tolerance, winter hardiness, disease and insect resistance. Several genetic maps of zoysiagrasses have been constructed to map targeted traits (Yaneshita et al ., ; Cai et al ., , ; Li et al ., , ; Jessup et al ., ). However, all current maps are partial, and the markers used in the previous studies, such as restriction fragment length polymorphism (RFLP) and amplified fragment length polymorphism (AFLP), are difficult to transfer between different mapping populations from one study to the next and are not suitable for integration of genetic maps with genome sequences.…”

Section: Introductionmentioning

confidence: 97%

Sequence‐tagged high‐density genetic maps of Zoysia japonica provide insights into genome evolution in Chloridoideae

et al. 2015

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Summary Zoysiagrass (Zoysia spp.), belonging to the genus Zoysia in the subfamily Chloridoideae, is widely used in domestic lawns, sports fields and as forage. We constructed high‐density genetic maps of Zoysia japonica using a restriction site‐associated DNA sequencing (RAD‐Seq) approach and an F1 mapping population derived from a cross between ‘Carrizo’ and ‘El Toro’. Two linkage maps were constructed, one for each of the parents. A map consisting of 2408 RAD markers distributed on 21 linkage groups was constructed for ‘Carrizo’. Another map with 1230 RAD markers mapped on 20 linkage groups was constructed for ‘El Toro’. The average distance between adjacent markers of the two maps was at 0.56 and 1.4 cM, respectively. Comparative genomics analysis was carried out among zoysiagrass, rice and sorghum genomes and a highly conserved collinearity in the gene order was observed among the three genomes. Chromosome collinearity was disrupted at centromeric regions for each chromosome pair between zoysiagrass and sorghum genomes. However, no obvious synteny gaps were observed across the centromeric regions between zoysiagrass and rice genomes. Two homologous chromosomes for each of the 10 sorghum chromosomes were found in the zoysiagrass genome, indicating an allotetraploid origin for zoysiagrass. The reduction of the basic chromosome number from 12 to 10 in chloridoids and panicoids took place via independent single‐step nested chromosome fusion events after the two subfamilies diverged from a common ancestor. The genetic maps will assist in genome sequence assembly, targeted gene isolation and comparative genomic analyses among grasses.

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Section: Introductionmentioning

confidence: 97%

Sequence‐tagged high‐density genetic maps of Zoysia japonica provide insights into genome evolution in Chloridoideae

et al. 2015

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“…Quantitative trait loci mapping for turfgrass has mainly been focused on disease and stress tolerance, and reproductive development related traits in ryegrass ( Lolium perenne L.), zoysiagrass ( Zoysia japonica Steud. ), and St. Augustinegrass [ Stenotaphrum secundatum (Walter) Kuntze; Forster et al, 2004; Ding et al, 2010; Jessup et al, 2011; Mulkey et al, 2014]. To date, no QTL mapping for establishment rate in bermudagrass has been reported.…”

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confidence: 99%

SSR Marker Development, Linkage Mapping, and QTL Analysis for Establishment Rate in Common Bermudagrass

Guo

Anderson

et al. 2017

The Plant Genome

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Common bermudagrass has been widely used as a major warmseason turf, forage, and soil stabilization grass in the southern United States. However, codominant marker development, linkage, and quantitative trait loci (QTL) mapping resources are limited in the important taxon. Accordingly, the objectives of this study were to develop simple sequence repeat (SSR) markers, construct a genetic map, and identify genomic regions associated with establishment rate. Five genomic SSR libraries were constructed, sequenced, and used in the development of 1003 validated SSR primer pairs (PPs). A linkage map was constructed using a first-generation selfed population derived from a genotype A12359 (2n = 4x = 36). A total of 249 polymorphic SSR PPs were mapped to 18 linkage groups (LGs). The total length of the map is 1094.7 cM, with an average marker interval of 4.3 cM. Ninety-eight out of 252 mapped loci (39%) were found to be distorted from the Mendelian 1:2:1 segregation ratio. Among the other 154 nondistorted loci, 88 coupling vs. 66 repulsion linkage phases were observed to confirm the allopolyploid origin of the parent. Ground coverage (GCR) phenotypic data in the establishment stage were collected in two replicated field trials. Quantitative trait loci mapping identified five genomic regions significantly related to the trait. The findings of this study provide valuable genetic tools and resources for genomic research, genetic improvement, and breeding new cultivars in the species.

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“…A high-density SSR genomic map has been completed (Li et al, 2009(Li et al, , 2010. Additionally, markers have been identified that are linked to a locus for fall armyworm (Spodoptera frugiperda) resistance (Jessup et al, 2011). Although 1044 SSRs have been developed for zoysiagrass (Cai et al, 2005), most are not publicly available (Jessup et al, 2011); however, a small number of zoysiagrass SSRs have been developed and released (Tsuruta et al, 2005).…”

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Detection of DNA and Ploidy Variation within Vegetatively Propagated Zoysiagrass Cultivars

Harris‐Shultz¹,

Milla‐Lewis²,

Patton³

et al. 2014

J. Amer. Soc. Hort. Sci.

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Zoysiagrass (Zoysia sp.) is used as a warm-season turfgrass for lawns, parks, and golf courses in the warm, humid and transitional climatic regions of the United States. Zoysiagrass is an allotetraploid species (2n = 4x = 40) and some cultivars are known to easily self- and cross-pollinate. Previous studies showed that genetic variability in the clonal cultivars Emerald and Diamond was likely the result of contamination (seed production or mechanical transfer) or mislabeling. To determine the extent of genetic variability of vegetatively propagated zoysiagrass cultivars, samples were collected from six commercially available zoysiagrass cultivars (Diamond, Emerald, Empire, JaMur, Meyer, Zeon) from five states (Arkansas, Florida, Georgia, North Carolina, Texas). Two of the newest cultivar releases (Geo and Atlantic) were to serve as outgroups. Where available, one sample from university research plots and two samples from sod farms were collected for each cultivar per state. Forty zoysiagrass simple sequence repeat (SSR) markers and flow cytometry were used to compare genetic and ploidy variation of each collected sample to a reference sample. Seventy-five samples were genotyped and an unweighted pair group method with arithmetic mean clustering revealed four groups. Group I (Z. japonica) included samples of ‘Meyer’ and Empire11 (‘Empire’ sample at location #11), Group II (Z. japonica × Z. pacifica) included samples of ‘Emerald’ and ‘Geo’, Group III (Z. matrella) included samples of ‘Diamond’ and ‘Zeon’, and Group IV (Z. japonica) consisted of samples from ‘Empire’, ‘JaMur’, ‘Atlantic’, and Meyer3 (‘Meyer’ at sample location #3). Samples of ‘Empire’, ‘Atlantic’, and ‘JaMur’ were indistinguishable with the markers used. Four samples were found to have alleles different from the respective reference cultivar, including two samples of ‘Meyer’, one sample of ‘Empire’, and one sample of ‘Emerald’. Three of these samples were from Texas and one of these samples was from Florida. Three of the four samples that were different from the reference cultivar were university samples. In addition, one sample, Empire11, was found to be an octoploid (2n = 8x = 80). For those samples that had a fingerprint different from the reference cultivar, contamination, selfing, and/or hybridization with other zoysiagrasses may have occurred.

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Genetic Mapping of Fall Armyworm Resistance in Zoysiagrass

Cited by 13 publications

References 54 publications

Sequence‐tagged high‐density genetic maps of Zoysia japonica provide insights into genome evolution in Chloridoideae

Sequence‐tagged high‐density genetic maps of Zoysia japonica provide insights into genome evolution in Chloridoideae

SSR Marker Development, Linkage Mapping, and QTL Analysis for Establishment Rate in Common Bermudagrass

Detection of DNA and Ploidy Variation within Vegetatively Propagated Zoysiagrass Cultivars

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