Genetic mapping of pollen fertility restoration QTLs in rye (Secale cereale L.) with CMS Pampa

Niedziela, Agnieszka; Brukwinski, W; Bednarek, Piotr Tomasz

doi:10.1007/s13353-020-00599-9

Cited by 6 publications

(11 citation statements)

References 59 publications

(120 reference statements)

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“…Preliminary, the S60/08 mapping population encompassed 130 lines, but due to the inbreeding depression, a phenomenon typical for allogamous species such as rye (Husband and Schemske 1996), 30 lines of mapping population was lost. As a result, map saturation was lower than in our previous study, whereas many as 175 RILs were used (Niedziela et al 2021). Still, all linkage groups' marker saturation was sufficient for quantitative analysis, possibly due to many recombinations typical for such populations (Xu et al 2017).…”

Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

“…We have successfully applied SNP and silicoDArT markers for genotyping RIL-based mapping populations of rye (Niedziela et al 2021 ) and triticale (Wasiak et al 2021 ). The NGS markers proved to efficient in identifying many markers that were more or less evenly distributed along species chromosomes saturated maps with 1.66 (rye) and 1.70 (triticale) markers per every one cM (Niedziela et al 2021 ; Wasiak et al 2021 ). Preliminary, the S60/08 mapping population encompassed 130 lines, but due to the inbreeding depression, a phenomenon typical for allogamous species such as rye (Husband and Schemske 1996 ), 30 lines of mapping population was lost.…”

Section: Discussionmentioning

confidence: 99%

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New PCR-specific markers for pollen fertility restoration QRfp-4R in rye (Secale cereale L.) with Pampa sterilizing cytoplasm

2021

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Pampa cytoplasmic male sterility phenomenon is used extensively in the rye hybrid breeding programs. It relies on sterilizing action of the cytoplasm resulting in non-viable pollen of female lines. The sterilizing effect is problematic for reversion, and efficient restores are needed. The most promising QTL is located on chromosome 4R, but other chromosomes may also code the trait. Advanced recombinant inbred lines formed bi-parental mapping population genotyped with DArTseq markers. Genetic mapping allowed the seven linkage groups to construct with numerous markers and represent all rye chromosomes. Single marker analysis and composite interval mapping were conducted to identify markers linked to the pollen fertility. Association mapping was used to detect additional markers associated with the trait. A highly significant QTL (QRfp-4R) that explained 42.3% of the phenotypic variation was mapped to the distal part of the long arm of the 4R chromosome. The markers localized in the QRfp-4R region achieve R2 association values up to 0.59. The homology of the 43 marker sequences to the loci responsible for fertility restoration in other species and transcription termination factor (mTERF) linked to Rf genes was established. Ten markers were successfully converted into PCR-specific conditions, and their segregation pattern was identical to that of unconverted DArTs.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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New PCR-specific markers for pollen fertility restoration QRfp-4R in rye (Secale cereale L.) with Pampa sterilizing cytoplasm

2021

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“…In hybrid rye, numerous CMS systems exist, of which the most predominant is the Pampa (P) type [6]. In this system, five major P-type Rf genes have been identified on 1RS, 4RL (Rfp1, Rfp2, Rfp3), and 6R (dominant modifier) chromosomes, and three minor genes on 3RL, 4RL, and 5R chromosomes [7][8][9][10]. The less prevalent CMS systems include the 'Gülzow' (G) type Int.…”

Section: Introductionmentioning

confidence: 99%

Genomic Scan of Male Fertility Restoration Genes in a ‘Gülzow’ Type Hybrid Breeding System of Rye (Secale cereale L.)

Vendelbo

Mahmood²,

Sarup³

et al. 2021

IJMS

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Efficient and stable restoration of male fertility (Rf) is a prerequisite for large-scale hybrid seed production but remains an inherent issue in the predominant fertility control system of rye (Secale cereale L.). The ‘Gülzow’ (G)-type cytoplasmic male sterility (CMS) system in hybrid rye breeding exhibits a superior Rf. While having received little scientific attention, one major G-type Rf gene has been identified on 4RL (Rfg1) and two minor genes on 3R (Rfg2) and 6R (Rfg3) chromosomes. Here, we report a comprehensive investigation of the genetics underlying restoration of male fertility in a large G-type CMS breeding system using recent advents in rye genomic resources. This includes: (I) genome-wide association studies (GWAS) on G-type germplasm; (II) GWAS on a biparental mapping population; and (III) an RNA sequence study to investigate the expression of genes residing in Rf-associated regions in G-type rye hybrids. Our findings provide compelling evidence of a novel major G-type non-PPR Rf gene on the 3RL chromosome belonging to the mitochondrial transcription termination factor gene family. We provisionally denote the identified novel Rf gene on 3RL RfNOS1. The discovery made in this study is distinct from known P- and C-type systems in rye as well as recognized CMS systems in barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.). We believe this study constitutes a stepping stone towards understanding the restoration of male fertility in the G-type CMS system and potential resources for addressing the inherent issues of the P-type system.

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“…In this system five major P-type Rf genes have been identified on 1RS, 4RL (Rfp1, Rfp2, Rfp3) and 6R (dominant modifier) chromosome, and three minor genes on 3RL, 4RL and 5R chromosome in 'Pampa' (P) type cytoplasm [7][8][9][10] . Less prevalent CMS systems include 'Gülzow' (G) type originating from the Austrian population of rye variety 'Schlägler alt' 11 , R-type originating from a Russian population, 12 , C- 13 and S- 14 type originating from an old Polish cultivar 'Smolickie'.…”

Section: Graphical Abstract Introductionmentioning

confidence: 99%

Genetic architecture of male fertility restoration in a hybrid breeding system of rye (Secale cereale L.)

Vendelbo¹,

Mahmood²,

Sarup³

et al. 2021

Preprint

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The ‘Gülzow’ (G) type cytoplasmic male sterility (CMS) system in hybrid rye (Secale cereale L.) breeding exhibits a strong and environmentally stable restoration of male fertility (Rf). While having received little scientific attention, three G-type Rf genes had been identified on 4RL (Rfg1) and two minor genes on 3R (Rfg2) and 6R (Rfg3) chromosome. Here, we report a comprehensive investigation of the genetics underlying restoration of male fertility in a large G-type CMS breeding system using a palette of complementing forward and reverse genetic analysis. This includes (i) genome wide association studies (GWAS) on a G-type germplasm, (ii) GWAS on a biparental mapping population, (iii) in silico identification of Rf-like pentatricopeptide repeat (RFL-PPR) genes and their expressed in G-type rye hybrids, and (iv) mining patterns in linkage disequilibrium. Our findings provide compelling evidence of a novel major G-type non-PPR Rf gene on the 3RL chromosome. In the in silico analysis, we identified 22 RFL-PPR of which 15 were expressed in the transcriptome of G-type hybrids. Our findings provides a novel insight into the underlying genetics of male fertility restoration in a G-type CMS system in rye. The discovery made in this study is distinct to known P- and C-type systems in rye in addition to known CMS systems in barley and wheat. This study constitutes a steppingstone towards understanding the restoration of male fertility in G-type CMS system and a potential resources for addressing the inherent issues of the P-type system.

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Genetic mapping of pollen fertility restoration QTLs in rye (Secale cereale L.) with CMS Pampa

Cited by 6 publications

References 59 publications

New PCR-specific markers for pollen fertility restoration QRfp-4R in rye (Secale cereale L.) with Pampa sterilizing cytoplasm

New PCR-specific markers for pollen fertility restoration QRfp-4R in rye (Secale cereale L.) with Pampa sterilizing cytoplasm

Genomic Scan of Male Fertility Restoration Genes in a ‘Gülzow’ Type Hybrid Breeding System of Rye (Secale cereale L.)

Genetic architecture of male fertility restoration in a hybrid breeding system of rye (Secale cereale L.)

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