Genetic Marker Mediated Transfer of an Alien Gene,<i>PM21</i>, into Wheat Conferring Resistance to Powdery Mildew

Yıldırım, Ahmet; Sakin, Mehmet Ali; Karadağ, Yaşar; Gökmen, Sabri; Kandemır, N.; Akkaya, Mahinur S.; Yildirim, F.

doi:10.1080/13102818.2004.10817081

Cited by 6 publications

(2 citation statements)

References 16 publications

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“…In this study, a total of 3 molecular markers were used to detect Pm21 in breeding material, SCAR 1250 , SCAR 1400 , and co-dominant PCR marker NAU/xibao, all of which appeared to be suitable for Liu et al (1999), Yildirim et al (2004), and Cao et al (2006). Other markers were previously used to detect Pm21 and identify homozygous and heterozygous translocation in breeding materials, these include specific RAPD markers, such as OPH17-1900 and OPH17-1000 (Qi et al 1996).…”

Section: Resultsmentioning

confidence: 99%

“…In foreground selection to detect the Pm21 gene in plant material, three molecular markers were used: SCAR 1250 (1250 bp), SCAR 1400 (1400 bp) (Liu et al 1999;Yildirim et al 2004), and NAU/xibao (902 bp) (Cao et al 2006). The 10-µl reaction mixture contained the following components: 4 µl of DNA solution, 1 × buffer (MBI Fermentas), 2.5mM MgCl 2 , 0.2mM dNTPs, 0.5µM primer, and 1 U of Taq DNA polymerase (MBI Fermentas).…”

Section: Foreground Selection (Fs)mentioning

confidence: 99%

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Lr39 + Pm21: a new effective combination of resistance genes for leaf rust and powdery mildew in wheat

Pietrusińska¹,

Czembor²,

Czembor³

2013

CZECH J GENET PLANT

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Pietrusińska A., Czembor P.Cz., Czembor J.H. (2013): Lr39 + Pm21: a new effective combination of resistance genes for leaf rust and powdery mildew in wheat. Czech J. Genet. Plant Breed., 49: 109-115.Two effective resistance genes were introduced, one for leaf rust (Lr39) and the other for powdery mildew (Pm21), into the susceptible German wheat cv. Lexus. Molecular selection of plant material was carried out using closely linked markers to the introduced genes (foreground selection). In addition, for the BC 1 F 1 population, background selection was carried out using AFLP markers that were distributed randomly throughout the wheat genome. Moreover, resistance tests were conducted using natural pathogen populations of Puccinia triticina and Blumeria graminis. The use of molecular markers for foreground selection in combination with pathology tests enabled 66 homozygous lines to be obtained that were simultaneously resistant to leaf rust and powdery mildew.

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Section: Resultsmentioning

confidence: 99%

Section: Foreground Selection (Fs)mentioning

confidence: 99%

Lr39 + Pm21: a new effective combination of resistance genes for leaf rust and powdery mildew in wheat

Pietrusińska¹,

Czembor²,

Czembor³

2013

CZECH J GENET PLANT

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Suppressed recombination rate in 6VS/6AL translocation region carrying the Pm21 locus introgressed from Haynaldia villosa into hexaploid wheat

et al. 2011

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Identification of a New Powdery Mildew Resistance Gene pmDHT at or Closely Linked to the Pm5 Locus in the Chinese Wheat Landrace Dahongtou

Qie

Yuan

et al. 2019

Plant Disease

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Chinese wheat landrace Dahongtou was resistant to 35 of 38 tested Chinese isolates of Blumeria graminis f. sp. tritici at the seedling stage. Genetic analysis of the F2 populations and their derived F2:3 families of crosses of Dahongtou with the susceptible varieties Mingxian 169 and Huixianhong indicated that the resistance of Dahongtou to B. graminis f. sp. tritici isolate E09 was conferred by a single recessive gene, tentatively designated as pmDHT. The gene was mapped to chromosome arm 7BL and flanked by markers Xwmc526/XBE443877 and Xgwm611/Xwmc511 at genetic distances of 0.8 and 0.3 cM, respectively. The chromosomal position of pmDHT was similar to the multi-allelic Pm5 locus on 7BL. Allelism tests with crosses of Dahongtou with Fuzhuang 30 (Pm5e) and Xiaobaidong (mlxbd) indicated that pmDHT was allelic to both Pm5e and mlxbd. However, pmDHT showed a different pattern of resistance to the 38 B. graminis f. sp. tritici isolates compared with wheat lines with Pm5a, Pm5b, Pm5e, mlxbd, and PmHYM and also differed from PmSGA. Thus, pmDHT was identified most likely as a new allele or at least a closely linked gene of the Pm5 locus. This gene can be transferred into susceptible wheat cultivars/lines and pyramided with other resistance genes through marker-assisted selection to improve powdery mildew resistance.

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Genetic Marker Mediated Transfer of an Alien Gene,PM21, into Wheat Conferring Resistance to Powdery Mildew

Cited by 6 publications

References 16 publications

Lr39 + Pm21: a new effective combination of resistance genes for leaf rust and powdery mildew in wheat

Lr39 + Pm21: a new effective combination of resistance genes for leaf rust and powdery mildew in wheat

Suppressed recombination rate in 6VS/6AL translocation region carrying the Pm21 locus introgressed from Haynaldia villosa into hexaploid wheat

Identification of a New Powdery Mildew Resistance Gene pmDHT at or Closely Linked to the Pm5 Locus in the Chinese Wheat Landrace Dahongtou

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