2004
DOI: 10.1111/j.1462-2920.2004.00596.x
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Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways

Abstract: Ralstonia eutropha JMP134 (pJP4) is a useful model for the study of bacterial degradation of substituted aromatic pollutants. Several key degrading capabilities, encoded by tfd genes, are located in the 88 kb, self-transmissible, IncP-1 beta plasmid pJP4. The complete sequence of the 87,688 nucleotides of pJP4, encoding 83 open reading frames (ORFs), is reported. Most of the coding sequence corresponds to a well-conserved IncP-1 beta backbone and the previously reported tfd genes. In addition, we found hypothe… Show more

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Cited by 136 publications
(145 citation statements)
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“…Forty-three ORFs correspond to a well-conserved IncP-1b backbone, and one corresponds to parA, encoding a resolvase, ParA. This partitioning gene parA is present as an intact gene, as is the case for the IncP1b plasmids pB2, pB3, pJP4 and pA1 (Heuer et al, 2004;Trefault et al, 2004;Harada et al, 2006). Plasmid pBP136 possesses a classical IncP-1b backbone: two regions involved in plasmid conjugation (the tra and trb operons), and a region carrying the genes for plasmid replication, central control, stable inheritance and partitioning (trfA, klc, kor, kle, inc, kfrA and parA).…”
Section: Nucleotide Sequence and Orfs Of Pbp136mentioning
confidence: 99%
“…Forty-three ORFs correspond to a well-conserved IncP-1b backbone, and one corresponds to parA, encoding a resolvase, ParA. This partitioning gene parA is present as an intact gene, as is the case for the IncP1b plasmids pB2, pB3, pJP4 and pA1 (Heuer et al, 2004;Trefault et al, 2004;Harada et al, 2006). Plasmid pBP136 possesses a classical IncP-1b backbone: two regions involved in plasmid conjugation (the tra and trb operons), and a region carrying the genes for plasmid replication, central control, stable inheritance and partitioning (trfA, klc, kor, kle, inc, kfrA and parA).…”
Section: Nucleotide Sequence and Orfs Of Pbp136mentioning
confidence: 99%
“…EbN1, BenK from Rhodococcus jostii RHA1 and a putative A. baylyi ADP-1 transport gene. For ABC-type transporters, the sequences from Azoarcus evansii were selected, along with a putative ABC transporter of unknown function from plasmid pJP4 (Trefault et al, 2004). As an additional type of transporter gene, the TRAP family transporter (Chae & Zylstra, 2006) was also included in the search.…”
Section: Resultsmentioning
confidence: 99%
“…The cellular machinery encoded by the microbial mer operon on pJP4 provides specific uptake proteins (MerP and MerT) which transport Hg 2+ in the cytoplasm of the host cell and which prevent damage to the cell. In the cell, Hg 2+ is reduced with NADPH to Hg 0 by the enzyme mercuric reductase (MerA), which is related to glutathione reductase 8) . The rate-limiting step for the overall detoxification reaction is probably uptake into the host cell, rather than reduction of Hg 2+ in the cell 19) .…”
Section: Discussionmentioning
confidence: 99%
“…This 88-kb broad-host-range plasmid of incompatibility group P-1β harbors genes related to mercury resistance and key catabolic functions for 2.4-dichlorophenoxyacetic acid (2,4-D) 7,8) . The E. coli K-12 strain HB101 is a restrictionnegative streptomycin (Sm)-resistant strain that requires thiamine, leucine, and proline for growth 15) .…”
Section: Bacterial Strainsmentioning
confidence: 99%
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