Genetic Origin of Malignant Trophoblastic Neoplasms Analyzed by Sequence Tag Site Polymorphic Markers

Shahib, Nurhalim; Martaadisoebrata, Djamhoer; Kondo, Haruhiko; Zhou, Yong; Shinkai, N; Nishimura, Chisa; Kato, Kiyoko; Matsuda, Takao; Wake, Norio; Kato, Hidenori

doi:10.1006/gyno.2001.6145

Cited by 18 publications

(9 citation statements)

References 16 publications

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“…Other women in our study had a history of HM, with or without GTN, and had miscarriages or term pregnancies afterwards, leading to difficulties in determining the causative pregnancy. Previously, other authors demonstrated using molecular genetic techniques that the immediate antecedent pregnancy may not be the causative conception in some cases of choriocarcinoma 29,30 . Genetic techniques are also used to differentiate gestational from non‐gestational trophoblastic neoplasia.…”

Section: Discussionmentioning

confidence: 99%

Fatal cases of gestational trophoblastic neoplasia over four decades in the Netherlands: a retrospective cohort study

Lybol¹,

Centen²,

Thomas³

et al. 2012

BJOG

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Objective To describe fatal cases of gestational trophoblastic neoplasia (GTN) over four decades and evaluate whether treatment was given according to the protocol and reveal possible implications for future management.Design Retrospective cohort study.Setting The Netherlands.Population Women who died from GTN from 1971 to 2011.Methods Records from the Dutch Central Registry for Hydatidiform Moles and the Working Party on Trophoblastic Disease were used to identify fatal cases of GTN.Main outcome measures Disease extent, risk classification, treatment regimens and cause of death.Results Twenty-six women died from GTN. In five cases GTN developed after a hydatidiform mole and in 19 cases following term pregnancy. Half of the women died between 1971 and 1980, when women were not yet classified as having low-risk or highrisk disease and were therefore not yet treated accordingly. A major decline in the number of deaths was seen after the first decade, with a further decrease from 1981 to 2011. Early death occurred in nine women. In four of these women, death was treatment-related. Women who died more than 4 weeks after the start of treatment mostly died from metastatic tumour (n = 14).Conclusions The yearly number of women who died from GTN decreased considerably over the last four decades. Appropriate risk classification is essential to start optimal initial therapy and to prevent therapy resistance. Women with post-term choriocarcinoma represented a large proportion of the dead women and we propose that these women are considered as having high-risk disease.

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Section: Discussionmentioning

confidence: 99%

Fatal cases of gestational trophoblastic neoplasia over four decades in the Netherlands: a retrospective cohort study

Lybol¹,

Centen²,

Thomas³

et al. 2012

BJOG

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“…Besides determination of purity by OD260/280 measurement, RNA integrity was checked by gel electrophoresis in formaldehyde gels. RNA from androgenetic placental tissues (n ¼ 4) (gestational weeks 8 -12) was obtained from two complete hydatidiform moles of dispermic origin and two complete hydatidiform moles of monospermic origin as confirmed by microsatellite analysis (Shahib et al, 2001). Tissue fragments of molar pregnancy samples were transported in RNA stabilization solution (RNALater; Ambion) and subsequently processed identically as done for normal placental tissues.…”

Section: Expression Analysis In Normal and Androgenetic Placental Tismentioning

confidence: 99%

The parent-of-origin effect of 10q22 in pre-eclamptic females coincides with two regions clustered for genes with down-regulated expression in androgenetic placentas

Oudejans¹,

Mulders²,

Lachmeijer³

et al. 2004

MHR: Basic Science of Reproductive Medicine

101

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By affected sib-pair linkage analysis of 24 families with pre-eclampsia, we confirm a susceptibility locus on chromosome 10q22.1 in Dutch females: a multipoint non-parametric linkage score of 3.6 near marker D10S1432 was obtained. Haplotype analysis showed a parent-of-origin effect: maximal allele sharing in the affected sibs was found for maternally derived alleles in all families, but not for the paternally derived alleles. As matrilineal inheritance suggests the presence of maternally expressed imprinted genes, while imprinting operates predominantly in (extra)embryonic tissues, all genes (n=132) known on 10q22 between GATA121A08 and D10S580 were screened for seven sequence-related features associated with imprinting and subsequently tested for expression in first trimester placenta. Placental expression of genes selected in this way (n=55) was compared with expression in androgenetic placentas of identical gestational age. Two regions on 10q22 were identified with developmentally co-repressed genes with non-random chromosomal distribution. Interestingly, these two clusters, near CTNNA3 and KCNMA1 and each containing five genes with down-regulated expression in androgenetic placentas, coincided with the regions with maximal maternal allele sharing seen in the pre-eclamptic sisters. Our linkage and expression data are compatible with the concept that pre-eclampsia involves maternally expressed imprinted genes that operate in the first trimester placenta.

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“…Accordingly, chemotherapy for this patient was limited to only one course with MAC, thereafter hCG levels fell rapidly to extremely low titer. DNA studies have confirmed the gestational origin of PSTTs and have shown that PSTTs can arise from normal conception 4,5 …”

Section: Discussionmentioning

confidence: 93%

“…DNAs in the peripheral mononuclear cells from parents’ blood were obtained using TLE solution (GenTLE/TM, Takara, Osaka, Japan) and 2‐propanol precipitation. These DNAs were subjected to PCR (annealing temperature 55°C with 32 cycles) with sequence tagged site (STS) polymorphic primers 3–5 …”

Section: Case Reportmentioning

confidence: 99%

Placental site trophoblastic tumor of the uterine cervix occurring from undetermined antecedent pregnancy

Soma

Okada

Yoshinari

et al. 2004

J of Obstet and Gynaecol

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A cervical polyp complicated by severe hemorrhage was removed from a 43-year-old Japanese woman (gravida 0), who had undergone tubectomy on the right side 10 years previously. The polyp was diagnosed by immunohistochemical studies as placental site trophoblastic tumor of the cervix, but no metastatic foci were found in any other uterine site. The tumor was further demonstrated by PCR polymorphisms to possess two genomic DNA of the patient and her husband. Serum beta-hCG and urinary hCG titers were both low, which rapidly fell to 0.8 mIU/mL after a total hysterectomy and remained 0.2 mIU/mL after dismission. She has been uneventful for 3 years.

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Genetic Origin of Malignant Trophoblastic Neoplasms Analyzed by Sequence Tag Site Polymorphic Markers

Cited by 18 publications

References 16 publications

Fatal cases of gestational trophoblastic neoplasia over four decades in the Netherlands: a retrospective cohort study

Fatal cases of gestational trophoblastic neoplasia over four decades in the Netherlands: a retrospective cohort study

The parent-of-origin effect of 10q22 in pre-eclamptic females coincides with two regions clustered for genes with down-regulated expression in androgenetic placentas

Placental site trophoblastic tumor of the uterine cervix occurring from undetermined antecedent pregnancy

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