Genetic polymorphism at K-Casein gene in indian camel breeds (Camelus dromdarius)

Jadhav, S.A.; Umrikar, U. D.; Sawane, M. P.; Pawar, Vikrant; Deshmukh, Rahul S.; Dahiya, Shyam Singh; Mehta, S. L.

doi:10.5958/2277-8934.2020.00029.6

Cited by 3 publications

(1 citation statement)

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“…Previous studies on milk traits in camels have mainly focused on physiological changes and biochemical indicators in milk and blood; few studies have investigated gene expression in relation to milk traits. To date, only a few studies have reported the association between Casein gene (CSN2 and CSN3) polymorphisms and milk composition traits in camels [13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of the Bactrian camel (Camelus bactrianus) reveals candidate genes affecting milk production traits

Yao,

Dou,

Zhao

et al. 2023

BMC Genomics

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Background Milk production traits are complex traits with vital economic importance in the camel industry. However, the genetic mechanisms regulating milk production traits in camels remain poorly understood. Therefore, we aimed to identify candidate genes and metabolic pathways that affect milk production traits in Bactrian camels. Methods We classified camels (fourth parity) as low- or high-yield, examined pregnant camels using B-mode ultrasonography, observed the microscopic changes in the mammary gland using hematoxylin and eosin (HE) staining, and used RNA sequencing to identify differentially expressed genes (DEGs) and pathways. Results The average standard milk yield over the 300 days during parity was recorded as 470.18 ± 9.75 and 978.34 ± 3.80 kg in low- and high-performance camels, respectively. Nine female Junggar Bactrian camels were subjected to transcriptome sequencing, and 609 and 393 DEGs were identified in the low-yield vs. high-yield (WDL vs. WGH) and pregnancy versus colostrum period (RSQ vs. CRQ) comparison groups, respectively. The DEGs were compared with genes associated with milk production traits in the Animal Quantitative Trait Loci database and in Alashan Bactrian camels, and 65 and 46 overlapping candidate genes were obtained, respectively. Functional enrichment and protein–protein interaction network analyses of the DEGs and candidate genes were conducted. After comparing our results with those of other livestock studies, we identified 16 signaling pathways and 27 core candidate genes associated with maternal parturition, estrogen regulation, initiation of lactation, and milk production traits. The pathways suggest that emerged milk production involves the regulation of multiple complex metabolic and cellular developmental processes in camels. Finally, the RNA sequencing results were validated using quantitative real-time PCR; the 15 selected genes exhibited consistent expression changes. Conclusions This study identified DEGs and metabolic pathways affecting maternal parturition and milk production traits. The results provides a theoretical foundation for further research on the molecular mechanism of genes related to milk production traits in camels. Furthermore, these findings will help improve breeding strategies to achieve the desired milk yield in camels. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of the Bactrian camel (Camelus bactrianus) reveals candidate genes affecting milk production traits

Yao,

Dou,

Zhao

et al. 2023

BMC Genomics

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Genetic divergence of two casein genes and correlated milk traits in Maghrebi camels

Darwish

Abdelhafez

El-Metwaly³

et al. 2022

Biologia

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Comparative Analysis of the Polymorphism of the Casein Genes in Camels Bred in Kazakhstan

Amandykova

Dossybayev

Mussayeva³

et al. 2022

Diversity

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Caseins play an important role in determining the technological properties and quantitative characteristics of camel milk. To date, only a few studies on the genetic polymorphism of casein genes have been reported in the camel populations of Kazakhstan. Therefore, this work aimed to identify the genetic polymorphism level of casein genes among camel populations of the Almaty region of Kazakhstan. The PCR-RFLP method was used for this purpose and the following genotypes were revealed as a result: CSN3 gene—CC, CT, TT, where the T allele predominated in all populations, with a frequency of 0.60; CSN2 gene—AA, AG, GG, with the predomination of A allele (0.64); and CSN1S1 gene—GG and GT, with the predomination of G allele (0.94). Statistical analysis was carried out using the POPGENE and GenAlEx software. The χ2 values were equal to 12.1 (CSN3), 8.6 (CSN2), and 14.5 (CSN1S1). As a result, three out of 53 animals were designated as the “core” of the population—animals with the desired genotypes: CC genotype for the CSN3 gene and AA genotype for the CSN2 gene. Such animals can be selected for further use with an increase in the number of livestock with high productivity rates.

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Genetic polymorphism at K-Casein gene in indian camel breeds (Camelus dromdarius)

Cited by 3 publications

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Transcriptome analysis of the Bactrian camel (Camelus bactrianus) reveals candidate genes affecting milk production traits

Transcriptome analysis of the Bactrian camel (Camelus bactrianus) reveals candidate genes affecting milk production traits

Genetic divergence of two casein genes and correlated milk traits in Maghrebi camels

Comparative Analysis of the Polymorphism of the Casein Genes in Camels Bred in Kazakhstan

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