Genetic polymorphism of Merozoite Surface Protein-2 (MSP-2) in Plasmodium falciparum isolates from Pawe District, North West Ethiopia

Mohammed, Hussein; Kassa, Moges; Assefa, Ashenafi; Tadesse, Mekonnen; Kebede, Amha

doi:10.1371/journal.pone.0177559

Cited by 27 publications

(36 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study found that nearly three-quarters of the isolates harboured multiple genotypes, with about one-quarter monoclonal. This is a similar finding to a previous study from Northwest Ethiopia that only analysed msp - 2 [ 16 ]. However, it is a significantly higher proportion with multiple clones than many of the locations sampled by the International Centers of Excellence for Malaria Research [ 9 ].…”

Section: Discussionsupporting

confidence: 92%

“…To date, there has been limited assessment of genetic diversity of P. falciparum in Ethiopia [ 15 , 16 ], despite scaling up of national malaria control interventions [ 17 ]. This study aimed to characterize the genetic diversity and allele frequencies of msp - 1, msp - 2 and glurp genes of P. falciparum isolates from uncomplicated malaria patients in Humera along the Ethiopian border.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity of the msp-1, msp-2, and glurp genes of Plasmodium falciparum isolates in Northwest Ethiopia

Mohammed

Kassa

Mekete

et al. 2018

Malar J

Self Cite

View full text Add to dashboard Cite

BackgroundDetermination of the genetic diversity of malaria parasites can inform the intensity of transmission and identify potential deficiencies in malaria control programmes. This study was conducted to characterize the genetic diversity and allele frequencies of Plasmodium falciparum in Northwest Ethiopia along the Eritrea and Sudan border.MethodsA total of 90 isolates from patients presenting to the local health centre with uncomplicated P. falciparum were collected from October 2014 to January 2015. DNA was extracted and the polymorphic regions of the msp-1, msp-2 and glurp loci were genotyped by nested polymerase chain reactions followed by gel electrophoresis for fragment analysis.ResultsAllelic variation in msp-1, msp-2 and glurp were identified in 90 blood samples. A total of 34 msp alleles (12 for msp-1 and 22 for msp-2) were detected. For msp-1 97.8% (88/90), msp-2 82.2% (74/90) and glurp 46.7% (42/90) were detected. In msp-1, MAD20 was the predominant allelic family detected in 47.7% (42/88) of the isolates followed by RO33 and K1. For msp-2, the frequency of FC27 and IC/3D7 were 77% (57/74) and 76% (56/74), respectively. Nine glurp RII region genotypes were identified. Seventy percent of isolates had multiple genotypes and the overall mean multiplicity of infection was 2.6 (95% CI 2.25–2.97). The heterozygosity index was 0.82, 0.62 and 0.20 for msp-1, msp-2 and glurp, respectively. There was no significant association between multiplicity of infection and age or parasite density.ConclusionsThere was a high degree of genetic diversity with multiple clones in P. falciparum isolates from Northwest Ethiopia suggesting that there is a need for improved malaria control efforts in this region.Electronic supplementary materialThe online version of this article (10.1186/s12936-018-2540-x) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the msp-1, msp-2, and glurp genes of Plasmodium falciparum isolates in Northwest Ethiopia

Mohammed

Kassa

Mekete

et al. 2018

Malar J

Self Cite

View full text Add to dashboard Cite

show abstract

“…3 ), most likely because the frequency of infectious mosquito bites in high transmission settings, that could result in additional inoculum of variant parasites is higher than in lower transmission settings [ 49 ]. This finding supports general notion that Low transmission settings are commonly associated with reduced MOI in malaria endemic countries [ 50 , 51 ]. The geometric mean MOI differed significantly across the time points in Abura (Fig.…”

Section: Discussionsupporting

confidence: 90%

Seasonal variations in Plasmodium falciparum genetic diversity and multiplicity of infection in asymptomatic children living in southern Ghana

et al. 2018

View full text Add to dashboard Cite

BackgroundGenetic diversity in Plasmodium falciparum (P. falciparum) parasites is a major hurdle to the control of malaria. This study monitored changes in the genetic diversity and the multiplicity of P. falciparum parasite infection in asymptomatic children living in southern Ghana at 3 month intervals between April 2015 and January 2016.MethodsFilter paper blood spots (DBS) were collected quarterly from children living in Obom, a community with perennial malaria transmission and Abura, a community with seasonal malaria transmission. Genomic DNA was extracted from the DBS and used in polymerase chain reaction (PCR)-based genotyping of the merozoite surface protein 1 (msp 1) and merozoite surface protein 2 (msp 2) genes.ResultsOut of a total of 787 samples that were collected from the two study sites, 59.2% (466/787) tested positive for P. falciparum. The msp 1 and msp 2 genes were successfully amplified from 73.8% (344/466) and 82.5% (385/466) of the P. falciparum positive samples respectively. The geometric mean MOI in Abura ranged between 1.17 (95% CI: 1.08–1.28) and 1.48 (95% CI: 1.36–1.60) and was significantly lower (p < 0.01, Dunn’s multiple comparison test) than that determined in Obom, where the geometric mean MOI ranged between 1.82 (95% CI: 1.58–2.08) and 2.50 (95% CI: 2.33–2.678) over the study period. Whilst the msp 1 R033:MAD20:KI allelic family ratio was dynamic, the msp 2 3D7:FC27 allelic family ratio remained relatively stable across the changing seasons in both sites.ConclusionsThis study shows that seasonal variations in parasite diversity in these communities can be better estimated by msp 1 rather than msp 2 due to the constantly changing relative intra allelic frequencies observed in msp 1 and the fact that the dominance of any msp 2 allele was dependent on the transmission setting but not on the season as opposed to the dominance of any msp 1 allele, which was dependent on both the season and the transmission setting.Electronic supplementary materialThe online version of this article (10.1186/s12879-018-3350-z) contains supplementary material, which is available to authorized users.

show abstract

“…In this study, 80% of the isolates harboured more than one parasite genotype identi ed by the presence of two or more alleles of one or both genes with the overall mean MOI being 3.2 (95% CI: 2.87-3.46). The overall MOI value reported in this study was higher than previously reported studies, including Ethiopia (MOI: 1.8 -2.6) between 2015-2018 [42,43,44], Brazzaville, Republic of Congo (MOI: 2.2) [16] in 2011 and Bobo-Dioulasso, Burkina Faso (MOI: 1.95) [62]. In contrast to study reported in Bioko Island, Equatorial Guinea (MOI: 5.51) [53] in 2018 and Gabon (MOI: 4.0) [63] in 2018.…”

Section: Discussioncontrasting

confidence: 83%

Genetic diversity and genotype multiplicity of Plasmodium falciparum infection in patients with uncomplicated malaria in Chewaka district, Ethiopia

Abamecha

El-Abid

Yilma

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Genetic diversity in Plasmodium falciparum poses a major threat to malaria control and elimination interventions. Characterization of the genetic diversity of P. falciparum strains can be used to assess intensity of parasite transmission and identify potential deficiencies in malaria control programmes, which provides vital information to evaluating malaria elimination efforts. This study investigated the P. falciparum genetic diversity and genotype multiplicity of infection in parasite isolates from cases with uncomplicated P. falciparum malaria in Southwest Ethiopia.Methods A total of 80 P. falciparum microscopy and qPCR positive blood samples were collected from study participants aged six months to sixty years, who visited the health facilities during study evaluating the efficacy of artemether-lumefantrine from September-December, 2017. Polymorphic regions of the msp-1 and msp-2 were genotyped by nested polymerase chain reactions (nPCR) followed by gel electrophoresis for fragment analysis.Results Of 80 qPCR-positive samples analysed for polymorphisms on msp-1 and msp-2 genes, the efficiency of msp-1 and msp-2 gene amplification reactions with family-specific primers were 95 % and 98.8%, respectively. Allelic variation of 90% (72/80) for msp-1 and 86.2% (69/80) for msp-2 were observed. K1 was the predominant msp-1 allelic family detected in 20.8% (15/72) of the samples followed by MAD20 and RO33. Within msp-2, allelic family FC27 showed a higher frequency (26.1%) compared to IC/3D7 (15.9%). Ten different alleles were observed in msp-1 with 6 alleles for K1, 3 alleles for MAD20 and 1 allele for RO33. In msp-2, 19 individual alleles were detected with 10 alleles for FC27 and 9 alleles for 3D7. Eighty percent (80%) of isolates had multiple genotypes and the overall mean multiplicity of infection was 3.2 (95% CI: 2.87- 3.46). The heterozygosity indices were 0.43 and 0.85 for msp-1 and msp-2, respectively. There was no significant association between multiplicity of infection and age or parasite density.Conclusions The study revealed high levels of genetic diversity and mixed-strain infections of P. falciparum populations in Chewaka district, Ethiopia, suggesting that both endemicity level and malaria transmission remain high and that strengthened control efforts are needed in Ethiopia.

show abstract

Genetic polymorphism of Merozoite Surface Protein-2 (MSP-2) in Plasmodium falciparum isolates from Pawe District, North West Ethiopia

Cited by 27 publications

References 28 publications

Genetic diversity of the msp-1, msp-2, and glurp genes of Plasmodium falciparum isolates in Northwest Ethiopia

Genetic diversity of the msp-1, msp-2, and glurp genes of Plasmodium falciparum isolates in Northwest Ethiopia

Seasonal variations in Plasmodium falciparum genetic diversity and multiplicity of infection in asymptomatic children living in southern Ghana

Genetic diversity and genotype multiplicity of Plasmodium falciparum infection in patients with uncomplicated malaria in Chewaka district, Ethiopia

Contact Info

Product

Resources

About