Genetic polymorphism of the 26 short tandem repeat loci in the Chinese Hebei Han population using two commercial forensic kits

Abstract

We determined the allele frequencies and forensic parameters for the 26 short tandem repeat (STR) autosomal markers in two commercial kits (the Investigator HDplex and AmpFLSTR(®) Identifiler(®) systems) for 183 unrelated individuals from the Han population of the Hebei Province of China. The 26 STRs were all in Hardy-Weinberg equilibrium. No linkage disequilibrium was detected between any pair of loci. The combined power of discrimination and the combined power of exclusion for the 26 STR loci were 1-7.74E-31 and 1-1.21E-11, respectively. Six rare alleles of D10S2325 were identified and named 20, 21, 22, 23, 24, and 31. All the length of the six rare alleles were out of the range of allelic ladder. We calculated the population pairwise genetic distance based on the allele frequencies, using published population data including German, central Polish, south Dutch, northeastern Polish, south Brazilian, Korean, Sichuan Han of China, and Shanghai Han of China. Also we examined the population pairwise genetic distance of loci included in Identifiler system between Hebei Han and other ethnic population of China. These 26 autosomal STR loci could provide highly informative polymorphic data for paternity testing and forensic identification in the Hebei Han population in China. Because they are all in linkage equilibrium, they could be used together to solve deficient kinship cases or cases with mutations.

Genetic polymorphism of 22 autosomal STR markers in a Han population of Southern China

“…Allelic frequencies for the twenty-two STRs were compared to the previously published Chinese Han population data estimated from southern China (Guangdong) [20], eastern China (Shanghai, Zhejiang) [19,21,22], northern China (Hebei) [23] and northwestern China (Chengdu) [24]. Only the frequencies for D18S535 were significantly different from the data from Shanghai (Table S4).…”

Expansion of a SNaPshot assay to a 55‐SNP multiplex: Assay enhancements, validation, and power in forensic science

“…All unrelated individuals genotyped using the 55plex panel were also genotyped using the AmpFℓSTR® Identifiler® kit (Applied Biosystems, USA) and the Investigator HDplex kit (Qiagen, Germany). Reactions and CE detection were performed as described in .…”

Genetic polymorphism of 29 STR loci in the Hunan Han population from China

“…Goldeneye TM 20A PCR kit was used following the manufacturer's guidelines [1]. Qiagen Investigator HDplex kit was used following the manufacturer ' s guidelines but reduce the volume to 10 mL [4]. PCR amplification was performed on a GeneAmp V R PCR 9700 thermal cycler (Thermo Fisher Scientific, Waltham, MA, USA).…”