Genetic polymorphism of the B subunit of coagulation factor XIII in Libyans: Occurrence of a fourth common allele, FXIIIB*6

Abstract

Summary. FXIIIB phenotypes were determined in neuraminidase-pretreated serum samples by using isoelectric focusing in ultrathin-layer polyacrylamide gels containing 1 M urea and subsequent immunoblotting. In a Libyan population sample from Tripoli, (n = 108) nine different phenotypes as products of four common alleles were recognized, with frequencies as follows: FXIIIB*I = 0.6574, FXIIIB*2 = 0.2454, FXIIIB*3 = 0.0741 and FXIIIB*6 = 0.0231. It is suggested that FXIIIB*6 is the fourth common allele of the FXIIIB system in this population.Key words: Coagulation factor XIIIB, Genetic polymorphism in LibyansAllele FXIIIB*6Zusammenfassung. Der genetisch determinierte Polymorphismus des FXIIIB wurde durch isoelektrische Fokussierung in ultradtinner Schicht eines Harnstoff-haltigen Polyacrylamidgels mit anschliegendem Immunoblott dargestellt. Die Serumproben wurden vor der Verimpfung mit Neuraminidase behandelt. Bei Libyern aus Tripoli (n = 108) wurden die neun h~iu-figen Phfinotypen FXIIIB 1-1, 2-1, 3-1, 2-2, 3-2, 3-3, 6-1, 6-3 und 6-6 beobachtet. Folgende Allelfrequenzen wurden ermittelt: FXIII*I = 0,6574, FXIIIB*2 = 0,2454, FXIIIB*3 = 0,0741 und FXIIIB*6 = 0,0231. Es wird vermutet, dab das Allel FXIIIB*6 das vierte, h~iufige (allgemeine) Allel in dieser Population darstellt.

Polymorphism of coagulation factor XIII B subunit: Further occurrence of FXIIIB*15 in Japanese and phenotyping in bloodstains

“…The bloodstains were stored at 37°C, room temperature and 4°C, and examined after different time intervals. The stains were cut in 5 x 6 mm pieces, treated with 10 gl neuraminidase (20 U/ml, Sigma) for 18 h and applied directly onto the gel surface.Isoelectric focusing was performed by the method of Sebetan and Azadeh [8]. Following electrofocusing, proteins were transferred onto a nitrocellulose membrane (Bio-Rad) by electroblotting.…”

Plasminogen Polymorphism in Libyans: Description of a New Rare Variant

“…The sera used in this investigation (n = 110) were from the same population sample which was previ ously analyzed for the genetic polymorphisms of otr HS-glycoprotein, orosomucoid (ORM1 and ORM2) and coagulation factor XIII B [3][4][5]. Neur aminidase treatment of the sera, isoelectric focusing and immunoblotting were according to our earlier study [6].…”

Allele‐specific alternative splicing; the tail of FXIII‐B tells its own tale

“…Such clear segregation of the three alleles over three continents is a rather unique genetic feature of FXIII‐B. Several other minor alleles have also been described, including B*4, B*6, B*13, B*14 and B*15, but especially the latter alleles are relatively rare [17–19]. The genetic variation of FXIII‐B is responsible for some of the population variability in FXIII activity, with the highest activity observed for FXIII‐B*2, followed by FXIII‐B*1 and the lowest activity for FXIII‐B*3 [20].…”