Genetic polymorphism of the kappa-casein gene in Brazilian cattle

Azevedo, Ana Luisa Sousa; Nascimento, Carlos Souza do; Steinberg, Raphael S.; Carvalho, Maria Raquel Santos; Peixoto, Maria Gabriela Campolina Diniz; Teodoro, R. L.; Verneque, R.S.; Guimarães, Simone Eliza Facioni; Machado, M. A.

doi:10.4238/vol7-3gmr428

Cited by 60 publications

(53 citation statements)

References 22 publications

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“…K-Ca 'B' allele was reported to have a favorable and significant effect on both milk yield and milk protein content in Reggiana dairy local cattle , different Indian buffaloes breeds (Raj et al, 2008), nili-ravi buffalo (Riaz et al, 2008). Also, its favorable effect on milk technological properties and cheese yield in taurine and zebu cattle breeds (Ceriotti et al, 2004) and Brazilian cattle (Azevedo et al, 2008), desirable coagulation properties reported in Finnish Ayrshire cows (Ikonen et al, 1997). …”

Section: Introductionmentioning

confidence: 99%

Genetic Variants and Allele Frequencies of Kappa Casein in Egyptian Cattle and Buffalo Using PCR-RFLP

Gouda¹,

Galal²,

Abdel‐Aziz³

2013

JAS

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Kappa casein (K-Ca) genetic variations affected quality and composition of the milk. Several variants of Kappa casein (K-Ca) gene locus IV have been reported with special interest for the 'B' allele for its relation to the milk protein and fat yields. Genotyping and allelic frequencies of K-Ca among Native Egyptian breeds of cattle and buffalo were the aim of the present study. PCR amplification of DNA isolated from 300 blood samples collected from Holstein and Baladi cattle and buffalo were performed followed by restriction fragment length polymorphism using Hind-III restriction endonuclease (PCR-RFLP). Detection of 'AA' and 'AB' genotypes in cattle breeds, 'BB' and 'AB' in buffalo and two alleles 'A' and 'B' in the studied breeds. Molecular selection for animals carrying the 'B' allele could impact breeding programs for dairy production in native cattle and buffalo breeds in Egypt.

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Section: Introductionmentioning

confidence: 99%

Genetic Variants and Allele Frequencies of Kappa Casein in Egyptian Cattle and Buffalo Using PCR-RFLP

Gouda¹,

Galal²,

Abdel‐Aziz³

2013

JAS

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“…The PCR-RFLP technique has been used to study the frequency of the κ-Cn genotypes (alleles) in bovines as well as for dairy ca le (Alipanah et al, 2005;Azevedo et al, 2008). Identifi cation of the variant genotypes of the κ-Cn gene in this study was also performed through the PCR-RFLP method.…”

Section: Amplifi Cationmentioning

confidence: 99%

“…The overall length of the κ-Cn gene is close to 13 kb, in the chromosome 6q31. The κ-Cn is considerably diff erent from other caseins in structure and other properties (Azevedo et al, 2008). The genetic polymorphism of the κ-Cn gene in ruminant has been well documented providing ca le (Pinders et al, 1991;Rĳ njkels et al, 1997), buff aloes (Pipalia et al, 2001;Otaviano et al, 2005;Patel et al, 2007;Riaz et al, 2008), goats (Angiolillo et al, 2002;Yahyaoui et al, 2003;Prinzenberg et al, 2005;Kiplagat et al, 2010), sheep (Moioli et al, 2007) and yak (Prinzenberg et al, 2008).…”

Section: Kappa-casein Genotypic Frequencies Inmentioning

confidence: 99%

Kappa-Casein Genotypic Frequencies in Holstein-Friesian Dairy Cattle in West Java Province

Anggraini¹,

Sumantri²,

Farajallah³

et al. 2010

medpet

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Blood samples were also taken via cocsigalis vein of AI (active and non active) HF bulls at the two National AI Stations in Lembang (25 hds) and Singosari (32 hds). Identifi cation of genetic variationwas by applying PCR-RFLP method. Three genotypes were identifi ed, namely AA, AB, and BB, resulting two alleles of A and B. Results entirely showed that the frequencies of the BB HF female were very low, with the range of 0%-6% (vs AA genotype= 10%-54% and AB genotype= 46%-85%), despite of the relatively high frequency of the B allele over the A allele (23%-48% vs. 52%-77%). The low frequency of the observed BB females might be due to the limited AI active BB bulls used for services by the two national AI stations (0%-4%).

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“…These animals had already been genotyped for κ-casein polymorphism by PCR-RFLP with the restriction enzyme HinfI (Azevedo et al, 2008;Peixoto et al, 2012).…”

Section: Samplesmentioning

confidence: 99%

“…The κ-casein allele A has been associated with lower milk fat and calcium amount and a lower milk pH (Kürbarsepp et al, 2005). However, allele B is rare in indicine breeds selected for milk production and has not been found in meat selection zebu herds (Kemenes et al, 1999;Azevedo et al, 2008). To date, no report on the effects of this polymorphism on milk coagulation properties in cheese production in indicine breeds have been published.…”

Section: Introductionmentioning

confidence: 99%

A new tetra-primer ARMS-PCR for genotyping bovine kappa-casein polymorphisms

Fonseca

Rosse²,

Demiranda³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Kappa-casein (κ-casein) is one of the most abundant milk proteins. Its main function is to avoid the aggregation of casein micelles, keeping them, and therefore calcium phosphate, in pockets in solution. In bovines, a κ-casein functional polymorphism has been associated with fat, calcium, and protein milk contents and faster curd contraction in cheese production. Quicker curd contraction reduces the loss of milk solids, enhancing cheese yield. This polymorphism induces a double amino acid substitution (Thr136Ile and Ala148Asp). The polymorphism is normally detected by PCR-RFLP, which is a laborious method. An interesting methodological alternative is the tetra-primer amplification refractory mutation system PCR (tetra-primer ARMS-PCR). A tetraprimer ARMS-PCR for the detection of this κ-casein polymorphism has been described. However, specificity was not achieved, probably due to problems with primer design. We developed a new tetra-primer ARMS-PCR for the detection of the κ-casein polymorphism. This new method was validated in a double-blind test, by comparison with the results obtained for 50 Guzerá bulls formerly genotyped by PCR-RFLP. This new method achieved 100% sensitivity and specificity. We conclude that this method is a useful, cost-efficient alternative for the detection of functional κ-casein polymorphisms.

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Genetic polymorphism of the kappa-casein gene in Brazilian cattle

Cited by 60 publications

References 22 publications

Genetic Variants and Allele Frequencies of Kappa Casein in Egyptian Cattle and Buffalo Using PCR-RFLP

Genetic Variants and Allele Frequencies of Kappa Casein in Egyptian Cattle and Buffalo Using PCR-RFLP

Kappa-Casein Genotypic Frequencies in Holstein-Friesian Dairy Cattle in West Java Province

A new tetra-primer ARMS-PCR for genotyping bovine kappa-casein polymorphisms

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