Genetic polymorphisms among human cytomegalovirus (HCMV) wild‐type strains

Pignatelli, Sara; Monte, Paola Dal; Rossini, Giada; Landini, Maria Paola

doi:10.1002/rmv.438

Cited by 134 publications

(131 citation statements)

References 147 publications

(293 reference statements)

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“…Multiple strains of virus circulate both within the population and within an individual, and evidence exists for inter-strain recombination [18][19]. There are no data on the genetic variants of HCMV in IBD.…”

Section: Discussionmentioning

confidence: 99%

Toxic megacolon and human Cytomegalovirus in a series of severe ulcerative colitis patients

Criscuoli

Rizzuto

Gallo

et al. 2015

Journal of Clinical Virology

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a b s t r a c tBackground: Human Cytomegalovirus (HCMV) infection has been reported to be a cause of refractory ulcerative colitis (UC). Toxic megacolon (TM) is a rare but severe complication of an acute attack of UC. Objectives: Aim of this study is to evaluate in a case-control study the association between HCMV and TM. Study design: All patients who were admitted at Medicine Department of V. Cervello Hospital in Palermo (tertiary referral center) for a severe UC flare-up complicated by the onset of TM (diameter of the transverse colon > 6 cm) between January 1990 and November 2011 were identified through the electronic database. A total of 24 consecutive patients (16 male/8 female) with TM were identified. Each case of TM were individually matched by sex, age, extent of the underlying disease to 24 severe UC controls who did not develop TM. A further non matched control population of 48 severe UC was included.Haematoxilin and eosin stain, immunohistochemical procedure and nested polymerase chain reaction were performed to detect HCMV genes and proteins on rectal biopsies or surgical specimens. Pp65 antigenemia was performed in order to diagnose any possible systemic infection. HCMV frequency was compared between patients with and without TM during follow-up, using Fisher's Exact test. Results and conclusions: HCMV was detected in histological specimens of 11 patients (46%) with TM compared to 2 (9%) severe UC matched controls (P = 0.0078) and 7 (14%) unmatched controls (p = 0,003). In severe colitis the presence of HCMV is more frequently associated with TM.

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Section: Discussionmentioning

confidence: 99%

Toxic megacolon and human Cytomegalovirus in a series of severe ulcerative colitis patients

Criscuoli

Rizzuto

Gallo

et al. 2015

Journal of Clinical Virology

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“…Genotypic differences in the CMV envelope proteins, such as gB, have been proposed as markers of CMV pathogenicity; these can help to detect mixed infections, and to establish the genotypic similarities among viruses identified in different samples from a single patient [9,10].…”

Section: Introductionmentioning

confidence: 99%

Detection and gB genotyping of CMV in Mexican preterm infants in the context of maternal seropositivity

Arellano‐Galindo

Villanueva-García

Cruz-Ramirez

et al. 2014

J Infect Dev Ctries

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Introduction: Congenital (CI) and perinatal cytomegalovirus (CMV) infections (PI) can be linked to maternal CMV seropositivity, with fatal consequences in preterm newborns. GB genotyping has been used to analyze genotypic similarity in mothers and infants. The frequency of CMV infection in the context of maternal seropositivity and the viral gB genotypes as well as the genotypic similarity in mothers and preterm infants were investigated. Methodology: Saliva samples and dry blood spots (DBS) were taken weekly from preterm newborns from birth until the first month of life, and breast milk samples were taken from their mothers weekly during the first month of lactation. CMV IgG seroprevalence of the mothers and CI or PI in the infants were established. The gB status and genotypic similarities were established retrospectively in DBS and in the breast milk samples. Results: In total, 387 neonates and 375 mothers were enrolled. The maternal CMV-positive IgG serology was 97.3% (365/375). Neonatal CMV was found in 5.1% (20/387) of newborns, and one infant presented with CMV-compatible symptoms. CI was 2.5% and PI in the first month after birth was 11.8%. GB2 was the most prevalent genotype and was also the genotype preferentially transmitted to newborns by mothers with mixed infections. Conclusions: CMV PI and CI in preterm infants from highly seropositive mothers was high, but the rate of symptomatic infection was low. The prevalent genotype was gB2, and this genotype was preferentially transmitted to newborns by mothers with mixed infections.

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“…The variable region was located in the N-terminal region of protein. The clustered polymorphisms in the UL132 sequence imply that selective pressures might favor retention of each group, as proposed for other glycoprotein ORFs (24,26). Other selective forces, such as viral cell tropism, virulence, stability and complex formation may induce the accumulation of specific viral variants with a higher fitness in a given selective environment.…”

Section: Discussionmentioning

confidence: 89%

Genetic Variability of Human Cytomegalovirus UL132 Gene in Strains from Infected Infants

Sun

Yao

Ruan

et al. 2006

Microbiology and Immunology

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Genetic polymorphisms among human cytomegalovirus (HCMV) wild‐type strains

Cited by 134 publications

References 147 publications

Toxic megacolon and human Cytomegalovirus in a series of severe ulcerative colitis patients

Toxic megacolon and human Cytomegalovirus in a series of severe ulcerative colitis patients

Detection and gB genotyping of CMV in Mexican preterm infants in the context of maternal seropositivity

Genetic Variability of Human Cytomegalovirus UL132 Gene in Strains from Infected Infants

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