Genetic polymorphisms in 12 autosomal STRs in a Shanghai Han population from China

Abstract

In this study, a total of 484 unrelated healthy individuals and 359 two-generation families in the Han population in Shanghai, China were successfully analyzed with the Investigator HDplex Kit (Qiagen, Hilden, Germany). Hardy-Weinberg equilibrium tests demonstrated no significant deviation from expected values (p > 0.05) for the 12 autosomal STRs included in this kit. A total of 173 alleles were detected in 12 autosomal STR loci; the largest number of alleles (32) was detected for the SE33 locus. The combined power of discrimination was 0.99999999992. The combined mean exclusion chance in duo cases was 0.99982, whereas the combined mean exclusion chance in trio cases was 0.9999986. The average mutation rate across all loci was 0.0023 (95% confidence intervals: 0.0013-0.0039). These results suggest that the 12 autosomal STR loci can provide highly informative polymorphic data for paternity testing and forensic identification in the Han population in Shanghai, China.

Genetic polymorphism of 22 autosomal STR markers in a Han population of Southern China

“…Hence, we present the mapping positions and genetic locations of the 22 STR loci, as well as common forensic loci on the same chromosome, in Table S3. In addition, the mutation rates of 7 out of the 22 STRs have been reported in references [18] and [19]. Their results indicated that the mutation rates were similar to those of commonly used forensic STR loci.…”

“…Allelic frequencies for the twenty-two STRs were compared to the previously published Chinese Han population data estimated from southern China (Guangdong) [20], eastern China (Shanghai, Zhejiang) [19,21,22], northern China (Hebei) [23] and northwestern China (Chengdu) [24]. Only the frequencies for D18S535 were significantly different from the data from Shanghai (Table S4).…”

Development and validation of a novel SiFaSTRTM 23‐plex system

“…PCR primer designing of each locus was performed using the primer premier v5.0 and Oligo v6.0 by applying the following main criteria: primer length of 18–35 base pair (bp); PCR amplicon length ranging from 60 to 500 bp; Tm values ranging from 57 to 68°C (best approximately 60°C), and an optimum GC content of 40–60%. The allelic data presented in the STRBase web (http://www.cstl.nist.gov/div831/strbase/) and previously studied in our laboratory were also referenced during the design of the primers. The obtained primer pairs were checked for non‐specific hybridizations in other genome regions using the Basic Local Alignment Search Tool (BLAST) in NCBI at http://blast.ncbi.nlm.nih.gov/.…”

Evaluation of forensic genetic parameters of 12 STR loci in the Korean population using the InvestigatorⓇ HDplex kit

“…Forensic genetic studies using the Investigator Ⓡ HDplex kit have been performed in different laboratories [2,[4][5][6][7][8]. To date, the use of the Investigator Ⓡ HDplex kit to calculate the forensic genetic parameters of the Korean population has not been reported.…”

“…Calculations of allele frequencies, observed and expected heterozygosity, the values of Fst, and pairwise p values for the pairwise comparison of genetic distances in Korean and other populations were performed using Arlequin v3.5. Allele frequencies were obtained from published population data including Argentinian [2], North Italian [4], Dutch [6], Japanese [7], and Shanghai Han [8] populations. The population genetics parameters including matching probability (PM), power of discrimination (PD), polymorphism information content (PIC), and power of exclusion (PE) were calculated using PowerStats v1.…”