Genetic polymorphisms in bovine transferrin receptor 2 (TFR2) and solute carrier family 40 (iron‐regulated transporter), member 1 (SLC40A1) genes and their association with beef iron content

Abstract

SummaryBeef is considered to be an excellent source of dietary iron. However, little is known about the genetic control of beef iron content. We hypothesized that genetic polymorphisms in transferrin receptor 2 (TFR2) and solute carrier family 40 (iron‐regulated transporter), member 1 (SLC40A1) could influence skeletal muscle iron content. The objective of this study was to use Angus cattle to identify single‐nucleotide polymorphisms (SNPs) in the exons and flanking regions of the bovine TFR2 and SLC40A1 genes and to evaluate the extent to which genetic variation in them was associated with bovine longissimus dorsi muscle iron content. Ten novel SNPs were identified in TFR2, of which one SNP tended to be associated (P < 0.013) with skeletal muscle iron content. Nine novel SNPs in SLC40A1, NC007300: rs133108154, rs137140497, rs135205621, rs136600836, rs134388440, rs136347850, rs134186279, rs134621419 and rs137555693, were identified, of which SNPs rs134388440, rs136347850 and rs137555693 were significantly associated (P < 0.007) with skeletal muscle iron content. High linkage disequilibrium was observed among SLC40A1 SNPs rs134388440, rs136347850 and rs137555693 (R2 > 0.99), from which two haplotypes, TGC and CAT, were defined. Beef from individuals that were homozygous for the TGC haplotype had significantly (P < 0.001) higher iron content than did beef from CAT homozygous or heterozygous individuals. The estimated size of effect of the identified haplotypes was 0.3% of the phenotypic variance. In conclusion, our study provides evidence for genetic control of beef iron concentration. Moreover, SNPs identified in SLC40A1, rs134388440, rs136347850 and rs137555693 might be useful markers for the selection of Angus cattle for altered iron content.

Sire breed effect on beef longissimus mineral concentrations and their relationships with carcass and palatability traits

“…Liver zinc concentration, however, was different between Limousin and Pinzgauer cattle, but not among the other breeds. While we did not identify breed effects on these five mineral concentrations within LT, it is possible for mineral concentrations to be influenced by genetics which vary within breed (Casas et al, 2014;Duan et al, 2011;. In a large study in lambs, Pannier et al (2014) identified significant effects of sire type on iron (P b 0.01) and zinc (P b 0.05) concentrations in LT.…”

“…Beef mineral concentrations vary among individuals and are affected by various physiological, environmental, and within breed additive genetic factors (Doyle, 1980;Duan et al, 2011;Zarkadas et al, 1987). Few studies have evaluated mineral concentrations across several sire and dam breeds of cattle.…”

A Multiple-SNP Approach for Genome-Wide Association Study of Milk Production Traits in Chinese Holstein Cattle

“…SLC40A1 encodes ferroportin which is an iron transporter involved in iron export from cell, and it is essential for iron homeostasis. Moreover, Duan et al found that the polymorphisms of this gene as well as TRF2 (transferrin receptor 2) were significantly associated with beef iron concentration [16]. It is possible that the polymorphisms of SLC40A1 have an effect on iron homeostasis of bovine breast cells, which leads to a potential relationship with milk yield.…”

An interpretive review of selective sweep studies in Bos taurus cattle populations: identification of unique and shared selection signals across breeds

“…As a result of the large size of this CSS, selective sweeps that originally did not include the GDF -8 gene have been incorporated under the CSS-32 label; these include two sweeps described in a Piedmontese vs. Italian Brown comparison (Pintus et al, 2014 ) 6.717–9.760 Mb, including the SLC40A1 (solute carrier family 40, member 1), COL5A2 (collagen, type V, alpha 2), COL3A1 (collagen, type III, alpha 1), CALCRL (calcitonin receptor-like) and ITGAV (integrin, alpha V) genes, and a Fleckvieh selective sweep located in a gene-desert region. Polymorphisms in the SLC40A1 gene have been related to beef iron content (Duan et al, 2012 ).…”