Genetic polymorphisms of 17 short tandem repeat loci on Y chromosome in central Croatian population

Abstract

In forensic casework, Y-chromosome short tandem repeat (STR) haplotyping is used in human identification, paternity testing and sexual assault cases where Y-STRs provide a male-specific DNA profile. The aim of this study was to describe the genetic structure of Y chromosome in a central Croatian population. We carried out a statistical analysis of the data from previously performed genetic analyses collected during routine forensic work by the Forensic Science Centre "Ivan Vučetić". A total of 220 unrelated healthy men from central Croatia were selected for the purpose of this study. Genomic DNA was extracted using a Chelex procedure from FTA(®) cards. Y-chromosomal STRs were determined using the AmpFISTR Yfiler PCR amplification kit. The haplotype frequencies were determined by direct counting and analyzed using Arlequin 3.1 and analysis of molecular variance calculated with the Y chromosome haplotype reference database online analysis tool. A total of 212 haplotypes were identified, 204 of which were unique. Total haplotype diversity was 0.993. Locus diversity varied from 0.325 for DYS392 to 0.786 for DYS385. Discrimination capacity was 92.7%. Allele frequencies diversity was 0.615. Intermediate alleles 17.2, 18.2 and 19.2 were found at DYS458 locus. A comparison with published data for the European minimal haplotype set showed the closest relationship to the Croatian capital of Zagreb and Bosnia and Herzegovina with significant genetic distance from Slovenia and Austria. The central Croatian population is now well characterized in terms of Y-chromosome STRs, thus providing a solid basis for further forensic and genetic epidemiology studies.

Prenatal diagnosis of single gene disorders using amniotic fluid as the starting material for PCR

“…The Chelex method was developed for extracting DNA from many different types of samples, including whole blood, bloodstains, hair, and AF, for use in PCR, and especially for paternity testing. 28,29 Although DNA extraction using Chelex 100 is as efficient, or more efficient, than methods using proteinase K and phenol-chloroform extraction, 30,31 the Chelex 100 method requires multiple steps for DNA extraction, which can result in cross-contamination and is costly. In our previous work, 18 we proposed a method using AF as the starting material for PCR to overcome the drawbacks of other extraction methods.…”

The impact of 2011!

“…Postmortem and disaster victim identification were included this year with an analysis of the use of permanent maxillary molar cusp areas in sex determination [15] and in a comparison of various methods for age determination in child victims of the South East Asian tsunami [16]. Genetic polymorphisms on the Y chromosome in a central Croatian population were also reported [17].…”