Genetic polymorphisms of vascular endothelial growth factor in severe pre-eclampsia

Abstract

Several lines of evidence support the hypothesis that vascular endothelial growth factor (VEGF) plays an important role in the pathogenesis of pre-eclampsia (PE). VEGF is a key component in the regulation of vascular remodelling and the survival of cytotrophoblasts in the placenta. In this case-control study, we aimed to test whether VEGF genetic polymorphisms are associated with the risk of severe PE. We enrolled 84 nulliparous pregnant women with severe PE (PE group). Their VEGF G(+405)C and VEGF C(-2578)A genotypes were determined by PCR-restriction fragment length polymorphism (PCR-RFLP) from venous blood samples and were compared with the corresponding VEGF genotypes of 96 nulliparous patients with uncomplicated pregnancies (control group). Carriers of the VEGF(+405)G allele occurred less frequently in PE than in the control group [P = 0.039; adjusted odds ratio (aOR) = 0.28, range: 0.08-0.93]. Hypertension and proteinuria were diagnosed earlier (by 1.6 weeks and 1.9 weeks, respectively) in PE patients with VEGF(-2578)A only after adjustment of this association for risk factors of PE. Our results suggest that carriers of VEGF(+405)G allele have a decreased susceptibility to PE and that the progression of PE may be modified by the presence of VEGF(-2578)A allele. Nevertheless, the clinical significance of these findings remains to be determined.

Searching for Genes That Matter in Acute Kidney Injury

“…In the HSP72 gene, Fekete et al (31) found that VLBW infants homozygous for the G allele were at increased risk for AKI (OR ϭ 3.17, P Ͻ 0.01). This same group also found that for the VEGF Ϫ2578 C/A polymorphism, VLBW infants homozygous for the A allele were protected against AKI (OR ϭ 0.2, P ϭ 0.021) (29).…”

Polymorphisms in renin-angiotensin-aldosterone system and vascular endothelial growth factor may cross talk in preeclampsia: a pilot study of maternal and fetal dyads in Indian population

“…4 Analysis of genetic variations of fetuses showed two SNPs with significant difference 1) RENIN (rs11240688) (Chi-square= 9.673, p=0.002) with OR=3.042 (CI is 1.476 to 6.267).…”

“…Banyasz et al found carriers of allele VEGF G405C occurred less frequently in pre-eclampsia than in controls and that the progression of preeclampsia may be modified by the presence of VEGF 2578A allele. 4 Shim et al (2007) evaluated the distribution of +936 C/T polymorphism in VEGF gene in Korean women and found that its carriage may be associated with increased susceptibility to the development of PE and may be an independent risk factor. 5 Preeclampsia presents a peculiar challenge, in that both the maternal and fetal genotype may contribute to the clinical phenotype.…”

NETWORK APPROACH TO ANALYSIS OF QUANTITATIVE TRAIT LOCI FOR TUMOR NECROSIS FACTOR (TNFα-863, TNFα-308, TNFα-238), VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF-2578, VEGF+936) AND MATRIX METALLOPROTEINASE (Ммр2-1306, Ммр3-1171, Ммр9-1569) GENES IN AGERELATED MACULAR DEGENERATION

“…Генотипирование Генотипирование полиморфизмов промо-торного региона генов VEGF -2578 (rs 699947), ММР3-1171 (rs 3025058), ММР9-1562 (rs 3918242), TNFα-863 (rs1800630), TNFα-308 (rs1800629), TNFα-238 (rs 361525) осуществляли методом ре-стриктазного анализа продуктов амплификации (ПДРФ-анализ), с использованием специфич-ных праймеров [5,6,21,26,29] Статистический анализ При статистическом анализе результатов ис-следований использовали такие показатели, как частота встречаемости генотипов, отноше-ние шансов (OR) с расчетом 95% доверительного интервала (OR 95%CI). Расчет величины OR про-водили по методу Вульфа-Холдейна [1].…”