Genetic positioning of Korean viral hemorrhagic septicemia virus (VHSV) from cultured and wild marine fishes

Kim, Wi Sik; Jung, Sunyo; Kim, Jong Oh; Kim, Du Woon; Kim, Jeong-Ho; Oh, Myung Joo

doi:10.7847/jfp.2011.24.1.001

Cited by 20 publications

(14 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, several pathogenic fish viruses have been isolated or detected from wild marine fish in coastal areas of Korea. 4,10,11 Thus, feeding virus-infected wild marine fish to domestic stock is considered to be a potential route of viral infection. 23,25 A survey of fish viruses from wild marine fishes in coastal areas can provide significant information.…”

mentioning

confidence: 99%

A survey of fish viruses isolated from wild marine fishes from the coastal waters of southern Korea

et al. 2013

Self Cite

View full text Add to dashboard Cite

Abstract.A survey was conducted to investigate viral infection in 253 wild marine fishes harvested in the southern coastal area of Korea from 2010 to 2012. The fish that were captured by local anglers were randomly bought and sampled for virus examination. The samples were tested for presence of virus by virus isolation with FHM, FSP, and BF-2 cells and molecular methods (polymerase chain reaction and sequencing). Of the 253 fish sampled, 9 fish were infected with virus. Aquabirnaviruses (ABVs), Viral hemorrhagic septicemia virus (VHSV), and Red seabream iridovirus (RSIV) were detected in 7, 1, and 1 fish, respectively. Molecular phylogenies demonstrated the detected viruses (ABV, VHSV, and RSIV) were more closely related to viruses reported of the same type from Korea and Japan than from other countries, suggesting these viruses may be indigenous to Korean and Japanese coastal waters.

show abstract

mentioning

confidence: 99%

A survey of fish viruses isolated from wild marine fishes from the coastal waters of southern Korea

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…In Far East Asia, VHSV was first isolated from free-living olive flounder Paralichthys olivaceus in 1999 (Takano et al, 2000). Since then, VHSV-infection with severe mortality has occurred frequently at olive flounder farms in Korea and Japan (Isshiki et al, 2001;Kim et al, 2009) and VHSV has isolated from several wild marine fish species obtained on both Korean and Japanese coasts (Watanabe et al, 2002;Kim et al, 2011a).…”

Section: Viral Hemorrhagic Septicaemia (Vhs) Is An Infectiousmentioning

confidence: 99%

“…VHSV is a member of the genus Novirhabdovirus from the family Rhabdoviridae (Trdo et al, 2005), and classified into four genotypes I-IV (Benmansour et al, 1997;Stone et al, 1997;Nishizawa et al, 2002;EinerJensen et al, 2004;Elsayed et al, 2006;Gagné et al, 2007;Kim et al, 2011a So far, VHSV has not been isolated and/or detected from salmonids including rainbow trout in Korea, and mariculture for rainbow trout and masu salmon O. masou has been initiated on the Korean coast near olive flounder farms. Although it was previously reported that Asian VHSV isolates from olive flounder were pathogenic to rainbow trout fry (Kim et al, 2011b), pathogenicity of VHSV to masu salmon is remained unclear.…”

Section: Viral Hemorrhagic Septicaemia (Vhs) Is An Infectiousmentioning

confidence: 99%

Pathogenicity of viral hemorrhagic septicemia virus (VHSV) isolated from olive flounder Paralichthys olivaceus to masu salmon Oncorhynchus masou

Kim¹,

Kim

Nishizawa³

et al. 2012

Journal of fish pathology

Self Cite

View full text Add to dashboard Cite

The pathogenicity of viral hemorrhagic septicemia virus (VHSV) from olive flounder Paralichthys olivaceus was investigated with masu salmon Oncorhynchus masou fry. The cumulative mortality of fish challenged with FYeosu05 isolate at 10 6.5 TCID50/fish was 60%. No mortality was observed in fish challenged with the isolates at 10 5.5 TCID50/fish and in mock-challenged fish. The affected fish showed darkening of the body, expanded abdomen, pale gills and enlarged spleen. VHSV from 10 6.3 to 10 7.8 TCID50/g-tissue was re-isolated from the dead fish. These results suggest that the VHSV from olive flounder is pathogenic to masu salmon fry, although with low virulence.

show abstract

“…As olive flounder ( Paralichthys olivaceus ) is one of the preferred food sources in Korea, there has been an increase in the number of olive flounder farms. However, many farms have suffered substantial financial loss due to the occurrence of viral hemorrhagic disease (VHS) [1,2,3]. …”

Section: Introductionmentioning

confidence: 99%

Development and Application of Quantitative Detection Method for Viral Hemorrhagic Septicemia Virus (VHSV) Genogroup IVa

Kim

et al. 2014

Viruses

Self Cite

View full text Add to dashboard Cite

Viral hemorrhagic septicemia virus (VHSV) is a problematic pathogen in olive flounder (Paralichthys olivaceus) aquaculture farms in Korea. Thus, it is necessary to develop a rapid and accurate diagnostic method to detect this virus. We developed a quantitative RT-PCR (qRT-PCR) method based on the nucleocapsid (N) gene sequence of Korean VHSV isolate (Genogroup IVa). The slope and R2 values of the primer set developed in this study were −0.2928 (96% efficiency) and 0.9979, respectively. Its comparison with viral infectivity calculated by traditional quantifying method (TCID50) showed a similar pattern of kinetic changes in vitro and in vivo. The qRT-PCR method reduced detection time compared to that of TCID50, making it a very useful tool for VHSV diagnosis.

show abstract

Genetic positioning of Korean viral hemorrhagic septicemia virus (VHSV) from cultured and wild marine fishes

Abstract: Viral haemorrhagic septicaemia virus (VHSV) is an epidemic virus in olive flounder

Cited by 20 publications

References 21 publications

A survey of fish viruses isolated from wild marine fishes from the coastal waters of southern Korea

A survey of fish viruses isolated from wild marine fishes from the coastal waters of southern Korea

Pathogenicity of viral hemorrhagic septicemia virus (VHSV) isolated from olive flounder Paralichthys olivaceus to masu salmon Oncorhynchus masou

Development and Application of Quantitative Detection Method for Viral Hemorrhagic Septicemia Virus (VHSV) Genogroup IVa

Contact Info

Product

Resources

About