Genetic Regulation of Endothelial Inflammatory Responses in Baboons

Rainwater, David L.; Shi, Qiang; Mahaney, Michael C.; Hodara, Vida; VandeBerg, John L.; Wang, Xing Li

doi:10.1161/atvbaha.110.205740

Cited by 14 publications

(13 citation statements)

References 44 publications

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“…The expression of proportions of cell specific cellular adhesion molecules was quantified by standard immunofluorescence cell sorting techniques [19, 20]. The following antibodies were used in this study: anti-human CD62E (clone BBIG-E5), anti-human CD54 (clone HA58), and anti-human CD106 (clone 5K267).…”

Section: Methodsmentioning

confidence: 99%

Protective Effects of Resveratrol on TNF-α-Induced Endothelial Cytotoxicity in Baboon Femoral Arterial Endothelial Cells

Xiao

Song

Hodara

et al. 2013

Journal of Diabetes Research

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Endothelial injury induced by inflammatory factors plays a critical role in the pathogenesis of cardiovascular disease. Endothelial cell (EC) apoptosis, proliferation, migration, and cellular adhesion molecule (CAM) expression contribute to the development of atherosclerosis. We investigated the effects of resveratrol (0.1–100 μM) on the proliferation, migration, and CAM expression of primary cultures of baboon arterial endothelial cells (BAECs). In addition, we tested its effects under normal conditions as well as under inflammatory conditions induced by tumour necrosis factor-α (TNF-α) administered either by cotreatment, pretreatment, or posttreatment. Immunocytochemistry, MTT, wound-healing, and flow cytometry assays were performed. The resveratrol treatment significantly enhanced BAEC proliferation and attenuated TNF-α-induced impairment of proliferation at the optimal doses of 1–50 µM. Resveratrol at a high dose (100 μM) and TNF-α impaired BAEC migration, while low doses of resveratrol (1–50 μM) attenuated TNF-α-induced impairment of BAEC migration. Moreover, resveratrol inhibited TNF-α-induced ICAM-1 and VCAM-1 expression. Taken together, our results suggest that the resveratrol protects BAECs after inflammatory stimulation as well as ameliorates inflammatory effects at low concentrations. Consequently, resveratrol should be considered as a candidate drug for the prevention and treatment of inflammatory vascular diseases.

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Section: Methodsmentioning

confidence: 99%

Protective Effects of Resveratrol on TNF-α-Induced Endothelial Cytotoxicity in Baboon Femoral Arterial Endothelial Cells

Xiao

Song

Hodara

et al. 2013

Journal of Diabetes Research

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“…Some responders exhibit a large HDL 1 lipoprotein when fed a saturated fat diet and there appears to be a negative correlation between the extent of atherosclerosis and HDL 1 levels. The analysis of members of a pedigree from crosses between olive and yellow baboons showed that the expression of arterial endothelial cell adhesion molecules in response to TNFα stimulation was highly heritable (52). The size, the expense of their upkeep and the length of time required for development of atherosclerosis, as well as the difficulties of genetic studies, have limited the utility of non-human primates for atherogenesis research.…”

Section: Animal Models Of Atherosclerosismentioning

confidence: 99%

Animal Models of Atherosclerosis

Getz

Reardon

2012

ATVB

521

357

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Atherosclerosis is a chronic inflammatory disorder that is the underlying cause of most cardiovascular disease. Both cells of the vessel wall and cells of the immune system participate in atherogenesis. This process is heavily influenced by plasma lipoproteins, genetics and the hemodynamics of the blood flow in the artery. A variety of small and large animal models have been used to study the atherogenic process. No model is ideal as each has its own advantages and limitations with respect to manipulation of the atherogenic process and modeling human atherosclerosis or lipoprotein profile. Useful large animal models include pigs, rabbits and non-human primates. Due in large part to the relative ease of genetic manipulation and the relatively short time frame for the development of atherosclerosis, murine models are currently the most extensively used. While not all aspects of murine atherosclerosis are identical to humans, studies using murine models have suggested potential biological processes and interactions that underlie this process. As it becomes clear that different factors may influence different stages of lesion development, the use of mouse models with the ability to turn on or delete proteins or cells in tissue specific and temporal manner will be very valuable.

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“…While IL-1a and VCAM were expressed to a lower level after DC, the expression of IL-8 increased after DC. Several studies have shown a correlation between IL-8 expression, arteriosclerosis and coagulation which is thought to be caused by monocyte activation, adhesion and transmigration across the endothelial barrier [34,38]. However, not only shear stress, but rather cell isolation from biopsies provokes stress symptoms.…”

Section: Discussionmentioning

confidence: 99%

Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering

Aleksieva¹,

Hollweck²,

Thierfelder³

et al. 2012

BioMed Eng OnLine

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BackgroundTissue engineering represents a promising new method for treating heart valve diseases. The aim of this study was evaluate the importance of conditioning procedures of tissue engineered polyurethane heart valve prostheses by the comparison of static and dynamic cultivation methods.MethodsHuman vascular endothelial cells (ECs) and fibroblasts (FBs) were obtained from saphenous vein segments. Polyurethane scaffolds (n = 10) were primarily seeded with FBs and subsequently with ECs, followed by different cultivation methods of cell layers (A: static, B: dynamic). Group A was statically cultivated for 6 days. Group B was exposed to low flow conditions (t1= 3 days at 750 ml/min, t2= 2 days at 1100 ml/min) in a newly developed conditioning bioreactor. Samples were taken after static and dynamic cultivation and were analyzed by scanning electron microscopy (SEM), immunohistochemistry (IHC), and real time polymerase chain reaction (RT-PCR).ResultsSEM results showed a high density of adherent cells on the surface valves from both groups. However, better cell distribution and cell behavior was detected in Group B. IHC staining against CD31 and TE-7 revealed a positive reaction in both groups. Higher expression of extracellular matrix (ICAM, Collagen IV) was observed in Group B. RT- PCR demonstrated a higher expression of inflammatory Cytokines in Group B.ConclusionWhile conventional cultivation method can be used for the development of tissue engineered heart valves. Better results can be obtained by performing a conditioning step that may improve the tolerance of cells to shear stress. The novel pulsatile bioreactor offers an adequate tool for in vitro improvement of mechanical properties of tissue engineered cardiovascular prostheses.

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Genetic Regulation of Endothelial Inflammatory Responses in Baboons

Cited by 14 publications

References 44 publications

Protective Effects of Resveratrol on TNF-α-Induced Endothelial Cytotoxicity in Baboon Femoral Arterial Endothelial Cells

Protective Effects of Resveratrol on TNF-α-Induced Endothelial Cytotoxicity in Baboon Femoral Arterial Endothelial Cells

Animal Models of Atherosclerosis

Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering

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