Genetic Relationship of Wickham and IRRDB 1981 Rubber Population Based on RAPD Markers Analysis

Oktavia, Fetrina; Kuswanhadi, Mudji Lasminingsih

doi:10.4308/hjb.18.1.27

Cited by 11 publications

(12 citation statements)

References 11 publications

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“…This results demonstrated that the newly introduced rubber tree accessions were differed and separated from cultivated rubber clones. These results are also similar to the genetic diversity estimates of the rubber tree using SSR markers reported by Lekawipat et al (2003) and those using RAPD (Oktavia & Kuswanhadi, 2011). The cluster analysis also showed that some accessions belonged to the sub-populations of PN'81 did not exactly reflect their geographical origins.…”

Section: Allelic and Population Genetic Diversitysupporting

confidence: 90%

“…Most of the population used in this study came from PN'81, the newly introduced rubber genetic materials, while some previous studies using mostly of Wickham associated clones (Lekawipat et al, 2003;Oktavia & Kuswanhadi, 2011). However, the estimated population parameters were lower than that observed by de .…”

Section: Allelic and Population Genetic Diversitymentioning

confidence: 70%

“…Wickham collected the stocks from one Brazilian site (Boim area), in the Western banks of the Tapajos River. Previous studies reported that those commercial rubber clones exhibited a very narrow genetic base (Besse et al, 1994;Lekawipat et al, 2003;Oktavia & Kuswanhadi, 2011) making current improvement of rubber clones difficult. Moreover, because of assortative mating, low fruit-set limitation, selection for the high latex yield character, and extensive use of particular clones as parents in rubber breeding programs, further reduce the genetic diversity of the commercial rubber germplasm (Priyadarshan, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity and Population Structure of IRRDB 1981 and Wickham Rubber Germplasm Based on EST-SSR

et al. 2017

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The accession of the IRDB 1981 (PN'81) population is a newly introduced and an important rubber tree germplasm while the Wickham clone is a commercial variety one. The objectives of this study were to assess the genetic diversity and the population structure of PN'81 populations and the Wickham clones using 15 EST-SSR loci. Results of the analysis showed that the evaluated SSR primers yielded polymorphic markers. The gSSR 268 primer pairs yielded the most informative markers while HBE 280 primers generated the lowest ones. Results of the genetic diversity analysis supported that the PN'81 population belonged to a single large natural population of rubber trees while the Wickham clones belonged to a different group than that of PN'81. The population structure analysis of the rubber accessions was also in agreement with the results of the genetic diversity analysis. The experiment also indicated that PN'81 populations would be useful for future rubber breeding in Indonesia, especially as the sources of parent clones for rubber tree hybridization programs and rubber tree genetic resource conservation.

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Section: Allelic and Population Genetic Diversitysupporting

confidence: 90%

Section: Allelic and Population Genetic Diversitymentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity and Population Structure of IRRDB 1981 and Wickham Rubber Germplasm Based on EST-SSR

et al. 2017

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“…Penelitian tersebut menyimpulkan bahwa koleksi plasma nutfah IRRDB 1981 di Vietnam memiliki karagaman genetik yang tinggi dan perbedaan interdistrict yang rendah. Oktavia et al, (2011a) telah menganalisis jarak genetik beberapa klon tanaman karet menggunakan marka RAPD. Amplifikasi DNA genom dari 45 klon menghasilkan 2.408 fragmen DNA yang berukuran 250-3.000 bp dengan kisaran matriks kesamaan genetik cukup luas (59,18%-94,23%).…”

Section: Marka Rapdunclassified

UPAYA PERBAIKAN GENETIK DAN PENYEDIAAN BIBIT TANAMAN KARET (Hevea brasiliensis Muell. Arg.) MELALUI PENDEKATAN BIOTEKNOLOGI

Darojat¹,

Tistama²

2014

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AbstrakBeberapa tantangan dalam kegiatan perakitan klon unggul baru tanaman karet (Hevea brasiliensis Muell. Arg.) melalui metode p e r s i l a n g a n b u a t a n ( k o nve n s i o n a l ) diantaranya adalah membutuhkan waktu yang lama, sifat heterozigositas yang tinggi dan tingkat keberhasilan yang belum optimal. Bioteknologi diharapkan dapat menjadi metode pendukung dalam menjawab t a n t a n g a n y a n g t e r j a d i t e r s e b u t . Perkembangan teknik in vitro dan rekayasa genetika telah memberikan terobosan baru dalam perbanyakan dan perbaikan genetik tanaman karet. Pemanfaatan bioteknologi telah berdampak pada proses perbaikan g e n e t i k t a n a m a n k a r e t d a l a m h a l produktivitas, kesehatan tanaman, dan peningkatan toleransi terhadap cekaman. Selain itu penggunaan marka molekuler sangat bermanfaat untuk percepatan seleksi dalam kegiatan pemuliaan tanaman karet. Teknik tersebut juga berguna dalam identifikasi klon dan tetua, analisis keanekaragaman genetik, pengawasan hasil persilangan buatan, penciri klon dan biologi reproduksi. Tulisan ini menjabarkan tentang pendekatan bioteknologi untuk meningkatkan potensi genetik tanaman karet.

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“…Several researchers have investigated the genetic diversity of rubber tree by using molecular markers (Lekawipat et al, 2003;Saha et al, 2005;Lam et al, 2009;Gouvêa et al, 2010;Oktavia et al, 2011), but there was no report about the polymorphism of lower repeats SSR markers and the loci and the flanking area variation of SSR markers in rubber tree. SSR marker used to detect the alleles by PAGE gel after PCR has slight restrictions in distinguishing the fragments as length or size homoplasy (Estoup et al, 1995;Grimaldi & Crouau-Roy, 1997;Angers & Bernatchez, 1997).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Genetic Diversity and SSR Allelic Variation in Rubber Tree (Hevea brasilensis)

Feng¹,

Wu²,

Li³

et al. 2012

The Molecular Basis of Plant Genetic Diversity

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Genetic Relationship of Wickham and IRRDB 1981 Rubber Population Based on RAPD Markers Analysis

Cited by 11 publications

References 11 publications

Genetic Diversity and Population Structure of IRRDB 1981 and Wickham Rubber Germplasm Based on EST-SSR

Genetic Diversity and Population Structure of IRRDB 1981 and Wickham Rubber Germplasm Based on EST-SSR

UPAYA PERBAIKAN GENETIK DAN PENYEDIAAN BIBIT TANAMAN KARET (Hevea brasiliensis Muell. Arg.) MELALUI PENDEKATAN BIOTEKNOLOGI

Analysis of Genetic Diversity and SSR Allelic Variation in Rubber Tree (Hevea brasilensis)

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