Genetic relationships among Stylosanthes species as revealed by sequence-tagged site markers

Stappen, J. Vander; Weltjens, I.; Campenhout, Steven Van; Volckaert, Guido

doi:10.1007/s001220051167

Cited by 16 publications

(18 citation statements)

References 19 publications

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“…Williams et al 1984;Stace and Cameron 1987;Vieira et al 1993). Recently, molecular marker techniques have proven to be very useful in providing additional data for delineating species (Liu et al 1999;Vander Stappen et al 1999b), assessing evolutionary relationships and elucidating the genetic origin of allopolyploids (Gillies and Abbott 1996;Vander Stappen et al 1999a;Liu and Musial 2001;Vander Stappen et al 2002a, b), and determining genetic diversity (Vander Stappen et al 1998Sawkins et al 2001) in the genus Stylosanthes. A large-scale phylogenetic analysis of Stylosanthes based on the internal transcribed spacer (ITS) region provided useful information about evolutionary relationships in the genus, especially regarding the allopolyploid species and the basic structure of the genus (Vander Stappen et al 2002a).…”

Section: Introductionmentioning

confidence: 98%

Molecular characterization and phylogenetic utility of the rDNA external transcribed spacer region in Stylosanthes (Fabaceae)

2003

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Supplementary Material The nucleotide sequence of the ribosomal external transcribed spacer (ETS) region of Stylosanthes mexicana was determined and used to evaluate its potential for examination of intra- and inter-specific relationships in Stylosanthes, as compared to the use of the internal transcribed spacer (ITS) region. The entire ETS region comprises 1,145 bp and is composed of a region of non-repetitive sequences consisting of three subregions with organizational and nucleotide-sequence conservation, and a triplicated segment of about 100 bp. A primer designed in the second conserved subregion allowed us to amplify and sequence directly the 3' part (423-431 bp) of the ETS from 22 genotypes of 12 representative Stylosanthes species that were previously used in phylogenetic analysis of the genus. The study revealed that the right-hand part of Stylosanthes ETS contains approximately twice as much variable and informative characters than the ITS. Moreover, pairwise sequence-divergence values are twice as high, on average, when compared to the ITS. The ITS and ETS datasets are consistent in phylogenetic reconstruction of Stylosanthes, and combined parsimony analysis resulted in a strict consensus tree that is better resolved and generally better supported than trees obtained from separate analysis of the spacer regions.

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Section: Introductionmentioning

confidence: 98%

Molecular characterization and phylogenetic utility of the rDNA external transcribed spacer region in Stylosanthes (Fabaceae)

2003

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“…This is more precisely required when the genetic stocks is employed for identification of QTLs for agronomical important traits (phenotypic data). Previous reports have indicated the use of STS in studying the genetic relationship and progenitor identification due to the additive nature of the marker (Vander Stappen et al 1999).…”

Section: Resultsmentioning

confidence: 99%

“…Fifteen STS primer pairs used in the present investigation were primarily derived from the Pst1 clone obtained from S. scabra cv Fitzroy and S. hamata cv Verano (Liu and Musial 1995) as well as different coding and non-coding regions of gene sequences (Curtis et al 1995, Smith et al 1995, Reddy et al 1996, Vander Stappen et al 1999. The OLIGO (version 3.0) computer program was used to select the optimal oligonucleotide for STS primer pairs as described by Liu et al (1996).…”

Section: Sts Methodologymentioning

confidence: 99%

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Molecular Assessment of Variation in Stylosanthes fruticosa Accessions Employing STS and RAPD Markers

Chandra

2007

CYTOLOGIA

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Summary Genetic variations in two accessions of S. fruticosa (41117 and 41117A) was carried out employing random amplified polymorphic DNA (RAPD) and sequence-tagged-sites (STS) markers to establish the accession closely resembles to the mapping population developed using one of these as a anthracnose susceptible parent along with S. scabra cv Seca as resistant source. Sixty-four RAPD and 15 STS primers generated 469 and 59 fragments respectively. Of these, 90 (19.2%) RAPD and 6 (10.2%) STS fragments showed polymorphism between two accessions of S. fruticosa. When same primers sets were used with F 2 progeny, the segregated RAPD fragments showed patterns belonging to S. scabra cv SecaϫS. fruticosa 41117A rather S. scabra cv SecaϫS. fruticosa 41117 cross. Of 15 STS primers pairs, SHPALF2/R2, SHST2F3/T2R16 and SHST1F3/T1R3 clearly demonstrated that 41117A line was closer to population as SHPALF2/R2 showed absence of a 480-bp fragment in S. fruticosa 41117 and present in other two parents i.e., S. scabra cv Seca and S. fruticosa 41117A and 78 F 2 plants tested whereas rest two primer pairs amplified a fragment of smaller size in 41117 only. Though most of the STS primer sequences used were derived from S. scabra cv Fitzroy and S. hamata cv Verano, the level of reaction in S. fruticosa accessions indicated low genome specificity of STS primers and hence low level of genetic divergence in different species of Stylosanthes. Results also provided the list of potential STS and RAPD primers could be used in studying the genetic relationships among S. fruticosa accessions, which is the only species of the genus Stylosanthes native and endemic to the southern peninsular regions of India, for plant conservation and breeding programs.

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“…This will further lead us to identify phenotypically contrast S. fruticosa accession could be used in developing mapping population to study the genetics of anthracnose resistance in Stylosanthes. Fifteen sequence-tagged-sites (STS) primers pairs (Manners et al 1995, Smith et al 1995, Liu et al 1996, Vander Stappen et al 1999 have been screened and in total 59 fragments have been amplified with the present parents would be further extended with population so that the polymorphic markers can be identified and placed to enrich the genetic map.…”

Section: Resultsmentioning

confidence: 99%

A Partial Genetic Map of Stylosanthes Developed Using Inter-Specific F2 Cross

Chandra

2006

CYTOLOGIA

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Summary Low polymorphism in Stylosanthes emphasizes use of inter-specific crosses for the development of mapping population for genetic linkage map. Using 78 F 2 individuals of the cross of S. scabra cv. Seca and S. fruticosa, 7 linkage groups were obtained when 81 polymorphic RAPD (Random Amplified Polymorphic DNA) markers were processed. Of these, 53 segregated in expected ratio of 3 : 1 for dominant marker in the F 2 generation, 11 markers in 1 : 1 while 17 deviated from the expected ratio. Thirty markers remain unlinked. Total genome length covered by markers in the present partial map is 630.4 cM (centi Morgan) with an average interval of 39.4 cM. The skeleton map developed is being used to place reasonable numbers of RAPD and STS (Sequence Tagged Sites) markers and to identify QTLs (Quantitative Trait Loci) for anthracnose disease, one of the most important disease of the genus Stylosanthes.

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Genetic relationships among Stylosanthes species as revealed by sequence-tagged site markers

Cited by 16 publications

References 19 publications

Molecular characterization and phylogenetic utility of the rDNA external transcribed spacer region in Stylosanthes (Fabaceae)

Molecular characterization and phylogenetic utility of the rDNA external transcribed spacer region in Stylosanthes (Fabaceae)

Molecular Assessment of Variation in Stylosanthes fruticosa Accessions Employing STS and RAPD Markers

A Partial Genetic Map of Stylosanthes Developed Using Inter-Specific F2 Cross

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