Genetic relationships between Lolium (Poaceae) species revealed by RAPD markers

Abstract

ABSTRACT. The genus Lolium is one of the most important groupings of temperate forage grasses, including about eight recognized species that are native to some temperate and subtropical regions of the northern hemisphere. We examined genetic relationships among 18 accessions representing all Lolium species using RAPD markers. Among 50 random primers that we screened, 13 gave reproducible amplification banding patterns. Each of these 13 primers generated 19-43 scorable fragments. A total of 367 RAPD fragments were detected, of which 95.9% were polymorphic across all the Lolium accessions. Dice's coefficient of dissimilarity ranged from 0.016 to 0.622, which is indicative of substantial genetic variations in these Lolium accessions. A neighbor-joining cluster analysis, with bootstrap permutation, produced an unrooted dendrogram, which grouped 18 accessions into two main clades, supporting high bootstrap values (98 and 96% The value of r = 0.97 in the Mantel test for cophenetic correlation applied to the cluster analysis indicated the high degree of fit of the accessions to a group. A principal coordinate analysis, whose first three coordinates explained 72.6% of the variation, showed similar groupings as in the cluster analysis. The genetic relationships estimated by the polymorphism of RAPD markers are basically in agreement with those previously inferred with other genetic markers.

SSRs transferability and genetic diversity of three allogamous ryegrass species

“…Also, analogous results have already been described via isozyme [40], morphological analysis, and electrophoresis of seed proteins [53,54]. Nevertheless, according to RAPD [55,56] or AFLP data [57], L. multiflorum has a position closer to that of L. perenne than to that of L. rigidum. Several factors might explain the above controversy, such as different clustering methods, characteristics of genetic markers, sample size, and number of studied species.…”

Genetic diversity and relationships in cultivars of Lolium multiflorum Lam. using sequence-related amplified polymorphism markers

“…However, there is little information about the genetic diversity of Lolium (Guthridge et al, 2001). Previous studies have used random amplification of polymorphic DNA (Ma et al, 2013), while SRAP was used to identify the parent generation.…”

Determination of Chromosome Structures of Genotypes of Perennial Ryegrass (<i>Lolium perenne</i> L.) Selected from Natural Vegetation of Turkey

“…There is no morphological difference between the annual L. rigidum and L. loliaceum, as they are similar in the asymmetric karyotype. According to the information from the scatter graph, it is thought that the genotypes in the groups 1 and 2 may be related to L. rigidum, L. multiflorum and L. perenne when the karyotype formula and chromosome asymmetry are considered (Ma et al 2013). In general, studies have shown that those with primitive type karyotypes are associated with L. rigidum and those with types of anthropometric symmetric chromosomes are associated with L. perenne or perennial L multiflorum.…”