Genetic relationships of Iranian Hypericum perforatum L. wild populations as evaluated by ISSR markers

Abstract

St. John's wort (Hypericum perforatum L.), as the most important medicinal species of the genus Hypericum, is medicinally used for many purposes such as treatment of mild and moderate depression. Considering importance of knowledge on genetic structure of wild relatives of crop plants, the present study was aimed to assess genetic variability among ten wild populations of H. perforatum growing in different climatic regions of Iran via ISSR markers. In general 15 selected primers generated 191 polymorphic fragments with an average of 12 in each primer. According to the UPGMA constructed dendrogram and PCoA, studied populations were classified into four main groups which was, to the some extent, in accordance with their geographical origins. Genetic distances of these populations ranged from 0.109 (Nor and Nowshahr) to 0.345 (Ardebil and Galogah). Also, Nei gene diversity (H e ) values were calculated to be from 0.066 to 0.216 and Shannon's information indices (I) varied from 0.321 to 0.097. Nei's genetic differentiation index (G st ) was high (0.573) indicating the high levels of genetic variation among the studied populations. Finally, the gene flow (N m ) value was found to be 0.372. Obtained information is useful for use of Iranian H. perforatum germplasm for breeding programs and for development of effective conservation strategies.

Factors affecting the variation of bioactive compounds inHypericumspecies

“…Haluskova and Cellarova (1997) analyzed the somaclonal variation within H. perforatum and R1 progenies at the molecular level via restriction fragment length polymorphism (RFLP) technique, whereas Percifield et al (2007) practiced DNA fingerprinting using amplified fragment length polymorphism (AFLP) technique to search genetic diversity among 11 Hypericum species. Farooq et al (2014) from India and Morshedloo et al (2015) from Iran assessed the genetic diversity of H. perforatum wild populations from different climatic zones using intersimple sequence repeat markers. Sixteen Tunisian populations of H. humifusum L. were evaluated for their genetic variability using isomeric and random-amplified polymorphic DNA (RAPD) markers (Bejaoui et al, 2010(Bejaoui et al, , 2012.…”

Chemical characterization of the essential oil compositions from Iranian populations of Hypericum perforatum L.

“…Cluster analysis (CA) was used to characterize the samples according to the geographical origin. The presented work is part of our ongoing research programs on H. perforatum, aimed at detecting interesting genotypes for industrial use and conservation management (Morshedloo et al, 2015).…”

Genetic diversity and relationships among Hypericum L. species by ISSR Markers: A high value medicinal plant from Northern of Iran

“…However, defining the criterion which could address the boundaries of species is different and the place of debates (Esfandani- Bozchaloyi et al 2018aBozchaloyi et al , 2018bBozchaloyi et al , 2018cBozchaloyi et al , 2018d. Wild relatives of crops contain genes with the great potential for use in breeding programs and constitute a part of their gene pool (Pandey et al 2008). In addition, the study of intra-specific levels of genetic variation and investigation of genetic structure of wild populations is crucial for development of effective conservation strategies.…”