Genetic Reprogramming of Positional Memory in a Regenerating Appendage

Wang, Ying-Ting; Tseng, Tzu-Lun; Kuo, Yu-Chia; Yu, Jr-Kai; Su, Yi-Hsien; Poss, Kenneth D.; Chen, Chen‐Hui

doi:10.1016/j.cub.2019.10.038

Cited by 25 publications

(23 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wound sites and the neighboring tissues act as signaling centers in many regenerating organisms, instructing the re-patterning of body parts or blastema initiation – for example in Hydra polyps ( Chera et al, 2009 ), zebrafish caudal fins ( Wang et al, 2019 ), or planarians ( Oderberg et al, 2017 ; Petersen and Reddien, 2009 ; Reddien and Sánchez Alvarado, 2004 ; Wurtzel et al, 2015 ). We found an analogous correlation between wound site and blastema position, however in Clytia medusa signaling from the wound site is not sufficient to trigger regeneration, but it is likely conditional.…”

Section: Discussionmentioning

confidence: 99%

Pattern regulation in a regenerating jellyfish

Sinigaglia

Péron

Eichelbrenner

et al. 2020

eLife

View full text Add to dashboard Cite

Clytia hemisphaerica jellyfish, with their tetraradial symmetry, offer a novel paradigm for addressing patterning mechanisms during regeneration. Here we show that an interplay between mechanical forces, cell migration and proliferation allows jellyfish fragments to regain shape and functionality rapidly, notably by efficient restoration of the central feeding organ (manubrium). Fragmentation first triggers actomyosin-powered remodeling that restores body umbrella shape, causing radial smooth muscle fibers to converge around 'hubs' which serve as positional landmarks. Stabilization of these hubs, and associated expression of Wnt6, depends on the configuration of the adjoining muscle fiber 'spokes'. Stabilized hubs presage the site of the manubrium blastema, whose growth is Wnt/β-catenin dependent and fueled by both cell proliferation and long-range cell recruitment. Manubrium morphogenesis is modulated by its connections with the gastrovascular canal system. We conclude that body patterning in regenerating jellyfish emerges mainly from local interactions, triggered and directed by the remodeling process.

show abstract

Section: Discussionmentioning

confidence: 99%

Pattern regulation in a regenerating jellyfish

Sinigaglia

Péron

Eichelbrenner

et al. 2020

eLife

View full text Add to dashboard Cite

show abstract

“…Treatment with hydroxyurea, an inhibitor of DNA replication, in the early stages of regeneration (2-5 dpa) results in smaller tails, further supporting a role for cell proliferation (Wang et al, 2019). The source is still unknown, but may include a population of resident stem cells associated with myofibres, termed muscle satellite-like stem cells, dedifferentiated muscle fibers generated as the myosepta near the amputation plane degenerate (Somorjai et al, 2012b), or even coelomocytes.…”

Section: Cephalochordatamentioning

confidence: 98%

Beyond Adult Stem Cells: Dedifferentiation as a Unifying Mechanism Underlying Regeneration in Invertebrate Deuterostomes

Ferrario

Sugni

Somorjai

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

The diversity of regenerative phenomena seen in adult metazoans, as well as their underlying mechanistic bases, are still far from being comprehensively understood. Reviewing both ultrastructural and molecular data, the present work aims to showcase the increasing relevance of invertebrate deuterostomes, i.e., echinoderms, hemichordates, cephalochordates and tunicates, as invaluable models to study cellular aspects of adult regeneration. Our comparative approach suggests a fundamental contribution of local dedifferentiation -rather than mobilization of resident undifferentiated stem cells-as an important cellular mechanism contributing to regeneration in these groups. Thus, elucidating the cellular origins, recruitment and fate of cells, as well as the molecular signals underpinning tissue regrowth in regenerationcompetent deuterostomes, will provide the foundation for future research in tackling the relatively limited regenerative abilities of vertebrates, with clear applications in regenerative medicine.

show abstract

“…Treatment with TH throughout regeneration is sufficient to robustly rescue distal identity (Figure 3B). Previous studies have shown that cellular memory within the blastema dictates fin size during regeneration (Tornini et al, 2016; Wang et al, 2019), and we asked if the TH rescue of distal morphology was due to effects of the hormone on the blastema. We treated hypothyroid fish with TH (in the form of T4) for 24 hours during each of the first 3 days post amputation (dpa), but found that none of these treatments were sufficient to rescue distal patterning in regenerates (Figure 3—Figure Supplement 1).…”

Section: Resultsmentioning

confidence: 99%

Thyroid hormone regulates proximodistal identity in the fin skeleton

Harper

Acosta

et al. 2020

Preprint

View full text Add to dashboard Cite

Across the ~30,000 species of ray-finned fish, fins show incredible diversity in overall shape and in the patterning of the supportive bony rays. Fin length mutant zebrafish have provided critical insights into the developmental pathways that regulate relative fin size. However, the processes that govern skeletal patterning along the proximodistal axis of the fin have remained less well understood. Here, we show that thyroid hormone (TH) regulates proximodistal identity of fin rays in terms of pattering, gene expression profiles, and morphogenetic processes during outgrowth. This role for TH in specifying proximodistal identity appears conserved between development and regeneration, in all the fins, and between species. We demonstrate that proximodistal identity is regulated independently from pathways that determine size, and we show that modulating proximodistal patterning relative to growth can recapitulate components of the vast fin ray diversity found in nature.

show abstract

Genetic Reprogramming of Positional Memory in a Regenerating Appendage

Cited by 25 publications

References 56 publications

Pattern regulation in a regenerating jellyfish

Pattern regulation in a regenerating jellyfish

Beyond Adult Stem Cells: Dedifferentiation as a Unifying Mechanism Underlying Regeneration in Invertebrate Deuterostomes

Thyroid hormone regulates proximodistal identity in the fin skeleton

Contact Info

Product

Resources

About