To determine the natural variation for sucrose and raffinose fannily oligosaccharides (RFO) concentration in lentils {Lens culinaris Medik. ssp. culinaris), we analyzed a subset of 168 Lens germplasm lines from the ICARDA collection. Significant (P < 0.05) variations were found for sucrose, total RFO concentrations, and composition of the three main RFO (specifically raffinose, stachyose, and verbascose)