Genetic similarity between staphylococcus sp isolated from human and hospital settings, and susceptibility to different antimicrobials

This study was undertaken to determine the prevalence of coagulase positive staphylococcus (CPS) in Jordan and to investigate the presence of genes encoding exfoliative toxins (eta, etb), and toxic shock syndrome toxin-1 (tst). Seven hundred and fifty three samples were used including 273 obtained from human sources and 480 from animals (sheep, cows, and goats). One hundred and sixty seven isolates were identified as CPS and appeared as gram positive cocci, non-motile, produced coagulase, catalase, reduce tellurite, were resistant to acriflavin, unable to produce oxidase and amylase. The prevalence of CPS colonizing human was 115(42.1%) with 26.0% in nasal and 16.0% in nails. Livestock-associated CPS was detected in 52(10.8%) of the samples. polymerase chain reaction (PCR) amplification revealed eta to be the most common toxin gene detected in 36.5 and 28.8%, followed by tst in 25.2 and 5.76% of human and animal isolates, respectively. The possession of various gene combinations was found in 15(8.98%) of the isolates including eta plus tst in 14(12.2%) and eta plus etb in 1(0.86%) of human isolates. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was performed for all CPS by using TaqI restriction; the pattern revealed that 163(97.6%) were Staphylococcus aureus and were identified phenotypically and confirmed genotypically by amplification of kat gene, and 4(2.40%) identified as Staphylococcus pseudintermedius. Phylogenetic analysis indicated that clones characterized in this study were S. aureus subsp. aureus and S. pseudintermedius. Toxins genes are mostly prevalent among S. aureus subsp. aureus strains.

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“…E-mail: hala2002dagh@yahoo.com. (Fabiano et al, 2008) and were kept at 4°C for identification (Abdul Aziz et al, 2013).…”

Section: Human Sourcesmentioning

confidence: 99%

Prevalence of exfoliative toxins and toxic shock syndrome toxin-1 encoding genes among coagulase positive Staphylococcus isolated from human and animal sources

Al-Daghistani¹,

Hassawi²,

Hasan³

et al. 2016

Afr. J. Microbiol. Res.

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“…Phenotypic and genotypic characteristics of S. aureus aureus in the nasopharynx, 30% are intermittent carriers, and 50% are non-carriers (15,45).…”

Section: Giarola Lb Et Almentioning

confidence: 99%

Carriage frequency, phenotypic and genotypic characteristics of Staphylococcus aureus isolated from dialysis and kidney tranplant patients at a hosptial in northern Paraná

Giarola¹,

Santos²,

Tognim³

et al. 2012

Braz. J. Microbiol.

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The objective of the present study was to determine the frequency of Staphylococcus aureus nasal carriage among dialysis and kidney transplant patients, to identify the antimicrobial resistance profile of these strains and to verify their genetic profiles with the RW3A primer. The study included 159 individuals, comprising 111 dialysis and 48 kidney transplant patients. Of the 48 transplant patients, 75% were positive for S.aureus, whereas 49% of the 111 dialysis patients were carriers. Two samples yielded conflicting results for oxacillin sensitivity between the disk diffusion and minimum inhibitory concentration (MIC) assays: both were sensitive by the disk diffusion assay and resistant by MIC (4 μg/ml). In the antibiogram by disk diffusion, ten samples were resistant to cefoxitin, among which eight were also resistant to oxacillin. The resistance of the ten samples to cefoxitin by the disk diffusion assay was confirmed by MIC. Of the ten oxacillin-resistant samples, eight harbored the mecA gene. All samples were sensitive to vancomycin, and most were resistant to penicillin and demonstrated high rates of resistance to the other antimicrobials tested.The samples from dialysis patients exhibited a more homogenous genetic profile. Among the samples with a high percent similarity, no correlation with sensitivity or resistance to oxacillin was observed. According to the results of this study, the implementation of prevention and control measures, such as increased restrictions on prescriptions for antimicrobial drugs and nasal decontamination prior to high-risk procedures, is recommended.

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“…S. aureus primarily residues on the mucous membranes of the human nasopharynx, as well as on human and animal skin (15,29).…”

Section: Introductionmentioning

confidence: 99%

The hydrophobicity and roughness of a nasoenteral tube surface influences the adhesion of a multi-drug resistant strain of Staphylococcus Aureus

Lima¹,

Andrade²,

Soares³

et al. 2011

Braz. J. Microbiol.

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In this study, we examined the physiochemical properties of nasoenteral feeding tubes made from two different types of polymer: silicone materials and polyurethane. The internal surfaces of the nasoenteral feeding tubes were analyzed for their hydrophobicity, roughness, microtopography, rupture-tension and ability to stretch. We also studied the adhesion of an isolated, multi-drug resistant strain of S. aureus to these polymers. The polyurethane nasoenteral tube, which was classified as hydrophilic, was more resistant to rupture-tension and stretching tests than the silicone tube, which was classified as hydrophobic.Additionally, the polyurethane tube had a rougher surface than the silicone tube. Approximately 1.0 log CFU.cm -2 of S. aureus cells adhered to the tubes and this number was not statistically different between the two types of surfaces (p > 0.05). In future studies, new polymers for nasoenteral feeding tubes should be tested for their ability to support bacterial growth. Bacterial adhesion to these polymers can easily be reduced through modification of the polymer's physicochemical surface characteristics.

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Genetic similarity between staphylococcus sp isolated from human and hospital settings, and susceptibility to different antimicrobials

Cited by 4 publications

References 24 publications

Prevalence of exfoliative toxins and toxic shock syndrome toxin-1 encoding genes among coagulase positive Staphylococcus isolated from human and animal sources

Prevalence of exfoliative toxins and toxic shock syndrome toxin-1 encoding genes among coagulase positive Staphylococcus isolated from human and animal sources

Carriage frequency, phenotypic and genotypic characteristics of Staphylococcus aureus isolated from dialysis and kidney tranplant patients at a hosptial in northern Paraná

The hydrophobicity and roughness of a nasoenteral tube surface influences the adhesion of a multi-drug resistant strain of Staphylococcus Aureus

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