Genetic stability of long-term micropropagated Opuntia ficus-indica (L.) Mill. plantlets as assessed by molecular tools: Perspectives for in vitro conservation

Zoghlami, Nèjia; Bouamama, Badra; Khammassi, Manel; Ghorbel, Abdelwahed

doi:10.1016/j.indcrop.2011.08.006

Cited by 25 publications

(11 citation statements)

References 15 publications

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“…Genetic stability among Prickly pear cultivars (Opuntia cus-indica L.) was also studied using RAPD markers. Out of 87 amplicons, only 82 bands were monomorphic produced, and overall 91 % of genetic similarity among the plantlets (Zoghlami et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…PCR based single primer ampli cation reaction (SPAR) techniques using microsatellites, simple sequence repeats (SSR), inter simple sequence repeats (ISSR), random ampli ed polymorphic DNA (RAPD), and directed ampli cation of minisatellite DNA (DAMD) primers to analyse and detect the genetic stability among the in vitro generated plants are the most common practices in plant tissue culture experiments. Techniques which utilise single primers to amplify the DNA in PCR reactions are generally applicable universally (Zoghlami et al 2012;Lamare and Rao, 2015;Baghel and Bansal, 2017;Rohela et al 2019). The use of ISSR and DAMD markers has proven to be unbiased and cost-effective as compared with the RAPD markers (Singh et al 2014;Purayil et al 2018;Meena et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of genetic stability on in vitro and ex vitro plants of Ficus carica var. black jack using ISSR and DAMD markers

2021

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In vitro propagation has been signi cant in producing a large number of genetically stable regenerated plants. Regenerated Ficus carica var. Black Jack plantlets were established using woody plant medium (WPM) supplemented with 20 µM 6-Benzylaminopurine (BAP) and 8 µM Indole-3-acetic acid (IAA) under different light treatments such as normal uorescent white light (60 µmol.m − 2 .s − 1 ), and four different LED spectra, white (400-700nm), blue (440nm), red (660nm) and blue + red (440nm + 660nm). Genetic stability analysis was performed on the in vitro and ex vitro plants of Ficus carica var. Black Jack. Ten (10) primers of each ISSR and DAMD molecular marker were used to assess the genetic stability of the eight (8) samples of Ficus carica var. Black Jack, acquired over two years. The ndings of this study revealed that inter simple sequence repeats (ISSR) and directed ampli cation of minisatellite DNA (DAMD) markers (DNA primers) are e cient in determining the polymorphism and monomorphism percentage among the in vitro and ex vitro samples of Ficus carica var. Black Jack. ISSR markers showed 97.87% of monomorphism whereas DAMD markers showed 100% monomorphism. Polymorphism of 2.13% was observed for the UBC840 ISSR -DNA primer which was negated under the genetic similarity index analysis for the eight samples. It is recommended that genetic stability analysis should be performed for long-term maintenance of micropropagated plants.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of genetic stability on in vitro and ex vitro plants of Ficus carica var. black jack using ISSR and DAMD markers

2021

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“…Indeed, single cuttings can be one-to two-years-old, and their surface area, dry mass and environmental factors, such as soil fertility and water availability, have a significant influence on successful rooting and subsequent performance in the field (Inglese et al, 2002). The hypothesis that the observed variability is the result of somaclonal mutation does not seem very likely, as shown by Zoghlami et al (2012), who observed the genetic stability of long-term micropropagated OFI plantlets. However, we should not to exclude that in some ecotypes, the intra-population variability might be due to the existence of polyclonality.…”

Section: Biomass Production Assessmentmentioning

confidence: 99%

ABOVE-GROUND BIOMASS ESTIMATION OF OPUNTIA FICUS-INDICA (L.) MILL. FOR FORAGE CROP IN A MEDITERRANEAN ENVIRONMENT BY USING NON-DESTRUCTIVE METHODS

et al. 2016

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SUMMARYIn marginal lands Opuntia ficus-indica (OFI) could be used as an alternative fruit and forage crop. The plant vigour and the biomass production were evaluated in Portuguese germplasm (15 individuals from 16 ecotypes) by non-destructive methods, 2 years following planting in a marginal soil and dryland conditions. Two Italian cultivars (Gialla and Bianca) were included in the study for comparison purposes. The biomass production and the plant vigour were estimated by measuring the cladodes number and area, and the fresh (FW) and dry weight (DW) per plant. We selected linear models by using the biometric data from 60 cladodes to predict the cladode area, the FW and the DW per plant. Among ecotypes, significant differences were found in the studied biomass-related parameters and several homogeneous groups were established. Four Portuguese ecotypes had higher biomass production than the others, 3.20 Mg ha−1 on average, a value not significantly different to the improved ‘Gialla’ cultivar, which averaged 3.87 Mg ha−1. Those ecotypes could be used to start a breeding program and to deploy material for animal feeding and fruit production.

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“…Opuntia crop could be established in drought regions of many countries, including Africa and India (Ennouri et al 2006). Germplasm conservation, genetic engineering and tissue culture are key strategies in successful implementation of this vision (Shedbalkar et al 2010;Zoghlami et al (2012). Axillary-branching micropropagation technique for high degree of clonal fidelity and conservation of interesting genetic resources was recommended.…”

Section: Strategies To Reduce Postharvest Loss and Rolementioning

confidence: 99%

Opuntia Fruits as a Source of Inexpensive Functional Food

Patel

2015

Emerging Bioresources With Nutraceutical and Pharmaceutical Prospects

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Opuntia (prickly pear) is a genus of xerophyte belonging to the Cactaceae family, growing abundantly in the arid parts of the world. Owing to high nutritional value in terms of dietary fiber, polyunsaturated fatty acid, minerals, protein and an assortment of other phytochemicals, Opuntia fruits are gaining popularity. Validation of health benefits viz. antioxidant, anti-inflammatory, antidiuretic, hypocholesterolemic, antidiabetic, antiproliferation, immunostimulatory and antiulcerogenic activity have further consolidated their position in emerging nutraceutical sector. Though at infancy, the Opuntia fruit-based foods are garnering consumer approval and are expected to contribute significantly to functional food processing. This chapter explores the proven biological potentials and suggests future scopes.

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Genetic stability of long-term micropropagated Opuntia ficus-indica (L.) Mill. plantlets as assessed by molecular tools: Perspectives for in vitro conservation

Cited by 25 publications

References 15 publications

Assessment of genetic stability on in vitro and ex vitro plants of Ficus carica var. black jack using ISSR and DAMD markers

Assessment of genetic stability on in vitro and ex vitro plants of Ficus carica var. black jack using ISSR and DAMD markers

ABOVE-GROUND BIOMASS ESTIMATION OF OPUNTIA FICUS-INDICA (L.) MILL. FOR FORAGE CROP IN A MEDITERRANEAN ENVIRONMENT BY USING NON-DESTRUCTIVE METHODS

Opuntia Fruits as a Source of Inexpensive Functional Food

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