Genetic structure and molecular variability of potato virus M populations

Abstract

To investigate the genetic diversity of potato virus M (PVM; genus Carlavirus, family Betaflexiviridae), the complete nucleotide sequence of the coat protein gene of 30 PVM isolates from a major potato-growing region in Iran were determined. Phylogenetic analysis of these Iranian PVM isolates together with those available in the GenBank database suggested two divergent evolutionary lineages that did not reflect the origin of the isolates, and these were designated as PVM-o and PVM-d. Examination of the genetic variability of the coat protein of Iranian isolates and their counterparts whose sequences are available in the Genbank database revealed 16 genotype groups in the PVM population. Analysis of the synonymous-tononsynonymous ratio showed strong purifying selection in the CP gene in the genotype groups of divergent clades.

Molecular variability in the cysteine rich protein of potato virus M

“…The lack of association of isolate variation with geographic location highlights the world-wide movement of potato germplasm. Similar results have been reported for PVS [2,16], PVM [18,21], PVP [6] and Hop mosaic virus (HpMV) [15].…”

“…Previously, we showed different isolates of PVM have been segregated into two divergent evolutionary lineages of PVM-o (PVMordinary) and PVM-d (PVM-divergent) based on analysis of the CP gene, [18]. Surprisingly, this differentiation was also largely confirmed by analysis of the PVM CRP gene.…”

“…Previously, we showed that all PVM isolates evolve in two distinct divergent groups based on analysis of their coat protein gene [18]. The objective of this work is to investigate the diversity of the CRP protein in the PVM population and to study force acting on evolution of this gene in the PVM population.…”

Cross‐protection between a Naturally Occurring Mild Isolate of Potato virus M (PVM) and a More Virulent Isolate in Datura metel Plants

“…; Tabasinejad et al. ), the majority of the isolates being arranged within the two clades irrespective of their country of origin (Tabasinejad et al. ).…”

“…PVM isolate named PVM-ID has been reported to greatly differ from the ordinary virus strain in detectability by antisera raised against type PVM antigen (Cavileer et al 1998), which may imply the existence of different PVM serotypes. Recently, the phylogenetic trees revealed the segregation of PVM isolates originating in different countries into two main divergent evolutionary lineages (Xu et al 2010;Tabasinejad et al 2014), the majority of the isolates being arranged within the two clades irrespective of their country of origin (Tabasinejad et al 2014).…”