Genetic structure identification and assessment of interrelationships between Brassica and allied genera using newly developed genic-SSRs of Indian Mustard ( Brassica juncea L.)

Singh, B. K.; Choudhary, Shashi Bhushan; Yadav, Sushma; Malhotra, Era Vaidya; Rani, Reema; Ambawat, Supriya; Priyamedha, .; Pandey, Avinash; Kumar, Rishikesh; Kumar, Sudhir; Sharma, Hariom Kumar; Singh, Devendra Kumar; Rai, P.

doi:10.1016/j.indcrop.2018.01.023

Cited by 17 publications

(6 citation statements)

References 44 publications

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“…Our results for ranges between 5.36 (UBC834 -UBC845) and 14.57 (UBC827). The mean value for overall markers was 8.29, which was really high with respect to previous studies (Gupta et al, 2014;Kalita et al, 2007;Singh et al, 2018;Teklewold & Becker, 2006;Thakur et al, 2017a), at which different species of Brassica genus were investigated.…”

Section: Discussionsupporting

confidence: 45%

“…There is a lot of research investigating the relationships in the Brassicaceae family by using microsatellite markers (El-Esawi et al, 2016;Singh et al, 2018;Thakur et al, 2017b). Also, there are a lot of studies in which specifically ISSR markers used to determine genetic relationships in the Brassicaceae family (Khalil & El-Zayat, 2019;Safari et al, 2013;Shen et al, 2016;Wang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

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Genetic diversity analysis of some species in Brassicaceae family with ISSR markers

Maraş-Vanlioğlu¹,

Yaman²,

Kayaçetin³

2020

biost

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Brassicaceae is one of the biggest family which have thousands of species all around the world. In order to use wild mustard in a breeding process, their genetic kinship levels must be defined. Inter simple sequence repeats (ISSRs) are one of the common markers to evaluate genetic diversity. Here, 28 mustard genotypes representing four taxa, 17 of Brassica juncea, 2 of B. nigra, 2 of B. rapa, and 7 of B. arvensis, were investigated with seven ISSR primers. Totally, 160 bands were scored out of which 88.75% showed polymorphism. The polymorphism information content (PIC) varied from 0.25 to 0.40. The average heterozygosity (), multiplex ratio (), marker index (), and resolving power () were calculated as 0.33, 9.07, 2.99, and 8.29, respectively. STRUCTURE (v. 2.3.4) analysis unraveled two subpopulations (K=2). The dendrogram, constructed based on Jaccard similarity coefficient using the Unweighted Pair Group Average (UPGMA), in which, the first branch consisted of B. juncea, B. nigra and B. rapa, and the second branch consisted of B. arvensis, supported the results of STRUCTURE analysis. Additionally, principal component analysis (PCA) analysis supported the dendrogram and clearly separated the four taxa. This study showed that ISSRs would be useful to determine the genetic diversity in the Brassicaceae family.

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Section: Discussionsupporting

confidence: 45%

Section: Discussionmentioning

confidence: 99%

Genetic diversity analysis of some species in Brassicaceae family with ISSR markers

Maraş-Vanlioğlu¹,

Yaman²,

Kayaçetin³

2020

biost

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show abstract

“…There are many researches examining the relationships in the Brassicaceae family by using microsatellite markers (El-Esawi et al, 2016;Singh et al, 2018;Thakur et al, 2017). In addition, there are many studies in which specifically ISSR markers used to determine the genetic relationship in the Brassicaceae family (Khalil & El-Zayat, 2019;Safari et al, 2013;Shen et al, 2016;Wang et al, 2017).…”

Section: -+ ---+ + -mentioning

confidence: 99%

Histological Studies of some Brassicaceous Samples and the Genetic Variability Analysis using SCOT Markers

El-Shaht

El-Hefnawy

Hamza

et al. 2022

Journal of Agricultural Chemistry and Biotechnology

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Brassicaceae family (Cruciferae) known as mustard family, it is one of the largest families of flowering plants which have 372 genera and 4060 species all around the world. This study aims to investigate comparatively the anatomical structure of some plant samples of this family. Anatomical sections were taken on both stems and leaves, and 42 different qualitative characters were recorded in a data matrix. The results were numerically analysis to know the similarity between these samples by draw a dendrogram. Then the anatomical results were used to determine the genetics relationships between studied cultivated genera and the wild genera in order to includ in the breeding and improvement programs. Scot methods was chosen as it is fast, effective and gives accurate results. Start codon targeted (SCoT) polymorphism are one of the common markers were used to assess genetic diversity in some genera in Brassicaceae family. Seventin genera of the Brassicaceae have been used with ten SCoT primers. Totally, 174 bands were scored out of which 84.5% showed polymorphism, where 149 polymorphic bands and 25 monomorphic bands were observed. The results indicated that the existence of a significant degree of genetic similarity ranged from 0.62 to 0.88, where the highest mean value was 0.88 between genera 12 (Morettia canscens) and 13 (Moricandia siniaca) and the lowest mean value was 0.62 between genera 2 (Brassica napus) and 15 (Sinapis alba).

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“…Indian mustard [ Brassica juncea (L.) Czern & Coss.] is the most widely cultivated oilseed crop in India, out of six economically important species of the rapeseed–mustard group, due to its greater sustainability to grow under diverse agro-climatic conditions [ 5 , 6 , 7 ]. Natural amphiploid (2n = 4x = 36, AABB) India mustard is developed by natural crossing and genome doubling between two diploid progenitors, Brassica campestris (2n = 2x = 20, AA) and Brassica nigra (2n = 2x = 16, BB) [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis for Resistance to Sclerotinia Stem Rot, Yield and Its Component Traits in Indian Mustard [Brassica juncea (L.) Czern & Coss.]

Singh

Avtar

Kumar

et al. 2022

Plants

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Understanding the mode of gene action that controls seed yield and Sclerotinia stem rot resistance in Indian mustard is critical for boosting yield potential. In a line × tester mating design, ten susceptible lines and four resistant testers were used to conduct genetic analysis. The significance of general combining ability (GCA) and specific combining ability (SCA) variances revealed that both additive and non-additive gene actions were involved in the inheritance of Sclerotinia stem rot resistance and yield attributing traits. In addition to 1000-seed weight and number of primary and secondary branches/plant, the genotypes RH 1569 (line) and DRMR 2035 (tester) appeared to be the strongest general combiners for Sclerotinia stem rot resistance. RH 1657 × EC 597317 was the only cross among several that demonstrated a significant desired SCA value for Sclerotinia rot resistance. Regarding SCA effects for yield and component traits, the cross RH 1658 × EC 597328 performed best, with a non-significant but acceptable negative SCA effect for resistance. DRMR 2035, RH 1222-28, RH 1569, RH 1599-41, RH 1657, RH 1658, and EC 597328 are promising genotypes to use as parents in future heterosis breeding and for obtaining populations with high yield potential and greater resistance to Sclerotinia stem rot disease in Indian mustard, based on GCA effects of parents, per se performance, and SCA effects of hybrids. Days to 50% flowering, number of primary branches/plant, main shoot length, and 1000-seed weight all had a high genotypic coefficient of variability (GCV), broad-sense heritability (h2bs), and genetic advance as percent of the mean (GAM) values, as well as significant and desirable correlations and direct effects on seed yield. As a result, these traits have been recognized as the most critical selection criterion for Indian mustard breeding programs.

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Genetic structure identification and assessment of interrelationships between Brassica and allied genera using newly developed genic-SSRs of Indian Mustard ( Brassica juncea L.)

Cited by 17 publications

References 44 publications

Genetic diversity analysis of some species in Brassicaceae family with ISSR markers

Genetic diversity analysis of some species in Brassicaceae family with ISSR markers

Histological Studies of some Brassicaceous Samples and the Genetic Variability Analysis using SCOT Markers

Genetic Analysis for Resistance to Sclerotinia Stem Rot, Yield and Its Component Traits in Indian Mustard [Brassica juncea (L.) Czern & Coss.]

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