Genetic structure in peripheral Western European populations of the endangered species Cochlearia pyrenaica (Brassicaceae)

Cires, Eduardo; Samain, Marie‐Stéphanie; Goetghebeur, Paul; Prieto, José Antonio Fernández

doi:10.1007/s00606-011-0500-9

Cited by 16 publications

(15 citation statements)

References 44 publications

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“…(Nicoletti et al ., ) and Cochlearia pyrenaica DC. (Cires et al ., ). High levels of within‐population diversity may point to high outcrossing rates in dog roses, as detected by Kellner et al .…”

Section: Discussionmentioning

confidence: 97%

“…(Nicoletti et al, 2012) and Cochlearia pyrenaica DC. (Cires et al, 2011). High levels of within-population diversity may point to high outcrossing rates in dog roses, as detected by Kellner et al (2012), who found a high percentage of spontaneous hybrids (45%) between the tetraploid R. mollis and the diploid R. rugosa in the field.…”

Section: Genetic Diversity Within Populationsmentioning

confidence: 94%

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Low genetic and morphological differentiation in the European species complex ofRosa sherardii,R. mollisandR. villosa(RosasectionCaninaesubsectionVestitae)

2013

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The polyploid species complex of Rosa villosa sensu lato (Rosa section Caninae subsection Vestitae) consists of three morphologically similar polyploid species: R. sherardii, R. mollis and R. villosa. Whereas R. sherardii is distributed in central Europe, R. mollis and R. villosa represent a vicariant species pair occurring in northern Europe and in mountains of central and eastern Europe, respectively. In this study we analysed multiple data sets (morphology, cytology, microsatellites, AFLP and plastid DNA sequences) to re‐evaluate the systematics of these species and to examine whether cytological differences are reflected in the taxonomy and geographical distributions. Furthermore, these data sets were used to evaluate hypotheses explaining the vicariant distribution of R. mollis and R. villosa. None of these data sets revealed a clear‐cut differentiation between the species. Cytological and molecular data argued for a discrete taxonomic position of the predominantly pentaploid R. sherardii, but these data did not support a separation between the mostly tetraploid R. mollis and R. villosa. Population genetics revealed that samples of the latter species were assembled according to ploidy, but not to species affiliation or geographical distribution. Thus, we assume that the cytologically polymorphic original species had a continuous range prior to the last glaciation period and survived on nunataks or in non‐glaciated coastal regions in northern Europe, but that it failed to recover its former range after the retreat of the ice sheets. © 2013 The Linnean Society of London, Botanical Journal of the Linnean Society, 2014, 174, 240–256.

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Section: Discussionmentioning

confidence: 97%

Section: Genetic Diversity Within Populationsmentioning

confidence: 94%

Low genetic and morphological differentiation in the European species complex ofRosa sherardii,R. mollisandR. villosa(RosasectionCaninaesubsectionVestitae)

2013

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“…In a second selective amplification, the number of fragments was further reduced by adding primers with a three base pair extension: 9 primer combinations (Applied Biosystems) were used (Table I), which already revealed high resolution in a previous study of the genus Ranunculus (Cires 2011). For a more detailed explanation of the AFLP technique and quality parameters employed, see Cires et al (2011). Selective amplification products were sent to the University of Oviedo Sequencing Services for fragment analysis: samples were run on an automated DNA sequencer (ABI PRISM 1 3100, Applied Biosystems) with an internal size standard GeneScan 500 (ROX TM , Applied Biosystems).…”

Section: Aflp Analysismentioning

confidence: 99%

Ranunculus×prieti(Ranunculaceae): A new hybrid from the Cantabrian Mountains (North of Spain) revealed by morphological and molecular analyses

Cires

2012

Plant Biosystems - An International Journal Dealing with all As

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A new nothospecies Ranunculus 6prieti is described following morphological and molecular characterization. Hypervariable Amplified Fragment Length Polymorphism (AFLP) markers, flow cytometry (FCM) analysis and pollen viability were conducted. The profiles of hybrid samples were compared to their presumed parental taxa R. amplexicaulis and R. gramineus.Morphological study revealed that all hybrid individuals were morphologically intermediate between their parents, showing ovate-lanceolate basal leaves and amplexicaul cauline leaves as in R. amplexicaulis and yellow corollas as in R. gramineus. Principal coordinates analysis of the AFLP data set showed that hybrids are closer to R. gramineus than to R. amplexicaulis, whereas the results obtained by flow cytometry indicate otherwise. Pollen fertility estimation of R. 6prieti ranged from 68.83 to 95.81%, showing that hybrid samples are well established and fertile.

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“…The AFLP-based PCR was carried out as has been previously described (Cires 2011;Cires et al 2011). The genomic DNA was digested with EcoRI and MseI restriction enzymes (New England Biolabs Inc.).…”

Section: Aflp Amplificationmentioning

confidence: 99%

Genetic diversity and structure in fragmented populations of the endangered species Ranunculus cabrerensis (Ranunculaceae): implications for conservation

2012

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Ranunculus cabrerensis is an endemic and endangered species of the Northwestern Iberian Peninsula. The molecular markers AFLP and ISSR were used to investigate the genetic diversity and population structure of four populations across its known distribution. Fifteen selective primer combinations of AFLP and seventeen ISSR primer combinations produced a total of 2830 and 103 unambiguously repeatable fragments respectively, of which 97.57 and 81.38% were polymorphic for both markers. The genetic diversity of R. cabrerensis at species level was high (HE = 0.294 by ISSR and HE = 0.191 by AFLP) and differentiation between sampled locations was also relatively high (GST = 0.316 and 0.158 by ISSR and AFLP analysis respectively) compared to other studies of endangered and rare species using the same techniques. The analysis of molecular variance (AMOVA) indicated that the main genetic variation was within sampled locations (73% by AFLP; 52% by ISSR), even though the variation among locations was also significant. Principal Coordinates, NeighborNet and Bayesian analyses revealed a weak but significant relationship between the genetic structures of different populations in R. cabrerensis, with gene flow acting as a homogenizing force that prevents stronger differentiation of populations. Finally, suggestions for conservation strategies to preserve the genetic resources of this species are outlined.

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Genetic structure in peripheral Western European populations of the endangered species Cochlearia pyrenaica (Brassicaceae)

Cited by 16 publications

References 44 publications

Low genetic and morphological differentiation in the European species complex ofRosa sherardii,R. mollisandR. villosa(RosasectionCaninaesubsectionVestitae)

Low genetic and morphological differentiation in the European species complex ofRosa sherardii,R. mollisandR. villosa(RosasectionCaninaesubsectionVestitae)

Ranunculus×prieti(Ranunculaceae): A new hybrid from the Cantabrian Mountains (North of Spain) revealed by morphological and molecular analyses

Genetic diversity and structure in fragmented populations of the endangered species Ranunculus cabrerensis (Ranunculaceae): implications for conservation

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