Genetic Studies on Herpes Simplex Virus I. Recombination Between Plaque Morphology and Idu (5-Iodo2'-Deoxyuridine) Resistance

Complementation and Recombination Studies on Cell Fusion by Herpes Simplex Virus Type 2 Utilizing Non-fusing Mutants

“…Syncytial mutants of HSV have been reported by a number of investigators (Hoggan and Roizman, 1959;Nii and Kamahora, 1961;Ejercito et al 1968;Yamamoto et al 1972). It has been recognized that the fusion function is genetically determined by the infecting virus and that several genes are responsible for cell fusion (Brown et al 1973;Yamamoto and Kabuta, 1973;Yamamoto and Kabuta, 1976;Manservigi et al 1977;R yechan et al 1979;Honess et al 1980;Little and Schaffer, 1981). On the other hand, certain viral mutants fuse in some cell types and not in others (Ruyechan et al 1979;Palenzone et al 1979;Haf f y and Spear, 1980;Bzik and Person, 1981).…”

Failure of intertypic complementation and recombination between non-fusing mutants of herpes simplex virus. Types 1 and 2.

“…Thus, 1. cells infected with r5 (HSV1) 2. cells infected with G7471 (HSV1) 3. cells infected with G211 (HSV2) 4. cells infected with r5 and G7471 5. cells infected with r5 and G211 6. cells infected with G7471 and G211 Recombination between fresh isolates and G virus of HSV1 and HSV2 * Recombination frequencies (%) were determined by dividing the yield of L type virus by the total yield (Yamamoto and Kabuta, 1976 S was characterized as a small plaque for mation with round cells, L a larger plaque formation with round cells, and G the largest plaque formation with a syncytium produced by extensive cell fusion (Yama moto et al 1972 a). The mixed infection of cells with S and G viruses caused cell rounding and produced L -type recombi nants in the progeny (Yamamoto et al 1972;Yamamoto and Kabuta, 1973;Yamamoto and Kabuta, 1976 Table 6, the yield of the recombinants was significantly lower in mixed infections with heterolo gous type viruses than with homologous ones.…”