Genetic suppression of cryoprotectant toxicity

Cypser, James R.; Chick, Wallace S.; Fahy, Gregory M.; Schumacher, Garrett J.; Johnson, Thomas E.

doi:10.1016/j.cryobiol.2018.11.003

Cited by 8 publications

(4 citation statements)

References 55 publications

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“…In perspective, genetic studies have suggested the possibility of monitoring the susceptibility of the cryopreserved material to cryoprotectant toxicity [ 101 ]. This indicates an opportunity to optimize cryopreservation protocols, driving them in the era of precision medicine.…”

Section: Discussionmentioning

confidence: 99%

Natural Cryoprotective and Cytoprotective Agents in Cryopreservation: A Focus on Melatonin

et al. 2022

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Cryoprotective and cytoprotective agents (Cytoprotective Agents) are fundamental components of the cryopreservation process. This review presents the essentials of the cryopreservation process by examining its drawbacks and the role of cytoprotective agents in protecting cell physiology. Natural cryoprotective and cytoprotective agents, such as antifreeze proteins, sugars and natural deep eutectic systems, have been compared with synthetic ones, addressing their mechanisms of action and efficacy of protection. The final part of this article focuses melatonin, a hormonal substance with antioxidant properties, and its emerging role as a cytoprotective agent for somatic cells and gametes, including ovarian tissue, spermatozoa and spermatogonial stem cells.

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Section: Discussionmentioning

confidence: 99%

Natural Cryoprotective and Cytoprotective Agents in Cryopreservation: A Focus on Melatonin

et al. 2022

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“…For vitrification, a greater understanding of cryoprotectant toxicity is needed, beyond simply ‘more is worse’, which is not always the case. An innovative method for identifying genes involved in cryoprotectant toxicity resistance was developed by creating a library of mutant mESCs and exposing them to 9% M22 (a common vitrification solution) 192 . The investigators identified six mutants that were resistant to M22, with multiple possible pathways involved in cryoprotectant toxicity resistance.…”

Section: Emerging and Future Discovery Methodsmentioning

confidence: 99%

Chemical approaches to cryopreservation

2022

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Cryopreservation of cells and biologics underpins all biomedical research from routine sample storage to emerging cell-based therapies, as well as ensuring cell banks provide authenticated, stable and consistent cell products. This field began with the discovery and wide adoption of glycerol and dimethyl sulfoxide as cryoprotectants over 60 years ago, but these tools do not work for all cells and are not ideal for all workflows. In this Review, we highlight and critically review the approaches to discover, and apply, new chemical tools for cryopreservation. We summarize the key (and complex) damage pathways during cellular cryopreservation and how each can be addressed. Bio-inspired approaches, such as those based on extremophiles, are also discussed. We describe both small-molecule-based and macromolecular-based strategies, including ice binders, ice nucleators, ice nucleation inhibitors and emerging materials whose exact mechanism has yet to be understood. Finally, looking towards the future of the field, the application of bottom-up molecular modelling, library-based discovery approaches and materials science tools, which are set to transform cryopreservation strategies, are also included.

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“…Knowing the suppressed biochemical interactions in the living matter at cryogenic temperatures, the creation of a protective environment for freezing of cells and tissues is feasible by means of gradual (slow) or instant freezing (vitrification) [153]. A serious shortcoming of cryoconservation technology is the deteriorated vitality and structural integrity of the frozen cells/tissues caused by osmotic shocks, exposure to intracellular solutes with high concentrations or toxicity of the chemicals (cryoprotectants) utilized to decrease the freezing point of liquid suspensions and minimize the intracellular ice nucleation events that rupture the cell membrane [154][155][156]. The experiments are performed at Scientific Complex 2 of Bulgarian Academy of Sciences, Sofia, Bulgaria in January 2018.…”

Section: Fundamentally New Potential Applications Of the Passive Icepmentioning

confidence: 99%

From Extremely Water-Repellent Coatings to Passive Icing Protection—Principles, Limitations and Innovative Application Aspects

Esmeryan¹

2020

Coatings

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The severe environmental conditions in winter seasons and/or cold climate regions cause many inconveniences in our routine daily-life, related to blocked road infrastructure, interrupted overhead telecommunication, internet and high-voltage power lines or cancelled flights due to excessive ice and snow accumulation. With the tremendous and nature-inspired development of physical, chemical and engineering sciences in the last few decades, novel strategies for passively combating the atmospheric and condensation icing have been put forward. The primary objective of this review is to reveal comprehensively the major physical mechanisms regulating the ice accretion on solid surfaces and summarize the most important scientific breakthroughs in the field of functional icephobic coatings. Following this framework, the present article introduces the most relevant concepts used to understand the incipiency of ice nuclei at solid surfaces and the pathways of water freezing, considers the criteria that a given material has to meet in order to be labelled as icephobic and clarifies the modus operandi of superhydrophobic (extremely water-repellent) coatings for passive icing protection. Finally, the limitations of existing superhydrophobic/icephobic materials, various possibilities for their unconventional practical applicability in cryobiology and some novel hybrid anti-icing systems are discussed in detail.

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Genetic suppression of cryoprotectant toxicity

Cited by 8 publications

References 55 publications

Natural Cryoprotective and Cytoprotective Agents in Cryopreservation: A Focus on Melatonin

Natural Cryoprotective and Cytoprotective Agents in Cryopreservation: A Focus on Melatonin

Chemical approaches to cryopreservation

From Extremely Water-Repellent Coatings to Passive Icing Protection—Principles, Limitations and Innovative Application Aspects

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