Genetic tracing of the gustatory and trigeminal neural pathways originating from T1R3-expressing taste receptor cells and solitary chemoreceptor cells

Molecular mechanisms underlying the reception and transmission of sour taste information

“…40) Pkd1l3 exhibits specific expression in the TRCs of the CvP and FoP but not in the FuP 18,29) or in the solitary chemoreceptor cells of the nasal epithelium. 35) Pkd1l3-driven WGA has not only confirmed innervation of the CvP and FoP by neurons of CN IX in the NPG, but has also revealed a small number of cells innervated by neurons of CN VII in the GG via the CT, thereby verifying previous electrophysiological reports of these connections in rats. 41) The transgenic mice revealed the sour gustatory pathway from the Pkd1l3-positive soursensing TRCs in the posterior region of the tongue, particularly the peripheral gustatory neurons in the NPG and GG to the central neurons in the NST.…”

“…[4][5][6][7][8][9] The wheat germ agglutinin (WGA) transgene has been used as a transneuronal tracer to label neural pathways that originate from genetically modified cells that express WGA in a variety of nervous systems, including the gustatory system. [35][36][37][38][39] To visualize the gustatory pathway that originates from sour-sensing TRCs in the posterior region of the tongue, transgenic mouse lines expressing WGA in the type-III taste cells of the CvP and FoP under the control of the mouse Pkd1l3 promoter/enhancer have been established. 40) Pkd1l3 exhibits specific expression in the TRCs of the CvP and FoP but not in the FuP 18,29) or in the solitary chemoreceptor cells of the nasal epithelium.…”

Bitter triggers acetylcholine release from polymodal urethral chemosensory cells and bladder reflexes

“…Similarly, solitary nasal chemosensory cells coexpress Tas1R3 with Tas2R5 and Tas2R8 (33); however, although the responses of these cells to bitter stimuli are well documented (34), whether they also respond to umami remains to be established. This situation is in contrast to oropharyngeal gustation, where Tas1R and Tas2R receptors are not coexpressed in taste buds (33,35), and 83% of receptor cells in murine vallate papillae respond to only a single taste quality, which provides a basis for taste coding in taste buds (36). ] i recordings from eGFP + (n = 13, Upper) and eGFP − (n = 74; Lower) isolated urethral cells located in their vicinity (same field of view during confocal laser scanning recording) on the coverslip.…”

Intestinal epithelial tuft cells initiate type 2 mucosal immunity to helminth parasites

“…Single colorimetric and double fluorescent in situ hybridisation analyses were carried 15 out as described previously 30 . Briefly, digoxigenin--and fluorescein--labeled antisense RNAs were synthesized and used as probes after fragmentation to about 150 bases under alkaline conditions.…”