Genetic Transformation

Smith, Hamilton O.; Danner, David B.; Deich, R A

doi:10.1146/annurev.bi.50.070181.000353

Cited by 225 publications

(152 citation statements)

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“…Carlson et al (1983) found 15 kb-sized pieces of DNA to be optimal for transformation of Pseudomonas stutzeri. The average size of integrated transforming DNA for Bacillus subtilis was found to b e 10 kb (Smith et al 1981). Concino (1981, cited in Goodgal 1982 found no transformation with 0.3 k b fragments but low levels at 0.45 k b DNA.…”

Section: Discussionmentioning

confidence: 98%

Distribution and molecular weight of dissolved DNA in subtropical estuarine and oceanic environments

DeFlaun

Paul

Jeffrey³

1987

Mar. Ecol. Prog. Ser.

140

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Dissolved DNA and a series of microbial biomass and activity parameters were measured in offshore, coastal, estuanne and coral reef environments of the southeast Gulf of Mexico. Oceanic concentrations of dissolved DNA ranged from 0.2 to 19 vg 1-' and decreased as a function of distance from shore and depth in the water column. Dissolved DNA concentrations were greater than half the particulate DNA content in offshore environments (Z = 63 + 45 O/O), but were a smaller percentage of particulate DNA in nearshore and estuarine environments (Z = 35 f 21 %). Dissolved DNA correlated better with bacterial parameters (i.e. bacterial direct counts, particulate DNA and thymidine incorporation) than with phytoplankton parameters (chlorophyll a, primary productivity). The molecular weight (MW) of dissolved DNA (determined by agarose gel electrophoresis) ranged from 0.12 kilobase pairs (kb; 7.75 X 104 daltons) to 35.2 kb (2.32 X 10' daltons) for estuarine samples, while an oligotrophic environment contained smaller MW DNA (range 0.24 to 14.27 kb). DNA fragments in this size range are sufficient to contain gene sequences. These results are discussed in terms of the potential for transformation by dissolved DNA.

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Section: Discussionmentioning

confidence: 98%

Distribution and molecular weight of dissolved DNA in subtropical estuarine and oceanic environments

DeFlaun

Paul

Jeffrey³

1987

Mar. Ecol. Prog. Ser.

140

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“…One possibility is that two signals contribute to the species specificity of competence and DNA uptake. In contrast to some naturally competent organisms (e.g., Haemophilus influenzae and Neisseria gonnorhoea), which prefer to take up DNA containing a species-specific sequence (Smith and Danner 1981;Stewart 1989), B. subtilis will bind and take up DNA of any sequence. Two signals could help to ensure that competence is induced only in the presence of other B.…”

Section: Discussionmentioning

confidence: 99%

Convergent sensing pathways mediate response to two extracellular competence factors in Bacillus subtilis.

Solomon¹,

Magnuson²,

Srivastava³

et al. 1995

Genes Dev.

185

163

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Development of genetic competence in Bacillus subtilis is regulated by extracellular signaling molecules, including the ComX pheromone, a modified 9-or 10-amino-acid peptide. Here, we present characterization of a second extracellular competence stimulating factor (CSF). CSF appears to be, at least in part, a small peptide of between 520 and 720 daltons. Production of CSF requires several genes that are needed both for initiation of sporulation and development of competence (spoOH, spoOA, spoOB, and spoOF).

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“…The transformation process provides a reliable method for introduction of foreign DNA into strains of Lactobacillus. The process of bacterial transformation has been reviewed extensively (Mercenier and Chassy, 1988;Saunders et al, 1984;Smith et al, 1981;Stewart and Carlson, 1986;Venem~, 1979). A cell can be transformed by several techniques to make it competent.…”

Section: Transformationmentioning

confidence: 99%